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Tguide s32 magnetic blood genomic dna kit

Manufactured by Tiangen Biotech
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Sourced in China

The TGuide S32 Magnetic Blood Genomic DNA Kit is a lab equipment product designed for the rapid and efficient extraction of genomic DNA from whole blood samples. It utilizes magnetic bead-based technology to capture and purify DNA, providing a reliable and consistent method for genomic DNA isolation.

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Lab products found in correlation

Agilent 2100 bioanalyzer, by Agilent Technologies (7 mentions) Qubit dsdna hs assay kit, by Thermo Fisher Scientific (4 mentions) Tianamp genomic dna kit, by Tiangen Biotech (3 mentions) Qubit dsdna hs high sensitivity assay kit, by Thermo Fisher Scientific (3 mentions) Magpure ffpe dna kit b, by Magen Biotechnology Co (3 mentions) Magmax cell free dna cfdna isolation kit, by Thermo Fisher Scientific (2 mentions) Accugreen high sensitivity dsdna quantitation kit, by Biotium (2 mentions) Le220, by Covaris (2 mentions) Novaseq 6000, by Illumina (1 mentions) Kapa htp library preparation kit, by Illumina (1 mentions) Magmax cell free dna isolation, by Thermo Fisher Scientific (1 mentions) Kapa hyper prep kit, by Roche (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Agencourt ampure xp bead, by Beckman Coulter (1 mentions) Magmax cell free dna isolation kit, by Thermo Fisher Scientific (1 mentions) Generead dna ffpe kit, by Qiagen (1 mentions) Agilent hs dna kit, by Agilent Technologies (1 mentions) Kapa hyper preparation kit, by Roche (1 mentions)

Close spelling variants of this product

Genomic DNA Kit (78 citations) Blood DNA kit (38 citations)

8 protocols using tguide s32 magnetic blood genomic dna kit

1

Genomic DNA Extraction from FFPE and Blood

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DTumor tissue DNA was extracted from formalin-fixed, paraffin-embedded samples using a TIANamp Genomic DNA Kit (TIANGEN, Beijing, China). Genomic DNA was extracted from the peripheral blood cells using the TGuide S32 Magnetic Blood Genomic DNA Kit (TIANGEN, China). The DNA concentration was measured using Qubit dsDNA HS Assay Kit (Themo Fisher Scientific, Waltham, MA, USA), and its quality was evaluated using Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA).
Zhang Q., Feng X., Hu W., Li C., Sun D., Peng Z., Wang S., Li H, & Zhou M. (2023). Chronic obstructive pulmonary disease alters the genetic landscape and tumor immune microenvironment in lung cancer patients. Frontiers in Oncology, 13, 1169874.
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2

Efficient DNA Extraction Protocols

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The genomic DNA of PBL was extracted using the TGuide S32 Magnetic Blood Genomic DNA Kit (Tiangen). cfDNA in plasma was extracted using the MagMAX Cell‐Free DNA (cfDNA) Isolation kit (Thermo Fisher Scientific). The genomic DNA of tumour tissue was extracted from formalin‐fixed paraffin‐embedded (FFPE) samples using the MagPure FFPE DNA Kit B (Magen). The concentration of extracted DNA was measured using the Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher Scientific), and the quality of extracted DNA was assessed using the Agilent 2100 BioAnalyzer (Agilent).
Xu E., Su K., Zhou Y., Gong L., Xuan Y., Liao M., Cao J., Li Y., Lu Y., Zhao Y, & Chen F. (2021). Comprehensive landscape and interference of clonal haematopoiesis mutations for liquid biopsy: A Chinese pan‐cancer cohort. Journal of Cellular and Molecular Medicine, 25(21), 10279-10290.
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3

