The weight of rats was measured weekly, and the blood sugar was measured by blood glucose meter (J & J). Serum creatinine and blood urea nitrogen (Nanjing Jiancheng Bioengineering Institute) were determined by biochemical kit according to the introduction.
Blood urea nitrogen
Manufactured by Nanjing Jiancheng
Sourced in China
Blood urea nitrogen is a laboratory test that measures the amount of urea nitrogen in the blood. Urea nitrogen is a waste product that is formed when proteins are broken down in the body. This test is used to evaluate kidney function.
Automatically generated - may contain errors
Lab products found in correlation
Beyoecl star, by Beyotime (1 mentions)
Total cholesterol, by Nanjing Jiancheng (1 mentions)
Malonaldehyde mda, by Nanjing Jiancheng (1 mentions)
Catalase cat, by Nanjing Jiancheng (1 mentions)
Glutathione peroxidase gsh px, by Nanjing Jiancheng (1 mentions)
D galactose, by Solarbio (1 mentions)
Malondialdehyde, by Nanjing Jiancheng (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Rnase a, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Thermo Fisher Scientific (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
Guava nexin reagent, by Merck Group (1 mentions)
Pifithrin α, by Merck Group (1 mentions)
Alanine aminotransferase, by Nanjing Jiancheng (1 mentions)
Aspartate aminotransferase, by Nanjing Jiancheng (1 mentions)
5 protocols using blood urea nitrogen
1
Monitoring Metabolic Biomarkers in Rats
2
Antioxidant and Apoptosis Assays
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Uric acid (UA, C012-2-1), Creatinine (CRE, C011-2-1), Blood Urea Nitrogen (BUN, C013-2-1), Reduced glutathione (GSH, A006-2-1) and Malondialdehyde (MDA, A003-1-2) assay kits were from Jiancheng Bioengineering Institute (Nanjing, China). Anhydrous CdCl2 (purity: >99.95%) was from Aladdin Industrial Corporation. Que (purity: > 97%) was from RHAWN (Shanghai, China). RNA isolater Total RNA Extraction Reagent (R401-01), HiScript III RT SuperMix for qPCR (+gDNA wiper, R323-01), and ChamQ universal SYBR qPCR Master Mix (Q711-02) were from novozan Biotechnology (Nanjing, China). β-Actin and antibodies against Caspase-3, Caspase-9 and Cyt-c were from Servicebio (Wuhan, China). BeyoECL Star was from Beyotime Biotechnology (P0018AM, Shanghai, China). All other routine chemicals and solvents were of pure analytical grade.
3
Biochemical Markers in D-Galactose-Induced Aging
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All the kits including liver glycogen (LG), blood lactate acid (Fernández‐Tomé et al.), blood urea nitrogen (BUN), superoxide dismutase (SOD) (No.A‐005), malonaldehyde (MDA) (No.A003‐1), total cholesterol (TC) (No.A111‐1), glutathione peroxidase (GSH‐Px) (No.A005), catalase (CAT) (No.A007‐1), and total cholinesterase (AChE) (No.A024) were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. D‐Galactose was obtained from Solarbio Technology Co. Ltd (Beijing, China). All other chemicals were of analytical grade.
4
Resveratrol's Metabolic Effects
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Resveratrol (purity ≥ 99%) was purchased from Shanghai McLean Company. Blood urea nitrogen (BUN), creatine kinase (CK), total superoxide dismutase (T-SOD), malondialdehyde (MDA) ATPase, succinate dehydrogenase (SDH), cytochrome-c oxidase (COX), and citrate synthase (CS) activities assay kits were purchased from Nanjing Jiancheng Bioengineering Institute. TRIzol reagent was purchased from Ambion Company, the ReverTra Ace qPCR RT Kit was purchased from TOYOBO Company (Osaka, Japan), and the SYBR Green Master (Rox) kit was purchased from Roche Company (Indianapolis, IN, USA).
5
Gypenosides and 5-Fluorouracil Cancer Treatment
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Gypenosides (Gyp) was kindly provided by Ankang Pharmaceutical Institute of the Beijing University (Shaanxi, China) and dissolved in 80% ethanol (EtOH) to a final storage concentration of 100 mg/ml. 5-Fluorouracil (5-Fu) was purchased from Sigam-Aldrich (St. Louis, Mo, USA) and dissolved in dimethyl sulfoxide (DMSO) also to a final storage concentration of 100 mg/ml. Gyp and 5-Fu solution were sterilized through 0.22μm filter for use in subsequent experiments and stored in -20°C.
3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltertrazolium bromide tetrazolium (MTT), Hoechst 33342, propidium iodide (PI), RNase A, N-acetylcysteine (NAC), and pifithrin-α were purchased from the Sigma-Aldrich. Guava Nexin Reagent was obtained from Millipore Corporation (Billerica, MA, USA). 2’, 7’-dichlorofluorescein-diacetate (DCFH-DA) was from Molecular Probes Inc. (Eugene, OR, USA). The aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and serum creatinine (Cr) assay kit were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltertrazolium bromide tetrazolium (MTT), Hoechst 33342, propidium iodide (PI), RNase A, N-acetylcysteine (NAC), and pifithrin-α were purchased from the Sigma-Aldrich. Guava Nexin Reagent was obtained from Millipore Corporation (Billerica, MA, USA). 2’, 7’-dichlorofluorescein-diacetate (DCFH-DA) was from Molecular Probes Inc. (Eugene, OR, USA). The aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and serum creatinine (Cr) assay kit were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
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