Suffoil® (OAT Agrio Co., Ltd., Tokyo, Japan) was mixed with water at a ratio of 1:30, 1:300 (manufacturer's recommended ratio), 1:3000, or 1:30 000 and emulsified in a Vortex‐Genie 2 mixer (Scientific Industries, Inc., Bohemia, NY, USA). Water and paraffin oil (#164‐00476; Fujifilm Wako Pure Chemical Corp., Osaka, Japan), both harmless to spider mite eggs [13 , 14 ], were used as controls.
Paraffin oil
Manufactured by Fujifilm
Sourced in Japan
Paraffin oil is a clear, odorless, and colorless mineral oil commonly used in laboratory equipment. It serves as a lubricant and cooling agent in various lab instruments and machinery.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using paraffin oil
1
Suffoil® Emulsion Protocol
2
Synthesis and Purification of DGI Monomer
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Dodecylglyceryl itaconate (DGI) as an amphiphilic
monomer was synthesized from scratch as described elsewhere.6 (link) The crude product was purified by silica gel
column chromatography with a mixture of hexane and ethyl acetate as
the eluant (1:1 by volume). The obtained product was further recrystallized
twice from an acetone/hexane mixture (1:1 by weight). Acrylamide (AAm)
(Wako) as the monomer was recrystallized from chloroform and N,N′-methylenebisacrylamide (MBAA)
(Wako) as the crosslinker was recrystallized from ethanol. Irgacure
2959 (Wako) as the initiator and SDS (Wako), sodium stearate (SST)
(Wako), dodecyltriethylamine chloride (DTAC) (Wako), N-butylamine
(NBA) (Wako) as co-surfactants were used as received. MilliQ water
was used to prepare the precursor solutions and swell the hydrogels.
Paraffin oil (Wako) was used to preserve the hydrogels at the prepared
state in order to prevent any swelling or dehydration.
monomer was synthesized from scratch as described elsewhere.6 (link) The crude product was purified by silica gel
column chromatography with a mixture of hexane and ethyl acetate as
the eluant (1:1 by volume). The obtained product was further recrystallized
twice from an acetone/hexane mixture (1:1 by weight). Acrylamide (AAm)
(Wako) as the monomer was recrystallized from chloroform and N,N′-methylenebisacrylamide (MBAA)
(Wako) as the crosslinker was recrystallized from ethanol. Irgacure
2959 (Wako) as the initiator and SDS (Wako), sodium stearate (SST)
(Wako), dodecyltriethylamine chloride (DTAC) (Wako), N-butylamine
(NBA) (Wako) as co-surfactants were used as received. MilliQ water
was used to prepare the precursor solutions and swell the hydrogels.
Paraffin oil (Wako) was used to preserve the hydrogels at the prepared
state in order to prevent any swelling or dehydration.
3
Microinjection of C. elegans Gonads
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The wild-type C. elegans strain Bristol N2 was obtained from the Caenorhabditis Genetics Center (Minneapolis, MN). C. elegans was maintained using standard techniques (58 (link)). Young adult hermaphrodites were injected by using the standard procedures (59 (link)), with some modifications. In brief, glass capillaries (Narishige, ND-1) were pulled using a pipette puller (Olympus, PC-100). Needles were filled with the PG-ND dispersion. They were mounted on a manipulator (Narishige, MN-4) and pressurized through an injection system (Narishige, IM-31). Worms were immobilized on agarose injection pads that were covered with paraffin oil (Wako, Japan). The microscope used for the injection was equipped with differential interference contrast (Olympus, IX73). The PG-ND dispersion was injected into the distal arm of the gonad (fig. S6). The injected worms recovered on bacteria-seeded nematode growth medium plates for more than a day.
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