Simulated saliva was formulated by dissolving the following in deionized water: 1 g/L of mucin (Type II, Sigma-Aldrich, catalog number M2378, St Louis, MO, U.S.A.), 1.8 g/L of a-amylase (from Bacillus subtilis, MP Biomedicals, catalog number 100447, activity of 160,000 BAU/g, Solon, OH, USA), 0.117 g/L of NaCl, 0.149 g/L of KCl, and 2.10 g/L of NaHCO 3 . The solution was adjusted to pH 7 using 0.01 N NaOH. Simulated gastric juice was formulated by dissolving the following in deionized water: 1.5 g/L of mucin, 8.78 g/L of NaCl, and 1.0 g/L of pepsin (from porcine gastric mucosa, MP Biomedicals, Solon, OH, USA, measured activity of 242 U/mg). The pH of the simulated gastric juice was adjusted to 1.8 using 1 N HCl. Both solutions were prepared without enzymes and stored at 4 C, and the enzymes (a-amylase or pepsin) were added into the solutions immediately before digestion experiments (Mennah-Govela and Bornhorst, 2016a).
Pepsin
Manufactured by MP Biomedicals
Sourced in United States
Pepsin is a proteolytic enzyme derived from porcine stomach. It is commonly used in various laboratory applications for the digestion and hydrolysis of proteins.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Mucin, by Merck Group (1 mentions)
Butylated hydroxyanisole, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
β carotene, by Merck Group (1 mentions)
Ammonium sulphate, by Merck Group (1 mentions)
Pancreatin from porcine pancreas, by Merck Group (1 mentions)
Potassium phosphate, by Merck Group (1 mentions)
Ab81298, by Abcam (1 mentions)
Misoprostol, by Merck Group (1 mentions)
Ab16885, by Abcam (1 mentions)
Collagen 1, by Merck Group (1 mentions)
Anti rabbit 800, by LI COR (1 mentions)
Anti mouse 680, by LI COR (1 mentions)
Anti mouse 800, by LI COR (1 mentions)
Pyk2 tyr 402, by Abcam (1 mentions)
7 protocols using pepsin
1
Simulated Saliva and Gastric Juice Formulation
2
Antioxidant and Preservative Compound Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1,1-Diphenyl-2-picrylhydrazyl (DPPH), bile salt, butylated hydroxyanisole (BHA), β-carotene, α-tocopherol, glycine, ammonium sulphate, and linoleic acid were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Pepsin was purchased from MP Biomedicals (France). Thiobarbituric acid (TBA) was purchased from Suvchem (MH, India). Modified starch (E1422) was provided from Sigma Chemical CO., St Louis, MO. Potassium ferricyanide, trichloroacetic acid (TCA), ferrous chloride, ferrozine, sodium hydroxide, Tween 40, NaCl, NaNO2, and tripolyphosphate (TPP) were of analytical grade.
3
Gastric Stability of Therapeutic Drug
4
Protein Kinase Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco's modified Eagle's medium, Trypsin-EDTA, and Phospho-FAK (Tyr-397) recombinant rabbit monoclonal antibody (31H5L17 1:1000 dilution) were from Thermo Fisher (Waltham, MA). Antibodies to FAK-Tyr-397 (ab81298, 1:1000 dilution), Pyk2-Tyr-402 (ab4800, 1:1000 dilution), Src (ab16885, 1:1000 dilution), and Src-Tyr-419 (ab185617, 1:1000 dilution) were from Abcam (San Francisco, CA). Antibodies to total FAK (Anti-FAK, clone 4.47, 05–537, 1:1000 dilution) were from EMD Millipore, (Temecula, CA), and Pyk2 (3292 s, 1:1000 dilution) was from Cell Signaling Technology (Danvers, MA). Secondary antibodies anti-rabbit 800, anti-mouse 680, and anti-mouse 800 were from LI-COR (Lincoln, NE). Pepsin, >2000 U/mg protein was purchased from MP biomedicals (Irvine, CA). Indomethacin, misoprostol, hydroxyurea, potassium phosphate, sodium hydroxide, pancreatin from porcine pancreas, and collagen I were purchased from Sigma Aldrich (St. Louis, MO). Glacial acetic acid was purchased from Fisher Chemical (Cat# A38S-500). Mini-Ames assay and in vitro ADME (absorption, distribution, metabolism, and excretion) studies were conducted by Pharmaron Inc (Beijing, China). Mouse serum chemistries were performed by the veterinary diagnostic laboratory at Michigan State University (Lansing, MI).
5
Comparative Analysis of Insulin Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were of analytical reagent grade. Hydrochloric acid 35–38%, acetonitrile, sodium hydroxide were purchased from Avantor (Center Valley, PA, USA). Sodium perchlorate, phosphoric acid 85%, trifluoroacetic acid (TFA), ammonium carbonate were purchased from Merck (Darmstadt, Germany). Triethylamine, dithiothreitol (DTT), iodoacetamide (IAA), formic acid, HEPES were purchased from Sigma-Aldrich (Munich, Germany). Endoproteinase Glu-C Protease S. aureus V8 and pepsin were purchased from MP Biomedicals (Santa Ana, California, USA). Pharmaceutical formulations: recombinant human insulin (Humulin S®) and recombinant insulin lispro (Humalog®) were from Eli Lilly (Indianapolis, IN, USA), recombinant insulin lispro (Insulin KP drug product) was from IBA (Warsaw, Poland), recombinant insulin aspart (NovoRapid® Penfill®) and recombinant insulin detemir (Levemir®) were from Novo Nordisk (Bagsværd, Denmark), recombinant insulin glargine (Lantus®) was from Sanofi-Aventis (Frankfurt, Germany).
6
Isolation and Infection of C. sinensis Metacercariae
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Metacercariae of C. sinensis were obtained from naturally infected freshwater fish (Pseudorashora para) in an endemic area in Korea. Fish were ground and digested in artificial gastric juice (0.4% pepsin, pH 1.0; MP Biochemicals Co., Solon, OH, USA). The coarse matter remaining after digestion was filtered using a 1-mm-diameter mesh sieve. The filtrate was washed several times with 0.85% saline. Metacercariae were obtained under a dissecting microscope and placed in PBS containing antibiotics and antimycotics at 4 °C until use. Male FVB/NJ mice were orally infected with approximately 30 C. sinensis metacercariae. The mice were fed an appropriate amount of sterilized commercial diet and water ad libitum without further treatment. The mice were euthanized sequentially after 1 and 3 months.
7
Isolation of Clonorchis sinensis from Pseudorasbora parva
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pseudorasbora parva containing C. sinensis metacercariae were obtained from Wuhu city of Anhui Province, China. The metacercariae were isolated through digestion of the fish with artificial gastric juice including pepsin (MP Biomedicals, Germany) and hydrochloric acid, and collected under a dissecting microscope. New Zealand White rabbits were infected intragastrically twice at an interval of 1 week and were sacrificed 6 weeks after the second infection. The livers were removed and C. sinensis adult worms were recovered from the bile duct. The adult worms were washed with phosphate-buffered saline (PBS) and kept in an −80 °C freezer until use.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!