Comprehensive Genomic Profiling of FFPE Tumors

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DNA was isolated from formalin-fixed paraffin-embedded (FFPE) tumor specimens with the TIANamp genomic DNA kit (TIANGEN, China) according to the manufacturers’ instructions. Genome DNA is extracted by TGuide S32 magnetic blood genomic DNA kit (TIANGEN, China) from peripheral blood lymphocyte (PBL), and circulating cell-free DNA (cfDNA) is extracted by MagMAX cell-free DNA isolation (ThermoFisher, USA) from the plasma sample. Fragmented DNA libraries were constructed with a KAPA HTP library preparation kit (Illumina Platform) (KAPA Biosystems, Massachusetts, USA) according to the manufacturer’s instructions. All libraries were quantified using AccuGreen high sensitivity dsDNA quantitation kit (Biotium, USA), with library size assessed on agilent bioanalyzer 2100 (Agilent, USA). DNA libraries from baseline tissue samples were captured with Panel 1, which was a designed panel spanning 769 cancer-related genes (Genecast, Wuxi, China), while DNA libraries from plasma samples were captured with panel 2, which covered exon regions of 95 genes (Genecast, Wuxi, China) related to drug resistance. The captured library was sequenced on Illumina Novaseq 6000 with paired end 150 bp mode.
Mao S., Yang S., Liu X., Li X., Wang Q., Zhang Y., Chen J., Wang Y., Gao G., Wu F., Jiang T., Zhang J., Yang Y., Lin X., Zhu X., Zhou C, & Ren S. (2023). Molecular correlation of response to pyrotinib in advanced NSCLC with HER2 mutation: biomarker analysis from two phase II trials. Experimental Hematology & Oncology, 12, 53.
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4

DNA Extraction and Sequencing Library Prep

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DNA was extracted from tumor tissues using GeneRead DNA FFPE Kit (Qiagen 180134, Hilden, Germany) and from peripheral blood lymphocyte with TGuide S32 Magnetic Blood Genomic DNA Kit (TIANGEN DP601-T5C China) according to the manufacturer’s recommended protocol. Cell free DNA (cfDNA) was extracted using MagMAX Cell-Free DNA Isolation kit (ThermoFisher, A29319 USA). DNA samples were quantified with the Qubit dsDNA HS Assay kit (Life Technologies, Q32854 USA) following the manufacturer’s instructions. Genomic DNA was sheared into 150-200 base pairs (bp) fragments through Covaris LE220 using the recommended settings for NGS library preparation. KAPA Hyper Prep Kit (KAPA Biosystems, Massachusetts, KK8504 USA) was used for fragmented DNAs construction according to the manufacturer’s instruction. All fragmented gDNA or cfDNA (10 to 50 ng) was end-repaired, sequencing adaptor-ligated, and PCR amplified before purification with 0.8X Agencourt AMPure XP beads (Beckman Coulter, Brea, CA, A63882 USA). The concentration and quality of the library was determined using the Qubit 3.0 system and Bioanalyzer 2100 (Agilent, Agilent HS DNA Kit, 5067-4626).
Jiang T., Chen J., Xu X., Cheng Y., Chen G., Pan Y., Fang Y., Wang Q., Huang Y., Yao W., Wang R., Li X., Zhang W., Zhang Y., Hu S., Guo R., Shi J., Wang Z., Cao P., Wang D., Fang J., Luo H., Geng Y., Xing C., Lv D., Zhang Y., Yu J., Cang S., Zhang Y., Zhang J., Yang Z., Shi W., Zou J., Zhou C, & Ren S. (2022). On-treatment blood TMB as predictors for camrelizumab plus chemotherapy in advanced lung squamous cell carcinoma: biomarker analysis of a phase III trial. Molecular Cancer, 21, 4.
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5

