Mouse alveolar macrophages MH‐S cells were acquired from Procell (catalogue number: CL‐0597, Wuhan, China), and grown in MH‐S cell specific media (catalogue number: CM‐0597, Procell) in an incubator at 37°C with 5% carbon dioxide (CO2). MH‐S cells were administrated with 10 μg/mL LPS for 4 h to evoke lung injury in vitro based on the previous report.
30 (link) Small interfering ribonucleic acid (siRNA) targeting ANGPTL2 (si ANGPTL2) and TREM2 (si TREM2), as well as the corresponding negative control (si NC) were prepared by GenePharma (Shanghai, China). The sequences of LILRB2 were sub‐cloned into pcDNA vector plasmids for the overexpression of LILRB2. The transfection was executed on MH‐S cells using Lipofectamine 3000 (catalogue number: L3000075, Invitrogen, Carlsbad, CA, USA) based on the previous description.
31 Cells were harvested for the subsequent assays after 48 h of transfection. Besides, to explore the role of autophagy, MH‐S cells were incubated with rapamycin (an autophagy activator) (catalogue number: IR0010, Solarbio) with a concentration of 3 μM for 12 h based on the previous study.
32 (link)
30 (link) Small interfering ribonucleic acid (siRNA) targeting ANGPTL2 (si ANGPTL2) and TREM2 (si TREM2), as well as the corresponding negative control (si NC) were prepared by GenePharma (Shanghai, China). The sequences of LILRB2 were sub‐cloned into pcDNA vector plasmids for the overexpression of LILRB2. The transfection was executed on MH‐S cells using Lipofectamine 3000 (catalogue number: L3000075, Invitrogen, Carlsbad, CA, USA) based on the previous description.
31 Cells were harvested for the subsequent assays after 48 h of transfection. Besides, to explore the role of autophagy, MH‐S cells were incubated with rapamycin (an autophagy activator) (catalogue number: IR0010, Solarbio) with a concentration of 3 μM for 12 h based on the previous study.
32 (link)