Blood samples were collected in Greiner Bio-One Hematology K3EDTA Evacuated Tubes (Thermo Fisher Scientific). Complete blood counts, including leukocyte differentials and reticulocyte counts (CBC/Diff/Retic), were determined using the Procyte DX (IDEXX Laboratories, Westbrook, ME, USA). The Catalyst One analyzer (IDEXX Laboratories) was used for biochemical analyses of serum samples, which were collected in Greiner Bio-One VACUETTE Z Serum Sep Clot Activator Tubes (Thermo Fisher Scientific). Samples were run on both machines the day of collection shortly following collection such that they were not stored prior to analysis. On D30, an equipment issue with the Procyte DX required that samples from the virus-exposed group be stored at 4°C for about 30 h prior to analysis. For a list of all measured parameters and their values, see Supplementary Table 3 .
Catalyst one analyzer
Manufactured by IDEXX
Sourced in United States
The Catalyst One analyzer is a laboratory instrument designed for in-clinic diagnostic testing. It provides rapid and accurate analysis of various parameters in animal samples, such as blood, urine, and other bodily fluids. The Catalyst One analyzer is capable of performing a wide range of diagnostic tests to support veterinary care.
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Lab products found in correlation
5 protocols using catalyst one analyzer
1
Blood Sample Collection and Analysis
2
Blood Sample Collection and Analysis
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At the end of the study, blood samples were collected from the two groups between 19:00 and 19:30. The blood samples were collected in a sterilized tube after cardiac puncture (Kube et al., 2021 (link)). All the samples were placed on ice for 60 min for blood clotting and then centrifuged at 3,000 × g for 10 min, 4°C. The serum samples were then transferred into sterilized tubes for the biochemistry assay. Serum biochemistry profiles, including electrolytes, glucose, amylase, and lipase, were measured using a preloaded Chem 17 CLIP using a Catalyst One analyzer (IDEXX Laboratories, Inc., ME, United States) following the manufacturer’s instructions.
3
Multiparametric Blood Biomarker Analysis
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Blood plasma collected in lithium heparin tubes was used for blood chemistries. The IDEXX Catalyst One Analyzer (IDEXX, Westbrook, Maine, USA) clips included creatinine (CREA), creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate (LAC), and lactate dehydrogenase (LDH). Blood plasma specimens collected in EDTA tubes were evaluated for inflammatory markers. Initially, a 27-plex assay was evaluated to identify detectable analytes to down-select. Next, a customized rat four-plex cytokine/chemokine kit (Millipore, Burlington, MA, USA) on a Bio-plex® 200 Luminex system (Bio-Rad, Hercules, CA, USA) was used for analyses according to the manufacturer’s instructions based on the analytes that had detectable levels from the 27-plex assay. The custom kit included interleukin (IL)-1β, IL-6, inflammatory protein-10 (IP-10), and leptin.
4
Serum Biomarkers in Liver Disease
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Ghrelin was assessed in serum of Mdr2KO and FVBN mice, using an ELISA kit purchased from Phoenix Pharmaceuticals, Inc (Burlingame, CA), according to the manufacturer’s instructions. Serum markers for liver function, ALT and AST, from mice treated with vehicle, Ghr or DG were assessed using the IDEXX Catalyst One analyzer from IDEXX Laboratories (Westbrook, ME). Proinflammatory cytokine CCL2 and profibrogenic growth factor TGFβ1 were assessed using ELISA kits from ThermoFisher (Waltham, MA).
5
Broiler Chicken Blood Analysis
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A day before slaughter, blood samples were collected in the morning by a trained animal health technician from the wing vain of 2 randomly selected birds per pen into purple-top EDTA-coated vacutainer tubes (for haematology analysis) and red-top Vacuette® Serum Clot Activator tubes without EDTA (for sero-biochemistry analysis). Red blood cells, white blood cells, lymphocytes, heterophils, monocytes, eosinophils, basophils, platelets, haemoglobin, and other haematological parameters were analysed using an automated IDEXX LaserCyte Hematology Analyzer (IDEXX Laboratories, Westbrook, USA) whilst sero-biochemical parameters (glucose, symmetric dimethylarginine (SDMA), phosphate, calcium, total protein, albumin, globulin, albumin/globulin, alanine transaminase, alkaline phosphatase, total bilirubin, cholesterol, amylase, and lipase) were analysed using an automated IDEXX Catalyst One Analyzer (IDEXX Laboratories, Westbrook, USA).
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