Dsri2 colour camera

Manufactured by Nikon

The DSRi2 is a high-resolution colour camera designed for scientific and industrial applications. It features a 12-megapixel CMOS sensor with a wide dynamic range and low-noise performance. The camera captures detailed images with accurate colour reproduction and is capable of high-speed image acquisition.

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Lab products found in correlation

Axiocam mrm, by Zeiss (1 mentions) Lsm 710 confocal microscope, by Zeiss (1 mentions) Observer z1 inverted microscope, by Zeiss (1 mentions) Ti e inverted microscope, by Nikon (1 mentions) 5.5 scmos camera, by Oxford Instruments (1 mentions)

2 protocols using dsri2 colour camera

Imaging and Quantification Techniques for Biological Samples

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Digital images were captured on a Nikon TiE inverted microscope (Nikon Instruments), a Zeiss Observer.Z1 inverted microscope (Zeiss) or a Zeiss LSM 710 confocal microscope (Zeiss). The motorized stage and automated image-stitching parameters were used to obtain complete images of each tumour/tissue array. Immunohistochemical stains were captured using brightfield optics and a Nikon DSRi2 colour camera at ×20 magnification. Multi-channel immunofluorescent samples were captured on a CCD Hammamatsu Camera at ×10 magnification and an AxioCam MRm (Zeiss) at ×10 and ×20 magnification, using independently controlled excitation and emission wavelength filter-wheels for each specific fluorophore. NIS Elements software v.4.60.00 (Nikon) and Zeiss Zen 3.0 software (Zeiss) were used for image quantification and pseudo-colouring of immunofluorescent images where appropriate. Also, ImageJ Fiji^{81 (link)} was used for image quantification. Parameters in the tube formation assay were analysed in ImageJ Fiji using a code for the morphometric analysis of ‘Endothelial Tube Formation Assay’^{82 (link)}. Imaris 9.8.1 imaging software was used to analyse images capture by confocal/multiphoton microscope.

Verginadis I.I., Avgousti H., Monslow J., Skoufos G., Chinga F., Kim K., Leli N.M., Karagounis I.V., Bell B.I., Velalopoulou A., Salinas C.S., Wu V.S., Li Y., Ye J., Scott D.A., Osterman A.L., Sengupta A., Weljie A., Huang M., Zhang D., Fan Y., Radaelli E., Tobias J.W., Rambow F., Karras P., Marine J.C., Xu X., Hatzigeorgiou A.G., Ryeom S., Diehl J.A., Fuchs S.Y., Puré E, & Koumenis C. (2022). A stromal Integrated Stress Response activates perivascular cancer-associated fibroblasts to drive angiogenesis and tumour progression. Nature Cell Biology, 24(6), 940-953.

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Fluorescent and Confocal Microscopy Imaging

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Imaging was performed using an Eclipse Ti epifluorescent microscope (Nikon, Tokyo, JPN). For brightfield imaging, the Nikon DS-Ri2 colour camera was used. For fluorescent imaging, a Lumencor Spectra X light engine was utilised to power LED light sources for excitation. Emission filters for DAPI, FITC, TRITC, Cy5, and Li-Cor 740 dyes were used, and fluorescent emissions were detected using an Andor Zyla 5.5 sCMOS camera. Confocal microscopy was performed using an Olympus FV3000RS laser-scanning confocal microscope, utilising OBIS LS/LX laser modules (Coherent, Santa Clara, CA, USA) and FV3000 Spectral Detector and High-sensitivity Spectral Detector units (Olympus, Center Valley, PA, USA). Final image processing was performed using NIS Elements Advanced Research package (Nikon) and ImageJ software (NIH).

Segaliny A.I., Cheng J.L., Farhoodi H.P., Toledano M., Yu C.C., Tierra B., Hildebrand L., Liu L., Liao M.J., Cho J., Liu D., Sun L., Gulsen G., Su M.Y., Sah R.L, & Zhao W. (2019). Combinatorial targeting of cancer bone metastasis using mRNA engineered stem cells. EBioMedicine, 45, 39-57.

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