Labworks software version 4

Total protein was prepared as described before (Hagenbuchner et al., 2016a (link)). Proteins were separated by SDS-PAGE and blotted on nitrocellulose membrane (GE Healthcare, Chalfot, UK). After blocking, membranes were incubated with primary antibodies directed against Bim, Noxa, DEPP1, SESN3, FOXO3, GAPDH or αTubulin (Supplementary file 2), washed and incubated with horseradish-peroxidase conjugated secondary antibody (GE Healthcare, UK). The immunoblots were developed by enhanced chemiluminescence (Merck, Vienna, Austria) according to manufacturer’s instructions and analyzed using an AutoChemi detection system (UVP, Cambridge, UK). Densitometry was performed using Labworks software version 4.5 (UVP, UK).

Total protein was prepared as described in Hagenbuchner et al.^{21 (link)}. Proteins were separated by SDS-PAGE and blotted on nitrocellulose membrane (GE Healthcare, Chalfot, UK). After blocking, membranes were incubated with primary antibodies against MFN1, MFN2, DNM1L, and OXPHOS (Abcam, Cambridge, UK), pDNM1L(Ser637) and α-Tubulin (Cell Signaling Technology Inc., Boston, USA), and GAPDH (Acris, Herford, Germany) washed and incubated with horseradish-peroxidase conjugated secondary antibody (GE Healthcare, UK). The immunoblots were developed by enhanced chemiluminescence (GE Healthcare, Chalfont, UK) according to manufacturer’s instructions and analyzed using an AutoChemi detection system (UVP, Cambridge, UK). Densitometry was performed using Labworks software version 4.5 (UVP, UK). Uncropped blots of three independently generated panels are shown in Supplemental Fig. 1.