M724001 2

Manufactured by Agilent Technologies

Sourced in Belgium

The M724001-2 is a laboratory equipment product from Agilent Technologies. It is designed for analytical measurement and testing purposes. The product's core function is to provide accurate and reliable data for research, development, or quality control applications. No further details or interpretation about the intended use of this product are provided.

Automatically generated - may contain errors

Lab products found in correlation

Apoptag s7110, by Merck Group (2 mentions) Axioskop 2 microscope, by Zeiss (1 mentions) Ds l1 camera, by Nikon (1 mentions) Sm2000r, by Leica (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) Smi 32, by BioLegend (1 mentions) Mab377, by BioLegend (1 mentions) Mab360, by Swant (1 mentions) Poly l lysine coated slide, by Merck Group (1 mentions) Cresyl violet, by Merck Group (1 mentions) Mab377, by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Alexa fluor 488 goat anti mouse conjugate, by Thermo Fisher Scientific (1 mentions) Mab5384, by Merck Group (1 mentions)

4 protocols using m724001 2

Immunohistochemical Analysis of Tumor Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumors from control and treated groups previously grown in the animal model were fixed in 10% (v/v) buffered formalin for at least 48 h and routinely processed for paraffin embedding. Serial 4 μm-thick sections were cut for hematoxylin and eosin (H&E) stain and immunohistochemistry for the proliferation marker Ki-67. Briefly, the sections were incubated with anti-Ki-67 (M724001-2, 1:100, DAKO, Glostrup, Denmark) for 1 h at room temperature. Antigen retrieval was performed in citrate buffer, pH 6, according to the antibody manufacturer. Sections were then incubated with a Double stain System, Rabbit/Mouse (DAB+/Permanent Red) (DAKO, Glostrup, Denmark). Slides were examined by light microscopy in an Axioskop 2 Zeiss microscope (Carl Zeiss, Jena, Germany) and photographs acquired using a Nikon DS-L1 camera (Nikon, Tokyo, Japan) with 200× magnification. A minimum of 1000 cells were evaluated per slide.

Lima R.T., Sousa D., Gomes A.S., Mendes N., Matthiesen R., Pedro M., Marques F., Pinto M.M., Sousa E, & Vasconcelos M.H. (2018). The Antitumor Activity of a Lead Thioxanthone is Associated with Alterations in Cholesterol Localization. Molecules, 23(12), 3301.

+ Open protocol

+ Expand

Immunohistochemical Profiling of Neural Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue slices (350 µm-thick) designated for histological profiling were fixed for 2–4 days in 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS) at 4 °C and transferred to PBS, 0.1% sodium azide for storage at 4 °C. Slices were then cryoprotected in 30% sucrose, frozen and re-sectioned at 30 µm using a sliding microtome (Leica SM2000R). Sections were stored in PBS with azide at 4 °C in preparation for immunohistochemical and Nissl staining. Specific probes (vendor, dilution) used were: Neu-N (Millipore, MAB377, 1:2,000); SMI-32 (Biolegend, 801704, 1:2,000); GFAP (Millipore, MAB360, 1:1,500); parvalbumin (Swant, PV235, 1:2,000); IBA1 (Wako 019-19741, 1:1,000); Ki-67 (Dako M724001-2, 1:200). Full immunohistology protocol details available at http://help.brain-map.org/download/attachments/8323525/CellTypes_Morph_Overview.pdf?version=4&modificationDate=1528310097913&api=v2

