Clarity and clarity max western ecl substrates

Manufactured by Bio-Rad

Clarity and Clarity Max Western ECL Substrates are chemiluminescent detection reagents designed for the sensitive and quantitative detection of proteins on Western blots. These substrates produce a luminescent signal upon reaction with horseradish peroxidase (HRP), which is commonly used as a reporter enzyme in Western blot analysis.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Mouse anti β actin, by Cell Signaling Technology (1 mentions) Hrp conjugated donkey anti rabbit igg, by GE Healthcare (1 mentions) Hrp conjugated sheep anti mouse igg, by GE Healthcare (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Catalase, by ABclonal (1 mentions) P stat1, by Cell Signaling Technology (1 mentions) P jak2, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by ABclonal (1 mentions) Beta actin β actin, by Santa Cruz Biotechnology (1 mentions) Icam 1, by Proteintech (1 mentions) P c jun, by Cell Signaling Technology (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) Agarose, by Bio-Rad (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Senescence β galactosidase staining kit, by Cell Signaling Technology (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Clarity Western ECL Substrate (2707 citations) ECL substrate (255 citations) Clarity Western ECL (200 citations) Clarity ECL (155 citations) Clarity ECL substrate (143 citations) Western ECL Substrate (80 citations) Clarity Max (38 citations) Clarity Max ECL (17 citations) Clarity Max substrate (5 citations) Clarity and Clarity Max ECL (4 citations)

3 protocols using clarity and clarity max western ecl substrates

Western Blot Analysis of Desmoglein-2

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Samples were separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore) using overnight wet transfer (48mM Tris pH 9.2, 38mM glycine, 20% methanol, 0.05% SDS). After blocking for 6–8 h with 5% milk in PBS-T, blots were probed with rabbit anti-desmoglein-2 (1:250 dilution, A303-758A; Bethyl Labs) and mouse anti-β-actin (1:100,000 dilution, 3700; Cell Signaling) in PBS-T with 5% milk overnight. Blots were then secondarily probed with HRP-conjugated sheep anti-mouse IgG (1:1000 dilution, NA931V; GE) and HRP-conjugated donkey anti-rabbit IgG (1:1000 dilution, NA934V; GE) for 1 h. Gels were developed using Clarity and Clarity Max Western ECL substrates (1705060 and 1705062; BioRad). Western blot images were analyzed with ImageJ.

McLellan L.K., McAllaster M.R., Kim A.S., Tóthová Ľ., Olson P.D., Pinkner J.S., Daugherty A.L., Hreha T.N., Janetka J.W., Fremont D.H., Hultgren S.J., Virgin H.W, & Hunstad D.A. (2021). A host receptor enables type 1 pilus-mediated pathogenesis of Escherichia coli pyelonephritis. PLoS Pathogens, 17(1), e1009314.

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Western Blot Quantification of Cellular Proteins

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According to the procedures performed in a previous study [9 (link)], western blotting was conducted using primary antibodies against the following proteins: BAX, BCL-2, catalase (CAT), caspase-12 (ABclonal, Boston, MA, USA); cleaved caspase-3, caspase-3, CHOP, cleaved caspase-8, caspase-8, p-NF-κB, NF-κB, p-SAPK/JNK (p-JNK, Thr183/Tyr185), JNK, p-c-Jun (p-c-Jun, Ser73), c-Jun, p-Akt (p-Akt, Thr308), Akt, p-Bad (p-Bad, Ser136), Bad, proliferating cell nuclear antigen (PCNA), p-STAT1 (p-STAT1, Tyr701), STAT1, p-JAK2 (p-JAK2, Tyr1007/1008), JAK2 (Cell Signaling Technology, Beverly, MA, USA); tumor necrosis factor-alpha (TNF-α), IRE1 (Abcam, Cambridge, MA, USA); NAD(P)H dehydrogenase [quinone] 1 (NQO-1), superoxide dismutase 2 (SOD2), beta-actin (β-actin) (Santa Cruz, Dallas, TX, USA); intercellular adhesion molecule 1 (ICAM-1, Proteintech, Chicago, IL, USA). Membranes were then incubated with secondary antibodies (goat anti-mouse IgG or goat anti-rabbit IgG) at room temperature for 1 h and then visualized using an ECL kit (Clarity and Clarity Max Western ECL Substrates, cat#1705060, Bio-Rad). Band densities were quantified using β-actin as the internal control for normalization.

Xiong L., Zhou B., Young J.L., Xu J., Wintergerst K, & Cai L. (2022). Effects of Whole-Life Exposure to Low-Dose Cadmium with Post-Weaning High-Fat Diet on Offspring Testes in a Male Mouse Model. Chemico-biological interactions, 353, 109797.

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MTT Assay for Cell Viability

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The 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). Propidium iodide (PI) were supplied from Sigma-Aldrich Corporation (St. Louis, MO, USA). The RPMI 1640, DMEM, phosphate-buffered saline (PBS) pH 7.4, trypsin, L-glutamine, fetal bovine serum (FBS) and penicillin/streptomycin solution were bought from Gibco (Gaithersburg, MA). Agarose was supplied from Bio-Rad (Hercules, CA, USA). Clarity and Clarity Max Western ECL Substrates (cat: 1705060) is obtained from Bio-Rad. Pierce BCA Protein Assay Kit were obtained from Thermo Fisher Scientific (Rockford, IL, USA). CDDP (Pt(NH3)2Cl2) was purchased from Sigma-Aldrich Corporation. Velcade (Bortezomib) was kindly provided by Dr. Sudjit Luanpitpong, Mahidol University, Bangkok, Thailand. The G418 sulfate, Neomycin (50 mg/mL) was obtained from Thermo Fisher Scientific. The IT139, the GRP78 inhibitor was supplied from Adooq Bioscience, USA. The senescence β-galactosidase staining kit (cat no: #9860) and primary antibodies kit for ER stress (cat no: #9956) was purchased from cell signaling Technology (USA). The cellular senescence marker and secretory phenotype PCR primers were obtained from Macrogen, Korea.

Ei Z.Z., Choochuay K., Tubsuwan A., Pinkaew D., Suksomtip M., Vinayanuwattikun C., Chanvorachote P, & Chunhacha P. (2021). GRP78/BiP determines senescence evasion cell fate after cisplatin-based chemotherapy. Scientific Reports, 11, 22448.

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