Tris hcl

Manufactured by Sinopharm

Sourced in China

Tris-HCl is a buffer solution used in various laboratory applications. It is a mixture of tris(hydroxymethyl)aminomethane (Tris) and hydrochloric acid (HCl), which provides a stable pH environment for biological and biochemical processes.

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Lab products found in correlation

Sodium chloride (nacl), by Sinopharm (7 mentions) Potassium chloride (kcl), by Sinopharm (4 mentions) Sodium hydroxide (naoh), by Sinopharm (4 mentions) Kh2po4, by Sinopharm (3 mentions) Na2hpo4 12h2o, by Sinopharm (2 mentions) Cocl2, by Sinopharm (2 mentions) Perchloric acid, by Sinopharm (2 mentions) Mgcl2, by Sinopharm (2 mentions) Hydrochloric acid (hcl), by Sinopharm (2 mentions) N n n n tetramethylethylenediamine, by Sinopharm (1 mentions) Dithiothreitol (dtt), by Sinopharm (1 mentions) Rnase h, by Thermo Fisher Scientific (1 mentions) M mlv, by Thermo Fisher Scientific (1 mentions) Amv reverse transcriptase, by Promega (1 mentions) Hepes, by Avantor (1 mentions) T7 rna polymerase, by Promega (1 mentions) 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions) Polygalacturonic acid pga, by Merck Group (1 mentions) Cucl2, by Sinopharm (1 mentions) Mnso4, by Sinopharm (1 mentions)

Spelling variants of this product

Tris(hydroxymethyl)aminomethane hydrochloride (Tris–HCl) (2 citations)

12 protocols using tris hcl

Characterization of Shrimp Spoilage Bacteria

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The shrimp (L. vannamei) was bought from Qidong Rd. Market, Qingdao, with a weight of 45 ± 5 g. The shrimp was transported to the laboratory on ice within 30 min.
The wild strain of L. plantarum SS-128 and the luxS-mutant strain of L. plantarum SS-128 (ΔluxS) were constructed and preserved by the Laboratory of Aquatic Products Higher Application Technology, Ocean University of China. Shewanella baltica is a laboratory-preserved strain previously isolated from spoiled L. vannamei (Zhu et al., 2015 (link)). DPD was obtained from Professor Ming Li’s lab in the College of Pharmacy, Ocean University of China. The De Man, Rogosa and Sharpe (MRS) broth, Lactobacillus medium, and agar were bought from Qingdao Haibo Biotechnology Co., Ltd., China. Na₂HPO₄⋅12H₂O, NaH₂POx⋅H₂O, NaCl, 40% Acr-Bis, Tris–HCl, N,N,N′,N′-tetramethylethylenediamine, Tris, dithiothreitol, and other analytical pure chemical reagents were purchased from Sinopharm Group Chemical Reagent Co., Ltd.

Li Y., Qian Y., Lou X., Hu Z., Hu Y., Zeng M, & Liu Z. (2022). LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach. Frontiers in Microbiology, 13, 892788.

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Enzymatic Assay for Peroxidase Activity

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Hemin (Acrose), HEPES (Amresco), dimethyl sulfoxide (DMSO; Sinopharm Chemical Reagent Co., Ltd.), ABTS [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt; Sigma-Aldrich], H₂O₂ (Sinopharm), Tris-HCl (Sinopharm), KCl (Sinopharm), NaCl (Sinopharm), T7 RNA Polymerase (Promega), AMV Reverse transcriptase (Promega), RNase H (Thermo Scientific), BAS (TaKaRa), M-MLV (Invitrogen). For the hemin solution, hemin was dissolved in DMSO to obtain a final concentration of 10 mM. For the ABTS solution, ABTS was dissolved in DMSO to obtain a final concentration of 250 mM.

Lu X., Shi X., Wu G., Wu T., Qin R, & Wang Y. (2017). Visual detection and differentiation of Classic Swine Fever Virus strains using nucleic acid sequence-based amplification (NASBA) and G-quadruplex DNAzyme assay. Scientific Reports, 7, 44211.

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Expression of Pectate Lyase PEL3 in E. coli

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Escherichia coli Rosetta (DE3)/pET28a-pel3 for the expression of pectate lyase PEL3 was constructed in our previous work (Wang et al., 2018 (link)). Glycine with purity 98.5% and polygalacturonic acid (PGA) with purity 90% were purchased from Sigma-Aldrich (St. Louis, MO, United States). CuCl₂, ZnSO₄, MnSO₄, CoCl₂, CaCl₂, KCl, NaCl, Na₂HPO₄, KH₂PO₄, Tris–HCl, and NaOH were purchased from Sinopharm Chemical Reagent Co., Ltd. All above chemicals were analytical grade. PBS buffer (10 mM) was prepared as follow: 4.00 g of NaCl, 0.10 g of KCl, 0.72 g of Na₂HPO₄, and 0.12 g of KH₂PO₄ were dissolved in 500 mL deionized water.

