IL-6 levels in ascites samples were determined by ELISA using the commercially available human Quantikine HS ELISA Kit (R&D Systems, MN). The assays were performed in duplicate according to the manufacturer's protocols. The detection threshold was 0.156 pg/ml. A quantity of 50 μl of undiluted ascites was added in each well.
Quantikine hs elisa kit
Manufactured by R&D Systems
Sourced in United States
The Quantikine HS ELISA kit is a high-sensitivity enzyme-linked immunosorbent assay (ELISA) designed to quantitatively measure specific analytes in cell culture supernates, serum, plasma, and other biological fluids.
Automatically generated - may contain errors
Lab products found in correlation
Multiskan fc, by Thermo Fisher Scientific (2 mentions)
Serum separator tube, by BD (1 mentions)
19160 sod determination kit, by Merck Group (1 mentions)
Advia 1650 system, by Bayer (1 mentions)
Mature bdnf rapid elisa kit, by Biosensis (1 mentions)
Limulus amebocyte lysate assay, by Lonza (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Tristar2 s lb 942, by Berthold Technologies (1 mentions)
Meso scale discovery kits, by R&D Systems (1 mentions)
Hslb00d, by R&D Systems (1 mentions)
Imark microplate absorbance reader, by Bio-Rad (1 mentions)
Sample diluent, by R&D Systems (1 mentions)
Infinite m1000, by Tecan (1 mentions)
21 protocols using quantikine hs elisa kit
1
Quantifying IL-6 in Ascites by ELISA
2
Hemostatic and Inflammatory Responses to HBOT
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using a 22-gauge needle, 10 mL of blood was collected from the antecubital vein of each subject before exercise (BE), after exercise (AE), and after HBOT (AH) into a serum separator tube, an ethylenediamine tetraacetate-containing tube, and a sodium citrate-containing tube (all tubes were purchased from Becton Dickinson, Franklin Lakes, NJ, USA). The collected blood samples were centrifuged for 15 min at 3000 rpm and were then stored at −80 °C until analysis. The analysis of plasma fibrinogen levels was performed using a chronometric assay (Fibriprest, Diagnostica Stago, Asnieres, France). Serum IL-6 and TNF-α levels were measured using a human IL-6 Duoset enzyme-linked immunosorbent assay (ELISA) kit (DY206; R&D Systems, Minneapolis, MN, USA) and a human TNF-alpha Quantikine HS ELISA kit (HSTA00E; R&D Systems, Minneapolis, MN, USA), respectively. A microplate reader (SunriseTM; TECAN Austria GMBH, Grödig/Salzburg, Austria) was used to measure the absorbance at 450 nm for quantification. The analyses of serum derivatives of reactive oxygen metabolites (d-ROMs) and the biological antioxidant potential (BAP) levels were measured using a commercial assay kit (Diacron SRL, Proma, Italy) as described by Hussein et al. [22 (link)]. Serum CK and LDH levels were measured at 680 nm absorbance using a standard microwell plate reader (Molecular Devices, Orleans, CA, USA).
3
Antioxidant and Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enzymatic activity of SOD was selected as a measure of antioxidant changes in plasma. The analysis was performed using a 19160 SOD Determination Kit (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) following the manufacturer’s instructions. The SOD activity was calculated as the inhibition rate of the reduction of a water-soluble formazan dye by superoxide anion.
The protein level of IL1B was determined as a representative pro-inflammatory cytokine in plasma using a Quantikine HS ELISA Kit (#HSLB00D; R&D Systems, Minneapolis, MN, USA).
The protein level of IL1B was determined as a representative pro-inflammatory cytokine in plasma using a Quantikine HS ELISA Kit (#HSLB00D; R&D Systems, Minneapolis, MN, USA).
4
Measurement of Inflammatory Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum high-sensitivity C-reactive protein (hs-CRP) was measured with an ADVIA 1650 system (Bayer, Tarrytown, NY, USA) using a commercially available, high-sensitivity CRP-Latex(II) X2 kit (Seiken Laboratories Ltd., Tokyo, Japan), which allows detection of CRP in the 0.001–31 mg/dL range, as described in a previous report [31 (link)]. Plasma concentrations of interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) were measured using the Quantikine® HS ELISA Kit (R&D systems, Inc., Minneapolis, MN, USA), according to the manufacturer's instructions. The resulting color reaction was measured using the iMark™ microplate absorbance reader (Bio-Rad Laboratories, Hercules, CA, USA). The wavelength correction was set to 490 and 560 nm.
5
Biomarkers of Neurological Resilience
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We measured antioxidant response (GST and HO-1), neuroplasticity (BDNF), and the neuroinflammation (TNF-α) blood parameter. Experienced nurses collected blood through venipuncture in 5 mL separator tubes for serum and 5 mL EDTA tubes for plasma (Nipro Corporation, Osaka, Japan). The obtained serum and plasma samples were aliquoted and stored at –80°C.
BDNF has three different forms such as prodomain BDNF, mature BDNF, and precursor BDNF (Hempstead, 2015 (link)). The mature BDNF has neurotrophic and neuroprotective functions. In this study, we measured the serum levels of mature BDNF using a mature BDNF rapid ELISA kit (Biosensis, Thebarton, Australia). The serum levels of TNF-α were measured with and a Quantikine HS ELISA kit (R&D systems, Minneapolis, MN, United States), respectively, following the manufacturer’s instructions. The enzyme activity of GST was determined with 1-chloro-2,4-dinitrobenzene as the substrate, as described previously (Kikuchi et al., 2015 (link)). The plasma level of HO-1 was determined using a StressXpress HO-1 ELISA kit (StressMarq Biosciences, Victoria, BC, Canada) following the manufacturer’s instructions.
