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3 protocols using carbon dioxide co2

1

Chemical Reagents for Organic Synthesis

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Allyl-alcohol (99%), benzyl alcohol (99%), butanol (99.5%), 1-octanol (99%), phenyl acetaldehyde (98%, stabilized) and trimethylamine (99.7%) were purchased from Acros Organics. 1-Adamantyl isocyanide (95%), cyclohexanol (99%), cyclohexyl isocyanide (98%), 2-butanol (99.5%, anhydrous), 2,4-dinitrobenzaldehyde (97%), isobutyraldehyde (≥99.5%), 2-morpholinoethyl isocyanide (≥98%), tert-butanol (≥99.5%, anhydrous), tert-butyl isocyanide (98%), tetradecane (≥99.5%) and undecylenic aldehyde (95%) were obtained from Sigma Aldrich. Other chemicals used include: diazabicyclo[5.4.0]undec-7-ene (DBU, TCl, >98%), deuterated chloroform (CDCI3-d, Merck), dimethyl sulfoxide (DMSO, VWR, 99%) and trimethyl acetaldehyde (ABCR, 97%). Carbon dioxide (CO2) with purity over 99.9% was obtained from Air Liquide. Cyclohexane, ethyl acetate and dichloromethane were distilled before usage, while tetrahydrofuran (THF) was of technical grade and used without further purification. All other chemicals were used as received from the supplier.
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2

Purification of Nitrogenase MoFe Proteins

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All reagents were obtained from SigmaAldrich (St.Louis, MO) or Fisher Scientific (Fair Lawn, NJ) and were used without further purification. Carbon dioxide (CO2) was purchased from Air Liquide (Walnut Creek, CA) and methane from Air Gas (Radnor, PA). Azotobacter vinelandii strains DJ995 (wild-type), DJ997 (α-195Gln), DJ1310 (α-70Ala), and DJ1316 (α-70Ala/α-195Gln) were grown, and the corresponding nitrogenase MoFe proteins having a seven-His tag addition near the carboxyl-terminal end of the α-subunit, were expressed and purified as previously described.22 (link) Protein concentrations were determined by the Biuret assay using bovine serum albumin as standard. Handling of proteins and buffers was done in septum-sealed serum vials under an argon atmosphere or on a Schlenk vacuum line. All gases and liquids were transferred using gas-tight syringes.
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3

Functionalized Graphene Nanosheets for CO2 Capture

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PET pellets (CB602) were purchased from Far Eastern Industries (Shanghai). GNs (layer number of 5 to 6, flake diameter of 100 nm to 3 μm, and specific surface area greater than 400 m2 g−1) was provided by Shanxi Institute of Coal Chemistry, Chinese Academy of Sciences. γ-Aminopropyl triethoxysilane (KH550), γ-glycidyloxy propyl trimethoxysilane (KH560), and γ-methacryloxy propyl trimethoxysilane (KH570) were obtained from Zhongjie Chemical Technology Company (Guangzhou). Ethanol and acetic acid were purchased from Sinopharm Chemical Reagent Company. Carbon dioxide (CO2) was obtained from Air Liquide, (Guiyang, China) (purity 99.9%).
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