Eclipse ci series

After tying one side of the bronchus and cutting half of the lung to prevent formalin entry, 10% (v/v) neutral-buffered formalin was injected through the airway for tissue fixation. After 24 h, the tissue was dehydrated, embedded in paraffin, and cut into 4 μm sections. The sections were then stained with hematoxylin and eosin and examined under a light microscope (Eclipse Ci series, Nikon, Tokyo, Japan).

DU-145 tumor sections and de-identified tissues from metastatic PCa patients obtained from the KCI Biobanking Core were stained for TRA using previously established protocols 33 (link). Mouse livers and kidneys were collected and fixed immediately in 4 % formaldehyde for 24 h, transferred and stored in 70% ethanol before paraffin-embedding. Tissue sections (5 μm thick) were mounted on glass slides with sections stained with hematoxylin and eosin (H&E). H&E-stained slides from the liver and kidney samples were examined with an optical microscope (Nikon Eclipse Ci Series, Tokyo, Japan) for histologic signs of radiation-induced injury. DU-145 tumor tissues stained for Ki-67 (clone 8D5; Cell Signaling Technologies, Cat # 9449T) were scanned and images were obtained using a Leica APERIO CS2 scanner. Proliferative cells were analyzed using Leica Aperio ImageScope v. 12.3.3 and expressed as the mean percentage of positively stained cells obtained from at least three different regions of the tumor section.