Berg J., Sorensen S.A., Ting J.T., Miller J.A., Chartrand T., Buchin A., Bakken T.E., Budzillo A., Dee N., Ding S.L., Gouwens N.W., Hodge R.D., Kalmbach B., Lee C., Lee B.R., Alfiler L., Baker K., Barkan E., Beller A., Berry K., Bertagnolli D., Bickley K., Bomben J., Braun T., Brouner K., Casper T., Chong P., Crichton K., Dalley R., de Frates R., Desta T., Lee S.D., D’Orazi F., Dotson N., Egdorf T., Enstrom R., Farrell C., Feng D., Fong O., Furdan S., Galakhova A.A., Gamlin C., Gary A., Glandon A., Goldy J., Gorham M., Goriounova N.A., Gratiy S., Graybuck L., Gu H., Hadley K., Hansen N., Heistek T.S., Henry A.M., Heyer D.B., Hill D., Hill C., Hupp M., Jarsky T., Kebede S., Keene L., Kim L., Kim M.H., Kroll M., Latimer C., Levi B.P., Link K.E., Mallory M., Mann R., Marshall D., Maxwell M., McGraw M., McMillen D., Melief E., Mertens E.J., Mezei L., Mihut N., Mok S., Molnar G., Mukora A., Ng L., Ngo K., Nicovich P.R., Nyhus J., Olah G., Oldre A., Omstead V., Ozsvar A., Park D., Peng H., Pham T., Pom C.A., Potekhina L., Rajanbabu R., Ransford S., Reid D., Rimorin C., Ruiz A., Sandman D., Sulc J., Sunkin S.M., Szafer A., Szemenyei V., Thomsen E.R., Tieu M., Torkelson A., Trinh J., Tung H., Wakeman W., Waleboer F., Ward K., Wilbers R., Williams G., Yao Z., Yoon J.G., Anastassiou C., Arkhipov A., Barzo P., Bernard A., Cobbs C., de Witt Hamer P.C., Ellenbogen R.G., Esposito L., Ferreira M., Gwinn R.P., Hawrylycz M.J., Hof P.R., Idema S., Jones A.R., Keene C.D., Ko A.L., Murphy G.J., Ng L., Ojemann J.G., Patel A.P., Phillips J.W., Silbergeld D.L., Smith K., Tasic B., Yuste R., Segev I., de Kock C.P., Mansvelder H.D., Tamas G., Zeng H., Koch C, & Lein E.S. (2021). Human neocortical expansion involves glutamatergic neuron diversification. Nature, 598(7879), 151-158.

+ Open protocol

+ Expand

Histological Analysis of Brain Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brains were microtomed at 4 µm thickness from anterior to posterior. A set of 5 serial coronal sections at 100 µm intervals was taken at each of the following two levels. The first level started at the medial septal nucleus and second at the hippocampal formation. Supplementary Fig. 3 illustrates the regions of interest. Sections were placed onto poly-L- lysine coated slides (Sigma-Aldrich, Bornem, Belgium). Staining was done with Cresyl Violet (C5042-10G, Sigma-Aldrich, Overijse, Belgium) and the primary antibodies used included mouse monoclonal anti-NeuN antibody (MAB377, Millipore, Billerica, MA, USA), anti-NG2 chondroitin sulfate proteoglycan antibody (MAB5384, Millipore, Billerica, MA, USA), mouse monoclonal anti-glial fibrillary acidic protein antibody (GFAP) (G6171, Sigma-Aldrich, St Louis, MO, USA), mouse monoclonal anti-human Ki67 (M724001-2, Agilent, Diegem, Belgium), mouse monoclonal anti-synaptophysin (Sy38, ab8049, Abcam, Cambridge, UK). The secondary antibody was Alexa Fluor® 488 goat anti-mouse conjugate (Invitrogen). Degenerating nuclei were visualized by a terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) method for fluorescent in situ end labelling of double-stranded DNA fragmentation (Apoptag S7110; Millipore, Billerica, MA, USA). Sections were counterstained with Hoechst 33342 (Sigma-Aldrich, Bornem, Belgium).

van der Merwe J., van der Veeken L., Ferraris S., Gsell W., Himmelreich U., Toelen J., Ourselin S., Melbourne A., Vercauteren T, & Deprest J. (2019). Early neuropathological and neurobehavioral consequences of preterm birth in a rabbit model. Scientific Reports, 9, 3506.

+ Open protocol

+ Expand

Perfusion Fixation and Brain Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

On day 1 or day 56, animals were transcardially perfusion fixated after ketamine/xylazin sedation (ketamine (35 mg/kg IM) and xylazin (6 mg/kg IM), XYL-M®; VMD, Arendonk, Belgium). Brains were extracted, paraffin embedded and serially sectioned at four µm every 50µm as described before 19 . Sections were stained with Cresyl Violet (C5042-10G, Sigma-Aldrich, Overijse, Belgium), mouse monoclonal anti-human Ki67 (M724001-2, Agilent, Diegem, Belgium), mouse monoclonal anti-synaptophysin (Sy38, ab8049, Abcam, Cambridge, UK) and a terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) method for fluorescent in situ end labelling of double-stranded DNA fragmentation (Apoptag S7110; Millipore, Billerica, MA, USA). Four relevant brain areas were evaluated, i.e. the prefrontal cortex, caudate nucleus, hippocampus and thalamus. Further details and quantification methods can be found in Supplement4.

Van der Veeken L., Van der Merwe J., Devroe S., Inversetti A., Galgano A., Bleeser T., Meeusen R., Rex S, & Deprest J. (2019). Maternal surgery during pregnancy has a transient adverse effect on the developing fetal rabbit brain. American journal of obstetrics and gynecology, 221(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!