Immature DCs exposed to combinations of immethridine (1 μM) and/or LPS (100 ng/ml) for 12 h were stained with a FITC-labeled anti-CD11c and PE-labeled anti-CD40 or anti-CD86 (BD Biosciences, San Jose, CA). FACS analysis was performed on a flow cytometer (Becton Dickinson, USA) using CellQuest software (BD Biosciences). Results are expressed as mean fluorescent density (MFI).
Fitc labeled anti cd11c
Manufactured by BD
Sourced in United States
FITC-labeled anti-CD11c is a monoclonal antibody that binds to the CD11c antigen, which is expressed on the surface of dendritic cells and some subsets of monocytes and macrophages. The antibody is conjugated with the fluorescent dye fluorescein isothiocyanate (FITC), allowing for the detection and analysis of CD11c-positive cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Cellquest software, by BD (1 mentions)
Apc labeled anti cd3, by BD (1 mentions)
Pe labeled anti foxp3, by Thermo Fisher Scientific (1 mentions)
Fitc labeled anti cd8, by BD (1 mentions)
Pe labeled anti cd4, by BD (1 mentions)
Fitc labeled anti igg1, by BD (1 mentions)
Fitc labeled anti cd4, by Thermo Fisher Scientific (1 mentions)
Pe labeled anti cd11b, by BD (1 mentions)
Cellquest pro software, by BD (1 mentions)
Apc labeled anti cd86, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Lsr 2 flow cytometer, by BD (1 mentions)
Mhcii, by BD (1 mentions)
Pe labeled anti foxp3, by BD (1 mentions)
Fitc labeled anti cd4, by BD (1 mentions)
4 protocols using fitc labeled anti cd11c
1
Dendritic Cell Activation Assay
2
Immune Cell Profiling in MRL.Fas(lpr) Mice
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Spleens were isolated from MRL.Faslpr mice at 25 weeks of age, and immune cell composition was analyzed by flow cytometry. Spleen cells were stained with the following monoclonal antibodies in 0.5% BSA/PBS at 4°C for 15 min: FITC-labeled anti-B220, APC-labeled anti-CD3, PE-labeled anti-CD4, FITC-labeled anti-CD8, FITC-labeled anti-CD11c, PE-labeled anti-CD11b, APC-labeled anti-CD138, FITC-labeled anti-IgG1 (BD Biosciences, San Jose, CA, USA), FITC-labeled anti-CD4, and PE-labeled anti-Foxp3 (eBioscience). Data were collected using FACSCalibur and analyzed with CellQuest Pro software (BD Biosciences).
3
Dendritic Cell Surface Marker Analysis
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DCs cultured with or without aspirin were prepared for surface marker expression analysis by FACS staining. Cells were incubated on ice for 15–30 min with specific monoclonal antibodies (mAbs): FITC-labeled anti-CD11c, PerCP/Cy5.5-labeled anti-MHC class II, PE-labeled anti-CD80, and APC-labeled anti-CD86 (BD Biosciences, USA). After staining, the labeled cells were analyzed with a BD FACSCanto II flow cytometer (BD Biosciences).
4
Multicolor Flow Cytometry Analysis
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After cell density was adjusted to 1×106/mL, 100 μL single cell suspensions were added into flow tube and incubated with FITC-labeled anti-CD4, APC-labeled anti-CD25 and anti-IL-17, PE-labeled anti-Foxp3, FITC-labeled anti-CD11c, MHC-II, CD80, CD86 and Pir-B mAb (all from BD Pharmingen) for 30 min at 37°C. To detect IL-17+ or Foxp3+ cells, these cells were fixed and permeabilized. The appropriate fluorescein-conjugated IgG were used as isotype control. Cells were analyzed using a BD LSRII flow cytometer (Becton Dickinson, San Diego, CA) on at least 5,000-10,000 events.
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