Cocaine HCl was provided by the National Institute on Drug Abuse (Research Triangle Park, NC). SCH-23390 HCl was purchased from Sigma (St. Louis, MO). Cocaine and SCH-23390 were dissolved in 0.9% sterile saline. The dose of SCH-23390 was chosen based on our previous work (Ball et al., 2017 (link); Ball et al., 2015 (link); Ball et al., 2016 (link)) and the doses of cocaine were chosen based on pilot data. All doses refer to weight of salt.
Sch 23390 hcl
Manufactured by Merck Group
Sourced in Canada
SCH-23390 HCl is a chemical compound used in laboratory research. It is a selective dopamine D1 receptor antagonist. The core function of SCH-23390 HCl is to facilitate the study of dopamine D1 receptor-mediated processes in various experimental models and applications.
Automatically generated - may contain errors
4 protocols using sch 23390 hcl
1
Cocaine and SCH-23390 Dosing Protocol
2
Pharmacological Manipulation of Dopamine Signaling
3
Cocaine Self-Administration Pharmacology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(−)-Cocaine HCl was provided by the Research Triangle Institute (Chapel Hill, NC) under the National Institute on Drug Abuse drug supply program. EEDQ, SCH23390 HCl and S(−)-Eticlopride HCl were purchased from Sigma-Aldrich, St. Louis, MO. Cocaine was dissolved in saline at the concentration of 40 µmol/ml. EEDQ was dissolved in 95% ethanol at the concentration of 2.0 mg/ml immediately or not more than 3 days before the injection. Stock solution of SCH23390 and Eticlopride were dissolved in 95% ethanol at the concentration of 20 μmol/ml, were stored at − 20 °C. Solutions were further diluted before animal injections for a maximum ethanol concentration of 10%. The dose of ethanol injected along with eticlopride or SCH23390 was 0.02 mg/kg and did not affect cocaine self-administration behavior26 (link).
4
Locomotor Behavior in Syt1 cKO Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The locomotor behavior of 11–12-week-old male or female Syt1 cKODA mice was recorded using an infrared actimeter (Superflex sensor version 4.6, Omnitech) using the Fusion software (v5.6 Superflex Edition, RRID:SCR_017972). A chamber partition was used to measure two mice at a time. Subjects were not given time to acclimate and spent a total of 60 min in the chamber with the following protocol: the first 20 min was used to record basal locomotion, while the following 40 min was used to record locomotion after saline or drug administration (i.p.). Drug treatments correspond to a single dose of cocaine hydrochloride at 20 mg/kg (Medisca, cat# 53-21-4, Canada) or D-amphetamine sulfate at 5 mg/kg (Tocris, 2813, UK) at doses known to increase locomotion87 (link),88 (link). SCH23390-HCl (Sigma, D-054, Canada) at 50 µg/kg, quinpirole-HCl at 0.2 mg/kg (Sigma, Q-102, Canada) and raclopride l -tartrate at 1 mg/kg (Sigma, R-121, Canada) were also used at selected high doses known to reduce locomotion in the open field89 (link)–91 (link). Each drug was diluted into 0.9% sodium chloride saline solution (Halyard, #cat 116). For each drug tested, a different cohort of mice was used. Results are presented as the mean of the traveled distance.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!