Mouse ccl2 je mcp 1 duoset

Oxidative stress and inflammation are associated with metal dyshomeostasis and neurodegeneration (Barnham et al., 2004 (link); Molina-Holgado et al., 2007 (link)). To assess the oxidative and inflammatory status of the brain and spinal cord of the TDP-43^A315T we measured levels of oxidized proteins and the inflammation marker monocyte chemoattractant protein 1 (MCP-1). Brain and spinal cord soluble and insoluble fractions were analyzed for oxidative modified proteins via the OxyBlot Protein Oxidation Detection Kit (Merck Millipore, Australia) according to the manufacturer’s instructions. The OxyBlot detects the carbonyl groups found on oxidized proteins. The OD of bands was quantified using the ImageJ software and values were standardized to the loading controls GAPDH and histone H3 for soluble and insoluble fractions, respectively. The relative fold change of oxidized proteins in TDP-43^A315T mice is expressed relative to wild-type.Levels of MCP-1 were measured in brain, spinal cord, liver and quadriceps muscle using the Mouse CCL2/JE/MCP-1 DuoSet (R&D Systems, USA) according to the manufacturer’s instructions.