The antibodies and antibiotic product codes used are as follows: anti-CLPB (Aviva, catalog# ARP53790_P050) 0.5 µg/mL, anti-DnaK (Aviva, catalog# OAED00201) 1 µg/mL, anti-TF (Clontech, catalog# M201) 2 µg/mL, anti-groEL (Abcam, catalog# ab82592) 1 µg/mL, and anti-DnaJ (Enzo Life Sciences, catalog# ADI-SPA-410-D) 0.5 µg/mL. Ampicillin sodium, CAS number 69-52-3 (Duchefa Biochemie, catalog# A0104), erythromycin, CAS number 114-07-8 (Sigma-Aldrich, catalog# E5389), chloramphenicol, CAS number 56-75-7 (Duchefa Biochemie), and kanamycin CAS number 56-75-7 (Duchefa Biochemie).
Anti groel
Manufactured by Abcam
Sourced in United States
Anti-groEL is a laboratory reagent used in various research and diagnostic applications. It functions as an antibody that specifically binds to the groEL protein, which is a molecular chaperone involved in protein folding. The core function of Anti-groEL is to detect and measure the presence of groEL in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Erythromycin, by Merck Group (1 mentions)
Kanamycin, by Duchefa Biochemie (1 mentions)
Chloramphenicol, by Duchefa Biochemie (1 mentions)
5z 7 oxozeaenol, by Merck Group (1 mentions)
Hrp conjugated goat anti rabbit igg, by Southern Biotech (1 mentions)
Prl tk, by Takara Bio (1 mentions)
Hrp conjugated goat anti mouse igg, by Southern Biotech (1 mentions)
Pnf kb luc, by Takara Bio (1 mentions)
2 protocols using anti groel
1
Molecular Chaperone Immunoblotting Assay
2
Investigating NF-κB Signaling Pathway Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The plasmids pRv1804c-HA, pIκBα-Flag (FL), pIκBα-F1-Flag (F1), pIκBα-F2-Flag (F2), and pIκBα-F3-Flag (F3) were constructed at our laboratory using specific primers. The plasmid encoding p-ubiquitin-Myc was provided by Prof. Hui Zheng (Soochow University, China). Furthermore, pNF-kB-luc and pRL-TK, dual-luciferase reporter assay vectors, were purchased from Clontech (Mountain View, CA, USA).
The primary antibodies used were anti-NF-κB p65 (CST, 8242), anti-phosphorylated-NF-κB p65 (p- NF-κB p65, CST, 3033), anti-IκBα (9242, CST), anti-p-IκBα (2859, CST), anti-HA (3724, CST), anti-His (CST, 12698), anti-IKKα (CST, 2682), anti-phosphorylated IKKα (p-IKKα, CST, 2697), anti-GroEL (Abcam, ab82592), and anti-β-actin (CST, 3700). The secondary antibody used was horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Southern-Biotech, 1091–05) and HRP-conjugated goat anti-rabbit IgG (Southern-Biotech, 4030–05). In addition, the inhibitors used were JSH-23 (MCE, HY-13982), adezmapimod (MCE, HY-10256), SP600125 (MCE, HY-12041), PD98059 (MCE, HY-12028), (5Z)-7-oxozeaenol (OZ; Sigma, O9890), and BAY-11–7085 (MCE, HY-10257). Monoclonal mouse anti-HA agarose (SAE0197) was purchased from Sigma.
The primary antibodies used were anti-NF-κB p65 (CST, 8242), anti-phosphorylated-NF-κB p65 (p- NF-κB p65, CST, 3033), anti-IκBα (9242, CST), anti-p-IκBα (2859, CST), anti-HA (3724, CST), anti-His (CST, 12698), anti-IKKα (CST, 2682), anti-phosphorylated IKKα (p-IKKα, CST, 2697), anti-GroEL (Abcam, ab82592), and anti-β-actin (CST, 3700). The secondary antibody used was horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Southern-Biotech, 1091–05) and HRP-conjugated goat anti-rabbit IgG (Southern-Biotech, 4030–05). In addition, the inhibitors used were JSH-23 (MCE, HY-13982), adezmapimod (MCE, HY-10256), SP600125 (MCE, HY-12041), PD98059 (MCE, HY-12028), (5Z)-7-oxozeaenol (OZ; Sigma, O9890), and BAY-11–7085 (MCE, HY-10257). Monoclonal mouse anti-HA agarose (SAE0197) was purchased from Sigma.
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