Heat inactivated low endotoxin fetal bovine serum

Dulbecco’s Modified Eagle Medium (DMEM), heat-inactivated low-endotoxin fetal bovine serum (FBS), and gentamicin purchased from Cambrex (Walkersville MD, USA) for tissue culture. All other cancer cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). Most of these following compounds (thiostrepton, ampicillin, dexamethasone, 2-methoxyestradiol, (−) riboflavin, ascorbic acid, amiloride-HCL, (−) Corey lactone, and quinine sulphate) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA), and quercetin was purchased from Alfa Aesar (Johnson Matthey Co. Lancastor, UK). The 50% purified δ-tocotrienol fraction from annatto seeds was purchased from American River (Boston, MA, USA). RNeasy mini kit was purchased from QIAGEN Sciences (Germantown, MD, USA). DeltaGold (125 mg soft gels) from annatto seeds (composition 90% δ-tocotrienol + 10% γ-tocotrienol) was obtained as a gift by American River Nutrition, Inc. (Hadley, MA. USA). The hepatitis C antibody test kit was purchased from Sigma Chemical Co., St. Louis, USA. The second kit for diagnosing hepatitis C test is RNA (PCR) test. Pure total mRNA was obtained from the EDTA treated fresh whole blood using total RNA purification kit # 17200 (NORGEN Bioteck Corporation, Thorold, ON, Canada).

Deep rough chemotype LPS (Re-LPS) from E. coli D31m4 was purified as described by Qureshi et al. [69 (link)]. For tissue culture studies, RPMI 1640 Medium, heat-inactivated low-endotoxin fetal bovine serum (FBS), and gentamycin were purchased from Cambrex (Walkersville, MD, USA). RNeasy one-step kit was purchased from QIAGEN sciences (Germantown, MD, USA). Highly purified (Affinity column purified and stated by the company to have undetectable LPS, soybean lectin LEC from Glycine max) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Trans-resveratrol (RES >99% HPLC pure) was also purchased from Sigma-Aldrich (St. Louis, MO, USA). PBMCs of normal healthy individual was purchased from Precision for Medicine (Frederick, MD, USA). Male healthy control was a 60-year-old, Caucasian (50 million cells).

Deep rough chemotype LPS (Re-LPS) from E. coli D31m4 was purified as described by Qureshi et al. [74 (link),75 (link),76 (link)]. For tissue culture studies, RPMI 1640 Medium, heat-inactivated low-endotoxin fetal bovine serum (FBS), and gentamycin were purchased from Cambrex (Walkersville, MD, USA). RNeasy one-step kit was purchased from QIAGEN sciences (Germantown, MD, USA). Highly purified (affinity column purified and stated by the company to have undetectable LPS, soybean lectin LEC from Glycine max) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Trans-resveratrol (RES > 99% HPLC pure) was also purchased from Sigma-Aldrich (St. Louis, MO, USA). PBMCs of a normal healthy individual and PwD2 were purchased from STEMCELL technologies (Vancouver, BC, Canada) Technologies or from Precision for Medicine (Frederick, MD, USA).