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3 protocols using human serum

1

Comprehensive Analytical Reagents Protocol

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Tetrabutyl orthotitanate (TBOT), boric acid, ammonium chloride, disodium phosphate, sodium dihydrogen phosphate, methanol, ethanol, acetonitrile, 3,3′,5,5′-tetramethyl benzidine, glucose, fructose, maltose, galactose, lactose, citric acid were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Uric acid, lauric acid, ascorbic acid, L-aspartic acid were purchased from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA), Human serum albumin (HSA), horse radish peroxidase, glucose oxidase, L-cysteine, glutathione, L-phenylalanine, glycine, L-tryptophan, L-methionine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Human serum was purchased from Solarbio (Beijing, China).
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2

Biochemical Reagent Procurement Protocol

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Urea was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). NaCl was purchased from Chongqing Chuandong Chemical Co., Ltd. (Chongqing, China). Sodium bromide and bovine serum albumin were purchased from Titan Scientific Co., Ltd. (Shanghai, China). Sucrose was purchased from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). L-serine hydrochloride, invertase, fibrinogen from bovine plasma, L-glutamic acid monosodium salt, and α-amylase from porcine pancreas were purchased from Yuanye Biological Technology Co., Ltd. (Shanghai, China). (Beijing, China). Thrombin was purchased from Sigma (St Louis, MO, USA). Human serum was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China).
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3

Cytochrome c Binding and Cellular Toxicity

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Cyt c was purchased
from Sigma-Aldrich (USA). Indium(III) chloride
tetrahydrate (InCl3·4H2O) and copper(II)
chloride dihydrate (CuCl2·2H2O) were purchased
from Energy Chemical (Shanghai, China). Myoglobin (Mb), hemoglobin
(Hb), bovine serum albumin (BSA), human serum albumin (HSA), immunoglobulin
G (IgG), lysozyme (Lyz), glutathione (GSH), adenosine triphosphate
(ATP), etoposide, and human serum were purchased from Solarbio (Beijing,
China). Zinc acetate (Zn(Ac)2) was purchased from Meryer
Chemical Technology Co., Ltd. (Shanghai, China). Human cervical cancer
cell line (HeLa) was obtained from the Cell Bank of Type Culture Collection
of the Chinese Academy of Sciences (Shanghai, China). Penicillin,
fetal bovine serum (FBS), streptomycin, and Dulbecco’s modified
Eagle’s medium (DMEM) were purchased from Gibco (Thermo Fisher
Scientific). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) was purchased from Sigma-Aldrich Inc. (Saint Louis,
MO, USA).
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