Mouse and rabbit horseradish peroxidase antibodies

Manufactured by Cytiva

Sourced in United States

Mouse and rabbit horseradish peroxidase antibodies are immunological reagents used in various laboratory techniques. They consist of antibodies derived from mice or rabbits that are conjugated with the enzyme horseradish peroxidase. These antibodies can be utilized to detect and quantify target proteins in samples.

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2 protocols using mouse and rabbit horseradish peroxidase antibodies

Antibody Sources for Western Blot

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Primary antibodies were obtained from the following sources: Na⁺/K⁺ ATPase‐α, calnexin, calpain 2, CDK2, cyclin B1, cyclin E, ERK2, lamin B, HA tag, p21, p27^Kip1, p57^Kip2, β‐tubulin from Santa Cruz Biotechnology (Santa Cruz, CA), β‐actin from Chemicon International (Billerica, MA), phospho‐AKT (Ser473), AKT, phosphorylated ERK1/2 (T202/Y204), and Lamp1 from Cell Signaling Technology (Danvers, MA) and Cathepsin B from R&D Systems (Mississauga, ON, Canada). Mouse and rabbit horseradish peroxidase antibodies were purchased from Amersham Biosciences (Pittsburg, PA), goat horseradish peroxidase antibody from Santa Cruz, and alkaline phosphatase‐conjugated antibodies were purchased from Promega (Madison, WI).

Bian B., Mongrain S., Cagnol S., Langlois M., Boulanger J., Bernatchez G., Carrier J.C., Boudreau F, & Rivard N. (2015). Cathepsin B promotes colorectal tumorigenesis, cell invasion, and metastasis. Molecular Carcinogenesis, 55(5), 671-687.

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Comprehensive Antibody Characterization Protocol

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Antibodies. Primary antibodies were obtained from the following sources: SHP-2, MEK1/2, ERK2, phospho-RAF1 (Y340/Y341), RAF1, KI67 and γH2AX from Santa Cruz Biotechnology (Santa Cruz, CA, USA), phospho-ERK1/2 (T202/Y204) from Sigma-Aldrich (Mississauga, ON, Canada), β-actin from Chemicon International (Billerica, MA, USA), GAPDH (HRP conjugate), phospho-RELA (S536), RELA, phospho-STAT3 (Y705), STAT3, phospho-RAF-1 (S259), phospho-RAF-1 (S338), non-phospho (active) β-catenin, phospho-AKT (S473), AKT, acetylated-p53 (K379) and phospho-MEK1/2 (S218/S222) from Cell Signaling Technology (Danvers, MA, USA), total p53 and mutant-p53 (R273H) from Abcam (Toronto, ON, Canada) and β-catenin from BD Pharmingen (Mississauga, ON, Canada). Mouse and rabbit horseradish peroxidase antibodies were purchased from Amersham Biosciences (Pittsburg, PA, USA). All other materials were from Sigma-Aldrich (Oakville, ON, Canada) unless stated otherwise.

Gagné-Sansfaçon J., Coulombe G., Langlois M.J., Langlois A., Paquet M., Carrier J., Feng G.S., Qu C.K, & Rivard N. (2016). SHP-2 phosphatase contributes to KRAS-driven intestinal oncogenesis but prevents colitis-associated cancer development. Oncotarget, 7(40), 65676-65695.

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