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Anti phospho rsk

Manufactured by Santa Cruz Biotechnology

Anti-phospho-RSK is a lab equipment product that detects and quantifies the phosphorylated form of RSK (Ribosomal S6 Kinase), a protein kinase involved in cellular signaling pathways. This product enables researchers to analyze the activation status of RSK in their experimental systems.

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2 protocols using anti phospho rsk

1

Comprehensive Antibody Analysis Protocol

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The following antibodies were used for IP and/or western blot analysis: anti-β-actin (#4970; Cell Signalling), anti-CDK9 (sc-13130; Santa Cruz), anti-CNOT1 (14276-1-AP; Protein Tech), anti-CNOT7 (H00029883; Abnova), anti-FLAG (M2; Sigma), anti-hnRNPD (Q14103; Millipore), anti-HA (1867423; Roche), anti-IGF2BP1 (sc-21026; Santa Cruz), anti-KHSRP (ab83291; Abcam), anti-LARP7 (LaRP7-101AP; FabGennix Inc.), anti-LDLR (AF2148; BD Biosciences), anti-Myc (9E10; Roche), anti-phospho-ERK (#9101s; Cell Signalling), anti-phospho-MAPKAPK2 (#3007s; Cell Signalling), anti-phospho-RSK (sc-17033; Santa Cruz), anti-ZFP36L1 (#2119; Cell Signalling), anti-phospho-S6 (#2211; Cell Signalling).
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2

Signaling Pathways Regulating Cell Proliferation

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Human PTHrP (1-34) and high glucose Dulbecco's modified Eagle's medium (DMEM) were obtained from Sigma-Aldrich Chemical. Fetal bovine serum (FBS) was from Natocord (Córdoba, Argentina). Antibodies were from the following sources: anti-RSK1/2/3, anti-FAK, anti-cyclin D1, anti-CDK-6 and anti-p53 were from Cell Signaling Technology. Anti-phospho RSK, anti-GAPDH, anti-phospho tyrosine antibody, goat anti-rabbit peroxidase-conjugated secondary antibody and goat antimouse peroxidase-conjugated secondary antibody were from Santa Cruz Biotechnology. PD 98059 and SB 203580 were from Calbiochem. SL0101 was from Santa Cruz Biotechnology. SB 202190 was from Tocris Bioscience (United Kingdom). Crystal violet was from MERCK. Protein size markers were from Amersham Biosciences, PVDF (Immobilon polyvinylidene difluoride) membranes and ECL chemiluminescence detection kit were from Amersham. Transwell filters were with the following features: polyethylene terephthalate (PET), 8 μm pores,
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