Hepa1 6 hepatoma cells

3T3-L1 adipocytes were purchased from the Cell Resource Center of Shanghai Academy of Life Sciences, Chinese Academy of Sciences, while C2-C12 myoblasts and HEPA1-6 hepatoma cells were purchased from ATCC cell bank. 3T3-L1 adipocytes, C2-C12 myoblasts, and HEPA1-6 hepatoma cells were cultured in a DMEM medium containing 10%FBS in an incubator at 37°C and 5% CO2. When the cells grew to the logarithmic phase and the density was 80%, they could be used in the experiment. The above three kinds of cells were transferred to a six-well plate and cultured to about 60% of the cell density, and the lentivirus prepared in advance for knocking out sidt2 and having puro resistance was added to the culture medium. In order to make the lentivirus better-transfected cells, the cells were not treated within 48 hours, and the culture medium could be replaced after 48 hours. 72 hours after the virus was added, the new medium was replaced, and an appropriate amount of puro was added to it to screen cells (the amount of puros varied according to different cell lines). At the same time, it was necessary to add polybrene, to increase the transfection efficiency of the virus. The cells were screened by adding puro for three consecutive days, and the screened cells were cultured normally.