Laser module

Manufactured by Oxford Instruments

Sourced in Germany

The Laser module is a compact and versatile device that generates a highly collimated and monochromatic beam of light. It serves as a source of coherent optical radiation for various laboratory and research applications.

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Lab products found in correlation

Eclipse ti microscope, by Yokogawa (1 mentions) Du897 emccd camera, by Oxford Instruments (1 mentions) Csu x1 spinning disk, by Agilent Technologies (1 mentions) Ti2 e microscope, by Hamamatsu Photonics (1 mentions) Fusionbt scmos camera, by Hamamatsu Photonics (1 mentions) Mvx10 zoom body, by Olympus (1 mentions) Ultramicroscope 2, by Miltenyi Biotec (1 mentions) Neo scmos camera, by Oxford Instruments (1 mentions)

2 protocols using laser module

Timelapse Imaging of Cell Lines

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Timelapse imaging of NIH3T3 cells, SYF cells, and HEK293T cells was performed on a Nikon Eclipse Ti microscope with a Yokogawa CSU-X1 spinning disk, an Agilent laser module containing 405, 488, 561, and 650 nm lasers, and an iXon DU897 EMCCD camera, using ×40 or ×60 oil objectives. Timelapse imaging of MCF10A cells on polyacrylamide substrata was performed on a Nikon Ti2-E microscope with a CSU-W1 SoRa spinning disk, a Hamamatsu FusionBT sCMOS camera, using a ×20 air objective with ×2.8 magnification optics.

Farahani P.E., Yang X., Mesev E.V., Fomby K.A., Brumbaugh-Reed E.H., Bashor C.J., Nelson C.M, & Toettcher J.E. (2023). pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells. eLife, 12, e82863.

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3D Imaging of Neurovascular Structure

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Cleared and immunostained tissues were imaged with UltraMicroscope II (LaVision BioTec, Bielefeld, Germany) equipped with an Olympus MVX10 zoom body (Olympus, Bartlett, TN), a LaVision BioTec Laser Module, and an Andor Neo sCMOS Camera with a pixel size of 6.5 μm. The following lasers and filters were used: Cy2-AVP was excited at 488 nm and emission was acquired at 525 ± 50 nm; Cy3-SMA was excited at 561 nm and acquired by 605 ± 50 nm; Cy5-collagen was excited at 639 nm and emission was collected at 705 ± 72 nm. The vasculature of the SCN region was scanned horizontally with a voxel size 1.21 μm × 1.21 μm × 1.5 μm (LE-RI × R-C × D-V). Stitching was performed using 3 × 3 1940 μm × 2750 μm × 960 μm stacks with 20% overlap.

Bacigalupo J., Johnson E.C., Vergara C, & Lisman J.E. (1991). Light-dependent channels from excised patches of Limulus ventral photoreceptors are opened by cGMP.. Proceedings of the National Academy of Sciences of the United States of America, 88(18), 7938-7942.

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