BDNF has three different forms such as prodomain BDNF, mature BDNF, and precursor BDNF (Hempstead, 2015 (link)). The mature BDNF has neurotrophic and neuroprotective functions. In this study, we measured the serum levels of mature BDNF using a mature BDNF rapid ELISA kit (Biosensis, Thebarton, Australia). The serum levels of TNF-α were measured with and a Quantikine HS ELISA kit (R&D systems, Minneapolis, MN, United States), respectively, following the manufacturer’s instructions. The enzyme activity of GST was determined with 1-chloro-2,4-dinitrobenzene as the substrate, as described previously (Kikuchi et al., 2015 (link)). The plasma level of HO-1 was determined using a StressXpress HO-1 ELISA kit (StressMarq Biosciences, Victoria, BC, Canada) following the manufacturer’s instructions.
6
Quantification of Inflammatory Markers in HIV Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IL-6, IP10 and sCD14 levels in the plasma samples from HIV infected patients and healthy controls were determined via specific ELISAs. For IL-6 determination, the Quantikine HS ELISA kit (R&D Systems) was used following the manufacturer’s protocol. The kit is designed to measure human IL-6 in serum, plasma and urine and has a sensitivity of 0.016–0.110 pg/mL. IP10 and sCD14 levels were determined using the Quantikine ELISA human IP10 and CD14 Immunoassay (R & D Systems) following the manufacturer’s protocol. The sensitivity of IP10 detection using the kit is 0.41–4.46 pg/mL and the minimum detectable dose (MDD) of human CD14 is less than 125 pg/mL. Plasma LPS levels were determined using the endpoint chromogenic Limulus Amebocyte Lysate (LAL) assay (Lonza) using the manufacturer’s recommendations. The kit shows linear absorbance at 405–410 nm in the concentration range of 0.1–1.0EU/mL endotoxin.
7
Quantification of Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma concentrations of TNF-α (Quantikine HS ELISA kit; R&D Systems), CXCL12 (Quantikine Immunoassay; R&D Systems), and carboxy-terminal telopeptide of type I collagen (CTX; Immunodiagnostic Systems, The Boldons, UK), as well as SF concentrations of RANKL (Quantikine Immunoassay; R&D Systems), were determined in accordance with the manufacturers’ instructions. Briefly, samples were incubated for 2–3 h at room temperature on monoclonal antibody-precoated plates, washed three times, and incubated for the next 2 h with HRP-conjugated (CXCL12, RANKL) or alkaline phosphatase-conjugated (TNF-α, CTX) specific antibodies. After another three washes, the reactions were visualized with substrate solution (tetramethylbenzidine) (CXCL12, RANKL) or substrate and amplifier solutions (nicotinamide adenine dinucleotide phosphate and amplifier enzymes) (TNF-α, CTX) and then arrested with hydrochloric or sulfuric acid, respectively. Optical density was determined within 15 minutes on a microplate reader (Bio-Rad Laboratories, Hercules, CA, USA) set to an excitation wavelength of 450 nm (CXCL12, RANKL) or 490 nm (TNF-α, CTX).
8
Serum IL6 Quantification by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The serum level of IL6 was determined using a high-sensitivity Quantikine HS ELISA Kit (R&D Systems Inc., Minneapolis, USA), according to the protocol. All samples were measured in duplicates.
9
Quantification of IL-7 Levels in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IL-7 levels in plasma samples were measured using Quantikine HS ELISA kit following the manufacturer instruction (R&D Systems). This assay was performed on patient samples in the kinetics study. Briefly, plasma samples were diluted in 1 in 2 using the diluent provided. All samples were assayed in duplicate. Pre- and post-cART samples from the same patient were assayed in the same plate. The inter-assay and intra-assay CV for this assay were 16.3% (n=6) and 9.33% (n=6), respectively. The standard concentrations for this assay ranged from 0.25-16 pg/ml.
10
Quantitative Cytokine Profiling in Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A highly sensitive quantitative sandwich enzyme immunoassay technique, Quantikine® HS ELISA KIT (R& D systems, Minneapolis, USA), was used to measure circulating serum levels of IL-6 in accordance with the manufacture’s instruction starting from 200 μL of diluted sera or standard solutions. After adding Stop Solution to each well and the optical density was measured by using a Thermo Scientific Multiskan® FC microplate reader, at 490 and 690 nm.
IL-1 levels were measured by pre-coated ELISA using Boster Human IL-1 Picokine TM ELISA KIT according to the manufacturer’s instruction staring from 100 µL of standard solutions, samples (dil. 1:3) or control. The reaction was stopped by adding 100 µL of Stop Solution and O.D. absorbance read by using the microplate reader, at 450 nm. IL-1 was decantable in 18 out of 62 patients.
TNF-and IFN-levels were also measured in the serum of patients by using comedically available highly sensitive Picokine ELISA KITs, but the cytokine levels were under the limit of detection of these KITs.
IL-1 levels were measured by pre-coated ELISA using Boster Human IL-1 Picokine TM ELISA KIT according to the manufacturer’s instruction staring from 100 µL of standard solutions, samples (dil. 1:3) or control. The reaction was stopped by adding 100 µL of Stop Solution and O.D. absorbance read by using the microplate reader, at 450 nm. IL-1 was decantable in 18 out of 62 patients.
TNF-and IFN-levels were also measured in the serum of patients by using comedically available highly sensitive Picokine ELISA KITs, but the cytokine levels were under the limit of detection of these KITs.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!