DNA Extraction from FFPE and PBL Samples

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Genomic DNA of tumor was extracted from formalin-fixed paraffin-embedded (FFPE) samples using MagPure FFPE DNA Kit B (Magen, China, ID: D6323-02). Genomic DNA of peripheral blood lymphocyte (PBL) was extracted using TGuide S32 Magnetic Blood Genomic DNA Kit (Tiangen, China, ID: DP601). The concentration of DNA was measured by Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher, USA, ID: Q32851), while the quality of DNA was assessed by Agilent 2100 BioAnalyzer (Agilent, USA). All samples for DNA extraction were obtained before the treatments started.
Zhan Q., Wen C., Zhao Y., Fang L., Jin Y., Zhang Z., Zou S., Li F., Yang Y., Wu L., Jin J., Lu X., Xie J., Cheng D., Xu Z., Zhang J., Wang J., Deng X., Chen H., Peng C., Li H., Zhang H., Fang H., Wang C, & Shen B. (2021). Identification of copy number variation-driven molecular subtypes informative for prognosis and treatment in pancreatic adenocarcinoma of a Chinese cohort. EBioMedicine, 74, 103716.
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6

Imprint Cytology-Guided Tumor Extraction

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Imprint cytology was performed to evaluate tumor purity before DNA extraction. Briefly, freshly cut surfaces of tissue specimens were gently pressed to glass slides. Then the slides was stained with hematoxylin and eosin (HE) after fixing with 95% of ethyl alcohol for 5–6 s. If the percentage of tumor cells was higher than 15%, the specimen was considered qualified for subsequent extraction and sequencing. Genomic DNA was extracted from fresh tumor tissue using the TIANamp Genomic DNA Kit (TIANGEN, China). Genomic DNA from peripheral blood lymphocytes (PBL) was extracted using a TGuide S32 Magnetic Blood Genomic DNA Kit (TIANGEN, China). The concentration of DNA was measured using a Qubit dsDNA HS Assay Kit (Thermo Fisher, USA), whereas the DNA quality was assessed using an Agilent 2100 BioAnalyzer (Agilent, USA). All extractions and assays were conducted according to the manufacturers’ instructions supplied in the respective kits used in this study.
Jiang C., Lu Y., Liu H., Cai G., Peng Z., Feng W, & Lin L. (2023). Clinical characterization and genomic landscape of gynecological cancers among patients attending a Chinese hospital. Frontiers in Oncology, 13, 1143876.
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7

Efficient DNA Extraction from PBLs and cfDNA

Cited 1 time
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The method for DNA extraction was as described by Xu et al. [10 (link)]. Genomic DNA was extracted from the PBLs using the TGuide S32 Magnetic Blood Genomic DNA Kit (Tiangen, China). Plasma cfDNA was isolated with the MagMAX Cell-Free DNA (cfDNA) Isolation kit (Thermo Fisher Scientific, USA). The concentration of extracted DNAs was measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, USA), and the DNA quality was assessed by the Agilent 2100 BioAnalyzer (Agilent, USA).
Huang Q., Zhou Y., Wang B., Zhao Y., Zhang F, & Ding B. (2022). Mutational landscape of pan-cancer patients with PIK3CA alterations in Chinese population. BMC Medical Genomics, 15, 146.
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8

FFPE DNA Extraction and Library Prep

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Genomic DNA was extracted from FFPE samples using the MagPure FFPE DNA Kit B (Magen, China). Peripheral blood lymphocyte (PBL) DNA was extracted by the TGuide S32 Magnetic Blood Genomic DNA Kit (Tiangen, China). The DNA concentration was measured by the Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher, USA). The quality of DNA was assessed by an Agilent 2100 BioAnalyzer (Agilent, USA). DNA (50-300 ng) from FFPE samples was sheared with Covaris LE220 according to the recommended settings for ~200 bp fragments, and the fragmented DNA was input for library construction by the KAPA Hyper Preparation Kit (Kapa Biosystems, USA) according to the manufacturer's instructions. All libraries were quantified by the AccuGreen High Sensitivity dsDNA Quantitation Kit (Biotium, USA), and the size of the libraries was determined on the Agilent Bioanalyzer 2100 (Agilent, USA).
Ju S., Cui Z., Hong Y., Wang X., Mu W., Xie Z., Zeng X., Su L., Zhang Q., Song X., You S., Chen R., Chen W., Xuchun, & Zhao J. (2021). Detection of Multiple Types of Cancer Driver Mutations Using Targeted RNA Sequencing in NSCLC.
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