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Application suite v 2

Manufactured by Leica

Application Suite V 2.5.0 is a software package designed to support the operation and management of Leica lab equipment. It provides a comprehensive platform for instrument control, data acquisition, and analysis. The software suite is compatible with a range of Leica products and is regularly updated to ensure compatibility and functionality.

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2 protocols using application suite v 2

1

Giemsa Staining of Skin Cell Cytology

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The isolated dorsal and ventral skin cells were adjusted to 105 cell ml-1 and cytocentrifuged (800 g, 5 min, 4°C). Next, the cells were fixed in methanol for 10 min and stained with Giemsa (Merck) at 10% (v/v) for 60 min, washed with tap water and mounted with DPX (Merck). The slides were then examined under a light microscope (Leica DM6000B) and images were obtained with a digital camera (Leica DFC280) and processed by Leica Application Suite V 2.5.0. Software.
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2

Histological Analysis of Skin Samples

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Skin samples (n = 6) were collected and processed as described elsewhere [63 (link)]. Skin samples were sectioned at 5 μm and stained with periodic acid–Schiff (PAS; Merck) according to the manufacturer’s instructions. Images were obtained under a light microscope (Leica DM6000B) with a digital camera (Leica DFC280) and processed by Leica Application Suite V 2.5.0. Software.
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