Wu P., Luo F., Lu Z., Zhan Z, & Zhang G. (2020). Improving the Catalytic Performance of Pectate Lyase Through Pectate Lyase/Cu3(PO4)2 Hybrid Nanoflowers as an Immobilized Enzyme. Frontiers in Bioengineering and Biotechnology, 8, 280.

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Porphyrin and Oligonucleotide Protocol

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5,10,15,20-tetrakis(N-methyl-4-pyridyl)porphyrin (TMPyP) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The oligonucleotides (ODNs), such as polyadenine dA₁₃, polycytosine dC₁₃, polyguanine dG₆TG₆ and polythymine dT₅, dT₁₀, dT₁₃, dT₁₅, dT₂₀, dT₃₀, dT₄₀, dT₅₀ were synthesized by Sangon Biotechnology Co., Ltd. (Shanghai, China). Hg(NO₃)₂, Cd(NO₃)₂·4H₂O, Cr(NO₃)₃·9H₂O, Cu(NO₃)₂·3H₂O, Fe(NO₃)₃·9H₂O, FeSO₄·7H₂O, Mn(CH₃COO)₂·4H₂O, MgCl₂·6H₂O, NiCl₂·6H₂O, Pb(NO₃)₂, ZnCl₂, Tris-HCl, CH₃COOH, NaCl and NaOH were acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the ODNs were prepared with Tris-HCl buffer (10 mM Tris, 100 mM NaCl, pH 7.4). All reagents were of analytical grade and used as received. Ultrapure water was obtained from a Millipore water purification system (≥18 MΩ, Milli-Q, Millipore).

Wu D., Huang Y., Hu S., Yi X, & Wang J. (2018). Sensitive Hg2+ Sensing via Quenching the Fluorescence of the Complex between Polythymine and 5,10,15,20-tetrakis(N-methyl-4-pyridyl) Porphyrin (TMPyP). Sensors (Basel, Switzerland), 18(11), 3998.

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Analytical-grade Reagents and Media

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Analytical grade Na₂SeO₃ (≥99.0%), HCl (≥37%), Glucose anhydrous (≥99.0%), Sucrose anhydrous (≥99.0%), NaCl (≥99.0%), KCl, MgSO₄×7H₂O (≥98.0%), TrisHCl (≥99.0%), NaOH (≥98.0%), Na₂S(≥99.0%), Na₂HPO₄×12H₂O (≥99.0%) and KH₂PO₄ (≥99.5%), were purchased from Sinopharm Chemical Reagent Co. Ltd, China. Biological grade peptone was acquired from Beijing Aoboxing Biotechnology Co. Ltd. Biological grade agar was produced by the Beijing Kangbeisi Technology Co. Ltd. Yeast Extract LP0021 was from Oxoid, made in France. Potassium persulfate (≥99.0%) and 2,2-Diphenyle-1-picrylhydrazyl (DPPH, ≥95.0%) were purchased from Sigma-Aldrich.

Ullah A., Yin X., Wang F., Xu B., Mirani Z.A., Xu B., Chan M.W., Ali A., Usman M., Ali N, & Naveed M. (2021). Biosynthesis of Selenium Nanoparticles (via Bacillus subtilis BSN313), and Their Isolation, Characterization, and Bioactivities. Molecules, 26(18), 5559.

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Degradation Evaluation of MCS-15 in Tris-HCl Buffer

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The degradation property of MCS-15 was evaluated at 37°C in 0.05 M Tris(hydroxymethyl) aminomethane-HCl buffer solution (pH 7.4) (Tris-HCl; Sinopharm Chemical Reagent Co., Ltd.,). The MCS-15 was immersed in the buffer solution in the vials. The vials were then loaded onto an orbital shaker with the speed set at 120 rpm. At the scheduled time, the MCS-15 was separated from the solution by centrifugation, washed, and dried at 120°C to a constant weight. The relative weight loss percentage of the samples was calculated by:

Weight loss (%) = [(W_{0} - W_{t}) / W_{0}] \times 100 %

where W₀ and W_t are the initial weight and the weight after a specific immersion time period t, respectively.

Song W., Li X., Qian J., Lv G., Yan Y., Su J, & Wei J. (2015). Mesoporous calcium–silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2. International Journal of Nanomedicine, 10, 1715-1726.

