For both SEM and TEM observations, spheroids were processed by standard fixation and embedding procedures. For TEM images, ultrathin sections were examined using a JEM 1010 (Japan) equipped with a CCD camera (SC1000, Gatan, USA). For SEM images, samples were additionally coated with gold and examined using a JSM-5410LV microscope (JEOL, Japan).
Jsm 5410 lv microscope
Manufactured by JEOL
Sourced in Japan, United States
The JSM–5410 LV is a low-vacuum scanning electron microscope (SEM) manufactured by JEOL. It is designed to provide high-resolution imaging of a wide range of samples, including those that are not easily accommodated in a high-vacuum environment. The JSM–5410 LV utilizes a tungsten filament electron source and offers a magnification range from 20x to 300,000x, with a resolution of 3.5 nm at 30 kV. It is equipped with a secondary electron detector and backscattered electron detector for imaging, as well as an optional energy-dispersive X-ray (EDX) spectrometer for elemental analysis.
Automatically generated - may contain errors
7 protocols using jsm 5410 lv microscope
1
Microscopic Imaging of Spheroids
2
Morphological Study of Millipedes from Laos
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New material was collected from northern Laos in 2014 by SP and members of the Animal Systematics Research Unit, Chulalongkorn University. Photographs of live animals were taken in the laboratory using a Nikon 700D digital camera with a Nikon AF-S VR 105mm macro lens. Specimens were preserved in 75% ethanol, and morphological observations made under an Olympus SZX7stereo microscope.
Scanning electron micrographs (SEM ) were taken with a JEOL, JSM–5410 LV microscope, and the material returned to alcohol upon examination. Pictures of one of the gonopods of the holotypes were taken in the laboratory and assembled using the “CellD” automontage software of the Olympus Soft Imaging Solution GmbH package. The key to all species is principally based on the descriptions by Golovatch et al. (2007a , 2007b , 2010, 2011a , 2011b ). The holotypes, as well as most of the paratypes are housed in the Museum of Zoology, Chulalongkorn University (CUMZ ZMUM
The collecting sites were located by GPS using the WGS84 datum.
The carinotaxy formulae in the descriptions follow those in Golovatch et al. (2007a , 2007b ), while body segment counts are after Enghoff et al. (1993) .
Scanning electron micrographs (
The collecting sites were located by GPS using the WGS84 datum.
The carinotaxy formulae in the descriptions follow those in Golovatch et al. (2007a , 2007b ), while body segment counts are after Enghoff et al. (1993) .
3
Larval Morphology Visualization
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Larvae of different stages were dried with hexamethyldisilazane, mounted on entomological minuten pins and glued with epoxy resin on to standard electron microscopy stubs, sputter-coated with gold and studied under a JEOL JSM-5410LVmicroscope.
Larval head capsules were prepared from the gold-coated specimens as well as from uncoated material by macerating in 0.2M aqueous KOH to remove soft tissues, washing in dilute acetic acid, followed by dehydration through to xylene and mounting in Permount®.
Larval head capsules were prepared from the gold-coated specimens as well as from uncoated material by macerating in 0.2M aqueous KOH to remove soft tissues, washing in dilute acetic acid, followed by dehydration through to xylene and mounting in Permount®.
4
Field Observations and Specimen Documentation
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The material was collected during a field trip to southern Laos undertaken in 2013 by SP and members of the Animal Systematics Research Unit, Chulalongkorn University. Live animals were photographed on the spot. Specimens were preserved in 75% ethanol, and morphological investigations were carried out in the laboratory using an Olympus stereomicroscope. Scanning electron micrographs (SEM) of gonopods coated with gold were taken using a JEOL, JSM–5410 LV microscope, returned to alcohol upon examination. Digital images of the specimens were taken in the laboratory and assembled using the “CellD” automontage software of the Olympus Soft Imaging Solution GmbH package. In addition, line drawings of gonopods were also prepared. All material is housed in the Museum of Zoology, Chulalongkorn University (CUMZ), Bangkok, Thailand.
Collecting sites were located by GPS using the WGS84 datum.
In the catalogue sections, D stands for the original description, subsequent descriptive notes or appearance in a key while M for a mere mention.
Collecting sites were located by GPS using the WGS84 datum.
In the catalogue sections, D stands for the original description, subsequent descriptive notes or appearance in a key while M for a mere mention.
5
Taxonomic Study of Limestone Mountain Fauna
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The material was collected from a limestone mountain area in Yunnan, southern China in October 2016. Photographs of live animals were taken in the laboratory using a Nikon 700D digital camera with a Nikon AF-S VR 105 mm macro lens. Specimens were preserved in 75% ethanol, and morphological investigations were carried out in the laboratory with the help of an Olympus SZX7 stereo microscope. Scanning electron micrographs (SEM) were taken with a JEOL, JSM–5410 LV microscope with no metallic coating, and the material returned from stubs to alcohol upon examination. Images of one holotype gonopod were taken in the laboratory and assembled using the “CellD” automontage software of the Olympus Soft Imaging Solution package. The holotype and most of the paratypes are housed in the Museum of Zoology, Chulalongkorn University (CUMZ Zoological Museum, State University of Moscow , Russia (ZMUM
The collecting site was located by GPS using the WGS84 datum.
In the catalogue sections, D stands for the original description and/or subsequent descriptive notes, K for the appearance in a key, L for the appearance in a species list, R for a new subsequent record, and M for a mere mention.
The collecting site was located by GPS using the WGS84 datum.
In the catalogue sections, D stands for the original description and/or subsequent descriptive notes, K for the appearance in a key, L for the appearance in a species list, R for a new subsequent record, and M for a mere mention.
6
Morphological Analysis of Gonopods from Northern Laos
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Material was collected in northern Laos in 2014 by SP and members of the Animal Systematics Research Unit, Chulalongkorn University. Specimens were preserved in 75% ethanol, and morphological investigations were carried out in the laboratory using an Olympus stereomicroscope.
Collecting sites were located by GPS using the WGS84 datum.
In the catalogue sections, D stands for the original description, subsequent descriptive notes or appearance in a key, R for a subsequent record or records, and M for a mere mention.
Scanning electron micrographs
Museum of Zoology, Chulalongkorn University
Collecting sites were located by GPS using the WGS84 datum.
In the catalogue sections, D stands for the original description, subsequent descriptive notes or appearance in a key, R for a subsequent record or records, and M for a mere mention.
7
Electron Microscopy Sample Preparation
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Briefly, the particles were purified and fixed with 2.5% glutaraldehyde in phosphate buffer (0.1 M, pH 7.4). Post-fixation was performed with 1% osmium tetroxide in phosphate buffer. The samples were dehydrated with alcohol gradients and dried to critical point and coated with gold for observation. Finally, the JEOL JSM 5410LV microscope (Jeol USA, USA) was used to observe the samples.
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