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Recombinant human fgf7

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Recombinant human FGF7 is a growth factor protein produced in a laboratory setting. It is a member of the fibroblast growth factor family and is involved in the regulation of cell growth and differentiation.

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Lab products found in correlation

Dasatinib, by Merck Group (1 mentions) Sb202190, by Merck Group (1 mentions) Ag1478, by Abcam (1 mentions) 1 thioglycerol, by Merck Group (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Corning (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Chir99021, by Apexbio (1 mentions)

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Recombinant FGF7 (4 citations)

2 protocols using recombinant human fgf7

1

Metalloprotease Inhibitor DPC 333 Protocol

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The metalloprotease inhibitor DPC 333 ((2R)‐2‐((3R)‐3‐amino‐3 (4‐[2‐methyl‐4‐quinolinyl) methoxy] phenyl)‐2‐oxopyrrolidinyl)‐N‐hydroxy‐4‐methylpentanamide)) (DPC) was a gift from Dr. Carl P. Blobel (Weill Cornell Medicine, Graduate School of Medical Sciences, NY, USA) and diluted in DMSO to the indicated concentrations. The following intracellular signalling inhibitors were used: AG1478 (#141438, Abcam, USA); DAPT (#D5942), SB202190 (#S7067), CRM197 (#D2189), Dasatinib (#CDS023389) and G1254023X (#SML0789) obtained from MilliporeSigma, USA; LY294002 (#154447‐36‐6) and U0126 (#109511‐58‐2) obtained from Calbiochem, USA and recombinant human FGF7 (# 251‐KG, R&D Systems, USA).
Dixit G., Gonzalez‐Bosquet J., Skurski J., Devor E.J., Dickerson E.B., Nothnick W.B., Issuree P.D., Leslie K.K, & Maretzky T. (2023). FGFR2 mutations promote endometrial cancer progression through dual engagement of EGFR and Notch signalling pathways. Clinical and Translational Medicine, 13(5), e1223.
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2

Bud Tip Organoid Culture Conditions

Cited 1 time
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Bud tips were established for a minimum of 1 passage to ensure epithelial-only cultures before experiments began. Bud tip organoids were passaged 3 days prior to the start of the experiment. On the day of the experiment, media conditions were changed from the original, published bud tip media (Miller et al. 2018 (link)) consisting of DMEM/F-12 (Corning, Cat#10-092-CV), 100U/mL penicillin-streptomycin (Thermo Fisher, Cat#15140122), 1 bottle B-27 supplement (Thermo Fisher, Cat#17504044), 1 bottle N-2 supplement (Thermo Fisher, Cat#17502048), 0.05% BSA (Sigma, Cat#A9647) and final concentrations of 50µg/mL L-ascorbic acid (Sigma, Cat#A4544), 0.4µM 1-Thioglycerol (Sigma, Cat#M1753), 50nM all trans retinoic acid (Sigma, Cat#R2625), 10ng/mL recombinant human FGF7 (R&D Systems, Cat#251-KG), and 3µM CHIR99021 (APExBIO, Cat#A3011). Positive control bud tips were given the described media and negative control bud tips had CHIR99021 removed. Experimental conditions were given either 500ng/mL recombinant human R-Spondin 2 Protein (R&D Systems, Cat#3266-RS) and/or 1X Afamin/WNT3a conditioned media (MBL International Corporation, Cat#J2-001) in place of CHIR99021. Cultures were fed again on day 3 and collected on day 4 for RT-qPCR analysis. Three unique biological specimens with 1 technical replicate per condition each were used for RT-qPCR analysis.
Hein R.F., Wu J.H., Holloway E.M., Frum T., Conchola A.S., Tsai Y.H., Wu A., Fine A.S., Miller A.J., Szenker-Ravi E., Yan K.S., Kuo C.J., Glass I., Reversade B, & Spence J.R. (2022). R-SPONDIN2+ Mesenchymal Cells Form the Bud Tip Progenitor Niche During Human Lung Development. Developmental cell, 57(13), 1598-1614.e8.
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