Cytokine assay kits

Manufactured by Bio-Rad

Cytokine assay kits are laboratory equipment used to measure the levels of cytokines, which are signaling proteins produced by various cells in the body. These kits provide a quantitative analysis of cytokine concentrations in biological samples, such as cell culture supernatants, serum, or plasma. The core function of cytokine assay kits is to enable researchers and scientists to study the role of cytokines in biological processes and disease states.

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Lab products found in correlation

Amine coupling kit, by Bio-Rad (1 mentions) Multiscreen filter plate, by Merck Group (1 mentions) Biotinylated aal, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Bio‐Plex multiplex cytokine assay kit Bio-Plex Pro Human Cytokine 27-plex Assay kit Bio-Plex Pro mouse cytokine assay kit Bio-Plex Pro human cytokine assay kit Multiplex cytokine assay kit Bio-Plex Pro™ Cytokine Assay Kit Bio-Plex Pro Human Cytokine Group I 12-plex assay kit Luminex Bio-Plex cytokine assay kit Bio-Plex Human Cytokine Group Assay kit Bio-Plex mouse cytokine 23-plex assay kit

2 protocols using cytokine assay kits

Multiplexed Lectin-based Protein Detection

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Agarose-bound Aleuria Aurantia Lectin (AAL) beads were purchased from Vector Labs (Burlingame, CA). Multiscreen filter plates were from Millipore (Billerica, MA). Bio-Plex Pro^TM magnetic COOH beads, amine coupling kits, and cytokine assay kits were purchased from Bio-Rad Laboratories (Hercules, CA). Biotinylated AAL was purchased from Vector Labs (Burlingame, CA). Biotinylated detection antibody was prepared with Thermo Scientific (Rockford, IL) EZ-link Sulfo-NHS-Biotin (Catalog #21326).
Human recombinant PSA (Catalog #PO725), human PSA mouse monoclonal antibody (Catalog #MP077-BP001) for capture, and biotinylated mouse monoclonal antibody (Catalog #MP007-AP002S) for detection were purchased from Scripps Laboratories (San Diego, CA). Mouse myeloma cell line NS0-derived human recombinant TIMP1 (Catalog #970-TM-010), human TIMP1 mouse monoclonal IgG2B antibody (Catalog #MAB970, clone #63515) for capture, and biotinylated human TIMP1 goat polyclonal IgG antibody (Catalog # BAF970) for detection were purchased from R&D Systems (Minneapolis, MN). Chinese Hamster ovary cell line CHO-derived human recombinant tPA protein (Catalog #ab92637), human tPA mouse monoclonal antibody (Catalog # ab82249) for capture, and biotinylated human tPA rabbit polyclonal IgG antibody (Catalog #ab28208) for detection were purchased from Abcam (Cambridge, MA).

Li Q.K., Chen L., Ao M.H., Chiu J.H., Zhang Z., Zhang H, & Chan D.W. (2015). Serum Fucosylated Prostate-specific Antigen (PSA) Improves the Differentiation of Aggressive from Non-aggressive Prostate Cancers. Theranostics, 5(3), 267-276.

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Cytokine Profiling of Cell Supernatants

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Cellular debris was removed by centrifuging cell culture supernatants at 4°C for 15 min at 16,200 g. Then, the supernatants were aliquoted and immediately preserved at −80°C before analysis. The levels of chemokines and cytokines in the supernatants were determined using Bio‐Rad cytokine assay kits in line with the guidance provided by the manufacturer.

Zhou B., Wang L., Yang S., Liang Y., Zhang Y., Liu X., Pan X, & Li J. (2024). Pyrogallol protects against influenza A virus‐triggered lethal lung injury by activating the Nrf2–PPAR‐γ–HO‐1 signaling axis. MedComm, 5(4), e531.

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