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Tmt tandem mass tagtm kits

Manufactured by Thermo Fisher Scientific
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Sourced in United States

TMT (Tandem Mass TagTM) kits are a set of isobaric labeling reagents used for quantitative proteomics analysis. The kits allow for simultaneous identification and relative quantification of proteins in multiple samples. The core function of TMT kits is to enable multiplexed peptide/protein identification and quantification using tandem mass spectrometry.

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Lab products found in correlation

Edu kits, by RiboBio (1 mentions) Urea, by GE Healthcare (1 mentions) Sequencing grade trypsin lysc, by Promega (1 mentions) Anti actb gtx124213, by GeneTex (1 mentions) Proteinase inhibitor cocktail, by Roche (1 mentions)

Close spelling variants of this product

TMT Tandem Mass Tag (TMT, TMT kit

2 protocols using tmt tandem mass tagtm kits

1

Comprehensive Protein Analysis Workflow

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The EdU kits were purchased from Guangzhou RiboBio Co., Ltd. Meanwhile, siRNA LIF and Shanghai Jima Biotechnology Co., Ltd. synthesized siRNAs LIF and negative control. TMT (Tandem Mass TagTM) kits were purchased from Thermo Fisher Scientific (NJ, USA). The following antibodies were used for western blotting: anti- STAT3 (Cat. No. 12640, CST), anti-LIF (Cat.No.26757-1-AP), anti-JAK1 (Cat.No.66466-1-Ig) and horseradish peroxidase-conjugated secondary antibodies (Cat. No. 150159) were from Proteintech (Wuhan, China).
Luo H., Yang Z., Zhang Q., Li T., Liu R., Feng S., Liu Z., Sun S., Chen J., Wang X, & Xie X. (2023). LIF Inhibits Proliferation of Esophageal Squamous Carcinoma Cells by Radiation Mediated Through JAK-STAT Signaling Pathway. Journal of Cancer, 14(4), 532-543.
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2

Multiplex Proteomic Analysis of Cellular Processes

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Urea was purchased from GE Healthcare (LC, UK). Proteinase inhibitor cocktail was purchased from Roche (BS, CH). TMT (Tandem Mass TagTM) kits were purchased from Thermo Fisher Scientific (NJ, USA). Sequencing grade Trypsin/Lys-C was purchased from Promega (WI, USA). Antibodies: Anti-ACTB (GTX124213, GeneTex, CA, USA), anti-FN1 (ab32419, Abcam, Cambridge, UK), and anti-NOS3 (sc-376751, Santa Cruz, CA, USA). The other reagents were obtained from Sigma (MO, USA).
Wang X., Liu Y., Jia Y., Liu H., Bao X., He Z, & Ge W. (2019). Proteome Profiling of Cerebral Vessels in Rhesus Macaques: Dysregulation of Antioxidant Activity and Extracellular Matrix Proteins Contributes to Cerebrovascular Aging in Rhesus Macaques. Frontiers in Aging Neuroscience, 11, 293.
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