The CAL27, SCC9, SCC25, and SCC15 cell lines were obtained from ATCC (Manassas, VA, USA) and were authenticated recently. The test for mycoplasma contamination was performed and there was no mycoplasma contamination. CAL27, SCC9, SCC25, and SCC15 cells were respectively grown in Dulbecco’s Modified Eagle Medium (DMEM) and DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Biological Industries, Beit- Haemek Israel) and 1% antibiotics (Gibco, 100 U/ml penicillin and 100 μg/ml streptomycin) and cultured in a humidified incubator at 37 °C and 5% CO2. P.g-LPS (standard) (Invitrogen, USA) was used to treat CAL27, SCC15 cell lines at 20 µg/ml for 6 days.
Scc15
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The SCC15 is a compact benchtop centrifuge designed for general laboratory applications. It features a maximum speed of 15,000 rpm and accommodates rotors for various sample tube sizes. The centrifuge provides controlled temperature and time settings to meet specific research needs. Detailed technical specifications and performance data are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
Scc15, by American Type Culture Collection (21 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (17 mentions)
Cal27, by Thermo Fisher Scientific (15 mentions)
Scc25, by Thermo Fisher Scientific (14 mentions)
Scc25, by American Type Culture Collection (12 mentions)
Dmem f12, by Thermo Fisher Scientific (12 mentions)
Cal27, by American Type Culture Collection (12 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (12 mentions)
Hsc 3, by Thermo Fisher Scientific (5 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Plasmotest mycoplasma contamination detection kit, by InvivoGen (2 mentions)
Hek293t, by Thermo Fisher Scientific (2 mentions)
Bovine pituitary extract, by Thermo Fisher Scientific (2 mentions)
Scc 9, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Tca8113, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Merck Group (1 mentions)
29 protocols using scc15
1
Cell Line Cultivation and P.g-LPS Treatment
2
Culturing Human Oral Squamous Carcinoma Cell Lines
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A total of 6 human OSCC cell lines (UMSCC1 (UM1), SCC15, HSC4, CAL33, HSC3, and HSC6) were maintained at 37°C and 5% CO2 in DMEM/F12 (1:1) (Gibco, Invitrogen Corp) supplemented with 10% FBS and different concentrations of glutamine. The SCC15 and human normal oral keratinocyte (HOK) cell lines were purchased from ATCC. The HSC3 and HSC4 cell lines were gifts provided by YYYY Professor (XXXX University, China). The UM1 cell line was obtained from YYYY Professor (Oral and Maxillofacial Surgery department, XXXX University, China) and HSC‐6 and CAL33 cells were kindly donated by YYYY Professor (XXXX Institute of Dental and Craniofacial Research, USA).
3
Cell Culture Protocol for Oral Cancer Research
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A normal tongue epithelial cell line, the Normal Oral Keratinocyte (NOK) cell line, was kindly gifted by the Hospital of Stomatology at Sun Yat-sen University, and the human TSCC cell lines (SCC-9, SCC-15, SCC-25, CAL-27, and Tca8113) were purchased from the Shanghai Institute of Biochemistry and Cell Biology, Cell Bank/Stem Cell Technology platform (CAS). The NOK cell line was cultured in Oral Keratinocyte Medium-basal (ScienCell, SC-2611-b). The human TSCC cell lines (SCC-9, SCC-15, and SCC-25) and CAL-27 were cultured in a mixture of DMEM (Invitrogen) and Ham's F12 medium.The Tca8113 cell line was grown in RPMI-1640 (Invitrogen). The media listed above was supplemented with 10% fatal bovine serum (FBS) (Gibco), 100 μg/mL penicillin/streptomycin (Gibco) and other needed nutrients. All cells were cultured in a humidified incubator with 5% CO2 at 37°C.
