Total RNA was isolated with TRIzol from ear samples, and reverse transcription and pre-amplification were performed with Reverse Transcription Master Mix and Preamp Master Mix (Fluidigm, South San Francisco, CA, USA), respectively. Quantitative real-time PCR was carried out in the Dynamic Arrays integrated fluidic circuit of a BioMarkHD system (Fluidigm) with specific primer pairs as indicated in Supplementary Table T1
Dynamic arrays
Manufactured by Standard BioTools
Dynamic Arrays are a type of lab equipment designed for high-throughput, parallel processing of samples. They enable the simultaneous analysis of multiple parameters in a single experiment, facilitating efficient and cost-effective data generation.
Automatically generated - may contain errors
2 protocols using dynamic arrays
1
Targeted Gene Expression Analysis
2
SNP Genotyping with 96.96 Dynamic Arrays
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The SNP genotyping procedure using 96.96 Dynamic Arrays™ with integrated fluidic circuits (IFCs)91 (link) was conducted according to the manufacturer’s protocol for genotyping with SNPtype™ Assays (Advanced Development Protocol 34, Fluidigm corp.). Low DNA samples were pre-amplified in a modified STA for enrichment of the target loci before the SNP genotyping PCR. The pre-amplification of the target regions was conducted using 14 cycles for invasive samples and 28 cycles with extracts from non-invasive samples according to von Thaden et al.25 (link).
All experiments and sample setups included NTCs (no template controls) and STA NTCs. In all experiments NTCs and samples were replicated.
All experiments and sample setups included NTCs (no template controls) and STA NTCs. In all experiments NTCs and samples were replicated.
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