P62 nbp1 49956

The mRNA expression was quantified by real-time RT-PCR using the TaqMan probes (Life Technologies, Carlsbad, CA, USA) in an Applied Biosystems Quantstudio 3 Real-Time PCR System (Foster City, CA, USA). Data were normalized to 18S rRNA. Western blot images were detected with the use of the LI-COR Odyssey scanner and the Image studio 3.1 software (Li-Cor Biosciences, Lincoln, NE, USA). The antibodies used were β-actin (sc-47778) and PEPCK (sc-32879) from Santa Cruz Biotechnology (Dallas, TX, USA); LC3B (ab51520) and Laminin Receptor (ab133645) from Abcam (Cambridge, MA, USA); p62 (NBP1–49956) from Novus Biologicals (Littleton, CO, USA); ATG3 (#3415) and GR (#12041) from Cell Signaling Technology (Danvers, MA, USA); SIRT1 (#07–131) antibody was purchased from Millipore; 11βHSD1 (#10004303) from Cayman chemical (Ann Arbor, MI, USA). The monoclonal antibody against human G6Pase-α has been previously described (Cho et al. 2017 (link)).

The mRNA expression was quantified by real-time RT-PCR using the TaqMan probes (Life Technologies, Carlsbad, CA, USA) in an Applied Biosystems QuantStudio 3 Real-Time PCR System (Foster City, CA, USA). Data were normalized to Rpl19 RNA or 18S rRNA. Western-blots were detected using the LI-COR Odyssey scanner (Li-Cor Biosciences, Lincoln, NE, USA). The antibodies used were β-actin (sc-47778) from Santa Cruz Biotechnology (Dallas, TX, USA); PKM2 (ab137791) from Abcam (Cambridge, MA, USA); p62 (NBP1–49956) from Novus Biologicals(Littleton, CO, USA); p-p62-S349 (#95697), Nrf2 (#12721), p-mTOR-S2448 (#5536), mTOR (#2983), p-4EBP-T37/46 (#2855), 4EBP (#9644), Dvl3 (#3218), non-phospho (active) β-catenin-S33/S37/T41 (#8814), YAP (#12395), p-YAP-S127 (#13008) from Cell Signaling Technology (Danvers, MA, USA).