4
OSCC Cell Lines and Transfection
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Five human OSCC cell lines (Tca8113, Cal27, SCC-9, SCC-15, and SCC-25) were purchased from American Type Culture Collection (ATCC; Manassas, VA) and cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Carlsbad, CA). Human normal oral keratinocytes (hNOKs; ScienCell Research Laboratories, Carlsbad, CA) were maintained in oral keratinocyte medium (ScienCell Research Laboratories). Human umbilical vein endothelial cells (HUVECs; ATCC) were cultivated in M200 medium (Cascade Biologics, Portland, OR). All cell lines were supplemented with 10% fetal bovine serum (FBS; Gibco BRL), 100 U/ ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, St. Louis, MO) in a humidified atmosphere with 5% CO 2 at 37°C. The small interfering RNA-targeting Nrf2 (siNrf2), HO-1 (siHO-1), and the negative control siRNA (siNC) were synthesized by GenePharma (Shanghai, China) and transfected into SCC-9 and SCC-15 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) in accordance with the manufacturer's
5
Acquisition and Maintenance of HNSCC Cell Lines
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Human HNSCC cell lines SCC9, SCC15, SCC25, CAL27 and HEK293T cells were obtained from ATCC (Rockville, MD, USA). UM1 and UM-SCC1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA). HSC3, HSC6 and CAL33 were kindly provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA). The characteristics of HNSCC cell lines are described in Table S1 . The UM-SCC1, HSC3, HSC6, CAL27, CAL33 and HEK293T cells were cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS, Gibco). The SCC9, SCC15, SCC25 and UM1 cells were maintained in DMEM-F12 (Gibco) supplemented with 10% FBS. All cells were incubated at 37°C in a humidified atmosphere containing 5% CO2. All cell lines were routinely tested for Mycoplasma by PlasmoTestTM Mycoplasma contamination detection kit (InvivoGen).
6
Culturing Oral Cancer Cell Lines
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Human normal oral epithelial cells (HNOECs), SCC-15, sCC-25, and CAL-27 cells were cultured in complete medium (DMED [Gibco]) containing 10% fetal bovine serum (FBS, Invitrogen, California, USA), penicillin, and streptomycin (Solarbio, Beijing, China) at 37°C in 5% CO2, 95% air. HNOECs were purchased from Guide Chem (https://china.guidechem.com , Zhejiang, China) and SCC-15, sCC-25, and CAL-27 cells were obtained from ATCC (www.atcc.org , USA).
7
Culturing OSCC Cell Lines
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OSCC cell lines SCC15 and SCC25 were bought from American Type Culture Collection (ATCC, Manassas, VA, USA). HaCat cells were obtained from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China). Dulbecco's modified Eagle's medium/F12 (Gibco, Carlsbad, CA, USA) was used to culture SCC15 and SCC25 cell lines and Dulbecco’s modified Eagle’s medium (Gibco) was used to culture the HaCat cell line. Fetal bovine serum (10%, Gibco), 1,000 U/ml penicillin and 500 μg/ml streptomycin were added into all cell culture media, and cells were maintained in a humidifying incubator with 5% CO2 at 37°C.
8
Culturing Human Oral Cancer Cell Lines
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Human OSCC cell lines SCC9, SCC15, and CAL27 were obtained from ATCC (Rockville, MD, USA). UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA). HSC3, HSC6 and CAL33 and normal oral keratinocytes (NOK) were kindly provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA). The HSC3, HSC6, CAL27, and CAL33 cells were cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA). The SCC9, SCC15, and UM1 cells were maintained in DMEM-F12 (Gibco) supplemented with 10% FBS. NOK was grown in keratinocyte serum-free medium containing human recombinant epidermal growth factor and bovine pituitary extract (Life Technologies). All cells were incubated at 37°C in a humidified atmosphere containing 5% CO2.
9
Oral Cell Lines for Biological Assays
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A primary normal human gingival fibroblast-1 (HGF-1) cells (CRL-2014, ATCC, VA, USA) was used as a normal oral cell line at 5–10 passages [42 (link)]. HSC-2 (JCRB0622, JCRB Cell Bank, Japan) and SCC-15 (CRL-1623, American type culture collection) were chosen as oral squamous cell carcinoma cell lines. The cells (1 × 104 cells/100 μl) were prepared from 90% confluent cultures biological experiments and seeded in each well of 96 wells (SPL, Pocheon, Gyeonggi-do, Korea). All biological investigations used 96 wells and employed the appropriate cell culture media (DMEM for HGF-1, DMEM/F-12(3:1) for HSC-2 and DMEM/F-12(1:1) for SCC-15) supplemented with 10% fetal bovine serum (Gibco, NY, USA) and 1% antibiotics (penicillin/streptomycin, Gibco). The cells were grown in an incubator at 37°C in a humidified atmosphere containing 5% CO2.
10
Nicotine Treatment of OSCC SCC15 Cells
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The human OSCC cell line SCC15 was purchased from the American Type Culture Collection. SCC15 cells were cultured in a 1:1 mixture of Dulbecco's modified Eagle medium and Nutrient Mixture F-12 medium + 15% fetal bovine serum (FBS) (Gibco, USA) in a 37°C and 5% CO2 environment. The transfected and non-transfected SCC15 cells (1 × 106) were treated with 1 μmol/L nicotine for 7 days.
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