Il 27

Manufactured by Miltenyi Biotec

Sourced in Germany

IL-27 is a cytokine that belongs to the IL-6/IL-12 family. It is composed of two subunits, EBI3 and p28. IL-27 is involved in the regulation of the immune response and can have both pro-inflammatory and anti-inflammatory effects.

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Lab products found in correlation

3 protocols using il 27

Expansion and Cloning of ILC2 and NK Cells

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Sorted bulk ILC2 and NK cells were plated in 96-well round-bottom plates with irradiated allogeneic PBMCs (45 Gy; combined from 6 healthy donors) and purified phytohemagglutinin (0.2 µg/ml; Oxoid) in Yssel’s medium (Yssel and Spits, 2002 ) supplemented with 2% human AB serum (Establissement Français du Sang). ILC2 and NK clones were obtained after sorting single cells directly into 96-well plates preseeded with irradiated PBMCs or stromal cells lines, OP9 or OP9-DL4. Cytokines IL-2, -7 (5 ng/ml each; Miltenyi Biotec), -12, -18, -25, -33, -1β, -23 (10 ng/ml each; R&D Systems) are provided in various combinations, as indicated. Fresh cytokines and medium were replenished every 4 d. Unless otherwise indicated, bulk cultures were analyzed after 7 d and clones after 14 d.

Lim A.I., Menegatti S., Bustamante J., Le Bourhis L., Allez M., Rogge L., Casanova J.L., Yssel H, & Di Santo J.P. (2016). IL-12 drives functional plasticity of human group 2 innate lymphoid cells. The Journal of Experimental Medicine, 213(4), 569-583.

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Murine and Human Cytokine Isolation

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Murine IL-1β, IL-2, IL-6, IL-7, IL-12, IL-23, IL-27 were obtained from Miltenyi (Bergisch-Gladbach, Germany). Murine IL-4 and Human TGF-β1 were obtained from Biolegend (San Diego, CA, USA).

Nawrocki M., Lory N., Bedke T., Stumme F., Diercks B.P., Guse A.H., Meier C., Gagliani N., Mittrücker H.W, & Huber S. (2021). Trans-Ned 19-Mediated Antagonism of Nicotinic Acid Adenine Nucleotide—Mediated Calcium Signaling Regulates Th17 Cell Plasticity in Mice. Cells, 10(11), 3039.

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Cytokine Stimulation Assay

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Cells were stimulated with indicated concentrations of recombinant human IL-6, OSM, LIF, IFN-α, IL-11, IL-27, and IL-21 (Miltenyi Biotec, R&D Systems, and PeproTech).

Chen Y.H., Grigelioniene G., Newton P.T., Gullander J., Elfving M., Hammarsjö A., Batkovskyte D., Alsaif H.S., Kurdi W.I., Abdulwahab F., Shanmugasundaram V., Devey L., Bacrot S., Brodszki J., Huber C., Hamel B., Gisselsson D., Papadogiannakis N., Jedrycha K., Gürtl-Lackner B., Chagin A.S., Nishimura G., Aschenbrenner D., Alkuraya F.S., Laurence A., Cormier-Daire V, & Uhlig H.H. (2020). Absence of GP130 cytokine receptor signaling causes extended Stüve-Wiedemann syndrome. The Journal of Experimental Medicine, 217(3), e20191306.

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