The following reagents were purchased from the indicated manufacturers: rabbit polyclonal anti-human DR4 antibody, anti-human TRPV1 antibody, and anti-human HDAC1 antibody from Abcam (Cambridge, UK); mouse polyclonal anti-β-actin antibody and valproate from Sigma (St. Louis, MO); rabbit polyclonal anti-Histone H3 antibody, rabbit polyclonal anti-acetyl-histone H3 antibody and anti-acetyl-histone H4 antibody from Merck Millipore (Billerica, MA); rabbit polyclonal anti-Sp1 antibody, anti-Akt antibody, anti-phosphorylated Akt antibody, anti-PI3K antibody, anti-phosphorylated PI3K antibody, anti-STAT3 antibody, anti-phosphorylated STAT3 antibody, anti-p38 antibody, anti-phosphorylated p38 antibody, anti-p65 antibody, anti-phosphorylated p65 antibody, anti-ERK antibody, anti-phosphorylated ERK antibody, anti-JNK antibody, anti-phosphorylated JNK antibody, horseradish peroxidase (HRP)-anti-rabbit IgG and anti mouse IgG from Cell Signaling Technology (Beverly, MA); rh IGF-1 from R&D Systems (Minneapolis, MN); panobinostat from Cayman Chemical Company (Ann Arbor, MI); and 5-azacytidine and Akt inhibitor VIII from Calbiochem (Darmstadt, Germany). The human monoclonal anti-DR4 agonistic IgG antibody R1-B12 was kindly provided by Kyowa Hakko Kirin Co. Ltd. (Tokyo, Japan).
Anti phosphorylated stat3 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-phosphorylated STAT3 antibody is a laboratory reagent designed to detect and bind to the phosphorylated form of the STAT3 protein. STAT3 is a transcription factor that plays a central role in cellular signaling pathways. The antibody can be used to identify and quantify the levels of phosphorylated STAT3 in biological samples through various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti stat3 antibody, by Cell Signaling Technology (3 mentions)
Anti rabbit igg horseradish peroxidase hrp, by Cell Signaling Technology (1 mentions)
Anti jnk antibody, by Cell Signaling Technology (1 mentions)
Anti erk antibody, by Cell Signaling Technology (1 mentions)
Anti p38 antibody, by Cell Signaling Technology (1 mentions)
Anti p65 antibody, by Cell Signaling Technology (1 mentions)
Anti acetyl histone h4 antibody, by Merck Group (1 mentions)
Panobinostat, by Cayman Chemical (1 mentions)
Anti phosphorylated erk antibody, by Cell Signaling Technology (1 mentions)
Anti phosphorylated p38 antibody, by Cell Signaling Technology (1 mentions)
Anti phosphorylated akt antibody, by Cell Signaling Technology (1 mentions)
Anti pi3k antibody, by Cell Signaling Technology (1 mentions)
Rhigf1, by R&D Systems (1 mentions)
Anti cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by Cell Signaling Technology (1 mentions)
Anti eif2α antibody, by Cell Signaling Technology (1 mentions)
Anti perk antibody, by Santa Cruz Biotechnology (1 mentions)
4 protocols using anti phosphorylated stat3 antibody
1
Signaling Pathway Regulation in Cell Lines
2
Western Blot Analysis of Protein Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse tissue or cultured NRCMs were homogenized in lysis buffer (cell signaling technology, #9803) with 1 mM phenylmethylsulfonyl fluoride (PMSF), and lysates were separated on 5–20% polyacrylamide gels and transferred to polyvinylidene difluoride membranes. After blocking with 5% skim milk, the membranes were probed with one of the following primary antibodies overnight at 4 °C: anti PERK antibody (1:200, Santa cruz), anti p-PERK (Thr980) antibody (1:100, cell signaling technology), anti eIF2α antibody (1:1000, cell signaling technology), anti p-eIF2α (Ser51) antibody (1:1000, cell signaling technology), anti PRL (N-terminal) antibody (1:1000, Acris Antibodies GmbH), anti phosphorylated STAT3 antibody (1:2000, cell signaling technology), anti STAT3 antibody (1:1000, cell signaling technology), anti cleaved caspase3 antibody (1:1000, cell signaling technology), anti GAPDH antibody (1:1000, cell signaling technology), and anti transferrin antibody (1:1000, Santa cruz).
This was followed by a 1-h incubation with secondary antibodies conjugated with horseradish peroxidase (HRP) at room temperature. Bound antibodies were detected by chemiluminescence with the ECL detection system. Relative protein levels were quantified using the Image J program (NIH, Bethesda, MD). Some of images (Figs.1 A,F, 3 D) did not include full length membranes, with membrane edges visible.
This was followed by a 1-h incubation with secondary antibodies conjugated with horseradish peroxidase (HRP) at room temperature. Bound antibodies were detected by chemiluminescence with the ECL detection system. Relative protein levels were quantified using the Image J program (NIH, Bethesda, MD). Some of images (Figs.
3
Antibody Validation for Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-stat3 antibody and anti-phosphorylated stat3 antibody were purchased from Cell Signaling Technology. Anti-G-CSFR antibody was purchased from Abcam.
4
Immunohistochemical Analysis of Liver Regeneration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissues fixed in formaldehyde were embedded in paraffin and sectioned into 3μm thickness. After deparaffinization, antigen retrieval and the removal of endogenous peroxidase, the sections were incubated overnight at 4 °C with a mouse monoclonal anti-PCNA antibody (1:200 dilution; Dako, Glostrup, Denmark), anti-phospho histone H3 antibody (1:500 dilution; Proteintech Group, Chicago, IL, USA), anti-Reg3α antibody (1:50 dilution; R&D Systems), anti-Reg3β antibody (1:50 dilution; R&D Systems) or a rabbit monoclonal anti-phosphorylated STAT3 antibody (1:200 dilution; Cell Signaling Technology, Danvers, MA, USA), followed by an incubation for 30 min at room temperature with TaKaRa POD Conjugate For Tissue (Takara Bio, Shiga, Japan) for visualization. The sections were immersed in diaminobenzidine solution and counterstained with hematoxylin.
To evaluate hepatocyte proliferation on days 1 and 3, the average percentage of PCNA-positive cells to total hepatocytes in 10 random high-power fields was established as the PCNA labeling index.
To evaluate hepatocyte proliferation on days 1 and 3, the average percentage of PCNA-positive cells to total hepatocytes in 10 random high-power fields was established as the PCNA labeling index.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!