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Graphene Oxide-based Antibiotic Detection

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Antibiotics, namely FF, FFA, chloramphenicol (CAP), and thiamphenicol (TAP), were purchased from Shanghai Yuanye Technology Biology Co., Ltd. (Shanghai, China). Graphene oxide (GO) was purchased from Guangzhou Wenrui Scientific Instrument Co., Ltd. (Guangzhou, China). Single-stranded DNA having 43 random nucleotides as the central sequence (5′-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3′) and their primers, namely 5′-GCTGTGTGACTCCTGCAA-3′ as the forward primer and 5′-phosphate-GGAGACAAGATACAGCTGC-3′ as the 5′phosphorylated reverse one, were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) [29 (link)]. Tosyl magnetic beads were purchased from Biomag Biotech Co., Ltd. (Wuxi, China). All PCR materials (PCR buffer, ddH₂O, and Mix) were ordered from Kangwei Century Biotech Co., Ltd. (Taizhou, China). Other chemicals, such as KCl, NaCl, and Tris-HCl, were purchased from Sinopharm Chemical Regent Co., Ltd. (Shanghai, China).

Shi M., Liu R., Zhang F., Chitrakar B, & Wang X. (2022). Screening of Single-Stranded DNA Aptamer Specific for Florfenicol and Application in Detection of Food Safety. Biosensors, 12(9), 701.

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Quantification of Amino Acids and Biogenic Amines in Soybeans

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Soybeans were from Heilongjiang Province, China. The standards of 17 FAAs (His, Tyr, Arg, Val, Met, Phe, Ile, Leu, Ser, Gly, Ala, Thr, Lys, Asp, Glu, Cys, Pro) and eight BAs (putrescine, cadaverine, tyramine, spermidine, spermine, histamine, and 2-phenylethylamine), tetrahydrofuran, triethylamine, thrombin, and fibrinogen were bought from Solarbio (Beijing, China). Hydrochloric acid, sodium acetate, trichloroacetic acid, sodium bicarbonate, dansyl chloride, perchloric acid, sodium hydroxide, Tris-HCl, and ammonia were procured from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. All the above reagents were of analytical grade. 2-Methyl-3-heptanone was of chromatographic grade and bought from Solarbio (Beijing, China). Methanol and acetonitrile were of chromatographic grade and purchased from Beijing Bailingwei Technology Co., Ltd., Beijing, China.

Zhang Q., Lan G., Tian X., He L., Li C., Tao H., Zeng X, & Wang X. (2022). Effect of Adding Bifidobacterium animalis BZ25 on the Flavor, Functional Components and Biogenic Amines of Natto by Bacillus subtilis GUTU09. Foods, 11(17), 2674.

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Osteogenic and Angiogenic Biomaterial Synthesis

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Polyvinyl alcohol, (NH₄)₂HPO₄ (99%), Ca(NO₃)₂•4H₂O (99%), Li3PO4 (99%), CoCl₂ (99%), NH3•H2O (25–28%), and Tris–HCl were purchased from Sinopharm Chemical Reagent Co., Ltd. Simulated body fluid (SBF) was purchased from Leagene (China). Minimum Eagle's medium (MEM), Dulbecco’s modified Eagle’s medium/Ham’s F-12 (DMEM/F-12), and fetal bovine serum (FBS) were purchased from HyClone (USA). Endothelial cell medium was obtained from ScienCell (USA). The NBT/BCIP staining kit and detection kit for ALP activity and calcium content were purchased from Beyotime Company (China). The CCK-8 assay was obtained from Dojindo (Japan). MTT, phalloidin, and 4',6-diamidino-2-phenylindole (DAPI) were purchased from AAT Bioquest (USA). Primary antibodies against ALP, BMP-2, RUNX2, OCN, HIF-1α, VEGF, GAPDH, and HRP-conjugated secondary antibodies were obtained from Affinity (China).

Li J., Zhao C., Liu C., Wang Z., Ling Z., Lin B., Tan B., Zhou L., Chen Y., Liu D., Zou X, & Liu W. (2021). Cobalt-doped bioceramic scaffolds fabricated by 3D printing show enhanced osteogenic and angiogenic properties for bone repair. BioMedical Engineering OnLine, 20, 70.

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Seamless Cloning Protocol for Plasmids

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BM Seamless Cloning Kit (Biomed, Beijing, China), DH5 α and Agrobacterium GV3101(Biomed, Beijing, China), small plasmid extraction kit (TIANGEN, Sichuan, China), Gel recovery, DL2000 Marker, High fidelity enzyme and LA Taq (TaKaRa, Beijing, China), X-gal and IPTG (Real Times, Shenzhen, China), Bis, Tris Hcl, SDS, TEMED, ammonium persulfate, ammonium persulfate, kana, sucrose, and agar powder (Sinopharm, Beijing, China), and LB medium (ShengGong, Liaoning, China).

Cai J., Jia R., Jiang Y., Fu J., Dong T., Deng J, & Zhang L. (2023). Functional verification of the JmLFY gene associated with the flowering of Juglans mandshurica Maxim.. PeerJ, 11, e14938.

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