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2 protocols using phospho camkiv

1

Calcium Signaling Regulation by BK Channels

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Paxilline, IbTx, STO-609, thapsigargin, EGTA, ruthenium red, heparin, digitonin, 2-APB, U0126, bicuculline, DMSO, DAPI and all the other reagents to make solutions were from Sigma-Aldrich. Fluo-4/AM, Fluo-4/dextran, X-Rhod-1 were from Life Technologies. Antibodies specific for BK channel α-subunit (L6/60, Millipore MABN70, immunocytochemisty (ICC) 20 μg/ml, western blot (WB) 10 μg/ml, reference (Ref.) PMID 16566008), BK channel β4-subunit (Alomone Lab APC-061, ICC 1:100, Ref. PMID 21697543), Lamin B (Abcam ab16048, WB 1:1000, ICC 1:500, Ref. PMID 24265311) GAPDH (Cell Signalling 3683S, WB 1:1000, Ref. PMID 22895339), Calnexin (Cell Signalling 2433S, WB 1:1000, Ref. PMID 19815548), COX IV (Cell Signalling 4850S, WB 1:1000, Ref. PMID 19737931), phospho-CREB (Cell Signalling 9198S, WB 1:1000, ICC 1:500, Ref. PMID 17906627), CREB (Cell Signalling 9197S, WB 1:1000, Ref. PMID 20864036), phospho-CaMKIV (Santa Cruz Biotechnology sc-28443-R, WB 1:200, Ref. PMID 18559891), CaMKIV (Cell Signalling 4032S, WB 1:1000, Ref. PMID 21784978), phospho-ERK (Cell Signalling 9101S, WB 1:1000, Ref. PMID 11782377), and ERK (Cell Signalling 9102S, WB 1:1000, Ref. PMID 21669876), actin (Cell Signalling 4967S, WB 1:1000, Ref. PMID 17525163), RyR (Thermo MA3-925, WB 1:1000, Ref. PMID 1645737), Na/K ATPase (Cell Signalling 3010S, WB 1:1000, Ref. PMID 21724843) were used.
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2

Calcium Signaling Regulation by BK Channels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Paxilline, IbTx, STO-609, thapsigargin, EGTA, ruthenium red, heparin, digitonin, 2-APB, U0126, bicuculline, DMSO, DAPI and all the other reagents to make solutions were from Sigma-Aldrich. Fluo-4/AM, Fluo-4/dextran, X-Rhod-1 were from Life Technologies. Antibodies specific for BK channel α-subunit (L6/60, Millipore MABN70, immunocytochemisty (ICC) 20 μg/ml, western blot (WB) 10 μg/ml, reference (Ref.) PMID 16566008), BK channel β4-subunit (Alomone Lab APC-061, ICC 1:100, Ref. PMID 21697543), Lamin B (Abcam ab16048, WB 1:1000, ICC 1:500, Ref. PMID 24265311) GAPDH (Cell Signalling 3683S, WB 1:1000, Ref. PMID 22895339), Calnexin (Cell Signalling 2433S, WB 1:1000, Ref. PMID 19815548), COX IV (Cell Signalling 4850S, WB 1:1000, Ref. PMID 19737931), phospho-CREB (Cell Signalling 9198S, WB 1:1000, ICC 1:500, Ref. PMID 17906627), CREB (Cell Signalling 9197S, WB 1:1000, Ref. PMID 20864036), phospho-CaMKIV (Santa Cruz Biotechnology sc-28443-R, WB 1:200, Ref. PMID 18559891), CaMKIV (Cell Signalling 4032S, WB 1:1000, Ref. PMID 21784978), phospho-ERK (Cell Signalling 9101S, WB 1:1000, Ref. PMID 11782377), and ERK (Cell Signalling 9102S, WB 1:1000, Ref. PMID 21669876), actin (Cell Signalling 4967S, WB 1:1000, Ref. PMID 17525163), RyR (Thermo MA3-925, WB 1:1000, Ref. PMID 1645737), Na/K ATPase (Cell Signalling 3010S, WB 1:1000, Ref. PMID 21724843) were used.
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