SPE procedures were carried out using a Visi-1 Single SPE Tube Processor purchased from Sigma-Aldrich (Germany). pH measurements were done using Jenway pH meter 3310 (UK). Ultrapure water was prepared using the Stakpure Pure water system OMNIATYPE1, Stakpure GmbH (Germany). Fourier transform infrared (FTIR) spectra were recorded using Thermo Scientific™ Nicolet™ iS50 FTIR spectrometer (Germany). The surface area, pore volume, and pore size of the polymers were calculated using Brunauer–Emmett–Teller (BET) and Barret-Joyner-Halenda (BJH) methods via NOVAtouch® LX4 Quantachrome (USA). Surface morphology was examined using the JEOL JSM-6360LA microscope for field-emission scanning electron microscopy (SEM) (Japan). All the analytical measurements were carried out on ACQUITY UPLC H-Class system that is composed of Xevo™ TQD triple-quadrupole tandem mass analyzer with an electrospray ionization (ESI) interface, Mass Lynx 4.1 software, and target Lynx quantification program, all provided by Waters Corp. (USA). Acquity UPLC BEH C18 column (1.7 µm, 100 mm × 2.1 mm) was used to separate the target analytes (Waters, Wexford, Ireland).
Target lynx quantification program
Manufactured by Waters Corporation
Sourced in United States
The Target Lynx quantification program is a software application developed by Waters Corporation. It is designed to facilitate the quantification of target analytes in complex sample matrices using liquid chromatography-mass spectrometry (LC-MS) data. The program provides a comprehensive set of tools for data processing, peak integration, and quantification, enabling users to analyze and interpret their experimental results.
Automatically generated - may contain errors
Lab products found in correlation
Masslynx 4, by Waters Corporation (4 mentions)
Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)
Acquity uplc beh c18 column, by Waters Corporation (1 mentions)
Jsm 6360 la microscope, by JEOL (1 mentions)
Xevo tq s tandem quadrupole mass spectrometry, by Waters Corporation (1 mentions)
Xevo tq s tandem quadrupole mass spectrometer, by Waters Corporation (1 mentions)
Quattro premier xe triple quadrupole ms, by Waters Corporation (1 mentions)
4 protocols using target lynx quantification program
1
Comprehensive Analytical Workflow for Polymers
2
Tandem-Quadrupole Mass Spectrometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Xevo TQ-S tandem-quadrupole mass spectrometry (Waters, Manchester, United Kingdom) equipped with a Z-spray electrospray interface was used. Positive electrospray ionization was performed in the multiple reaction monitoring (MRM) mode. The capillary voltage was set to 1.0 kV, the source block temperature was set to 120°C, the desolvation gas (nitrogen) was heated to 650°C and delivered at a flow rate of 1000 L/h, and the cone gas (nitrogen) was set to 150 L/h. Dwell times were automatically adjusted with 15–20 data points per peak. MRM transitions, cone voltage, and collision energy for the analytes and IS used in the presented method are shown in Table 1 . System operation and data acquisition were controlled using Mass Lynx 4.1 software (Waters). All data were processed with the Target Lynx quantification program (Waters).
3
Quantification of Citalopram Enantiomers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Xevo TQ-S tandem-quadrupole mass spectrometer (Waters, Manchester, UK) equipped with a Z-spray electrospray interface was used. Positive electrospray ionization (ESI) was performed in the multiple reaction monitoring (MRM) mode. The capillary voltage was set to 3.0 kV, the source block temperature was 120 ºC, the desolvation gas (nitrogen) was heated to 500 ºC and delivered at a flow rate of 1000 L/h, and the cone gas (nitrogen) was set to 150 L/h. Dwell times were automatically adjusted with 15-20 data points per peak. The m/z 325.1 > 262.0 (cone voltage: 30 V, collision energy: 18 eV) and m/z 325.1 > 109.0 (cone voltage: 30 V, collision energy: 25 eV) transitions were monitored for R/S-citalopram. The m/z 331.1 > 262.0 (cone voltage: 30 V, collision energy: 18 eV) was monitored for S-citalopram-d 6 and m/z 329.4 > 266.0 (cone voltage: 30 V, collision energy: 18 eV) for R/S-citalopram-d 4 . System operation and data acquisition were controlled using Mass Lynx 4.1 software (Waters, Manchester, UK). All data were processed with the Target Lynx quantification program (Waters, Manchester, UK).
4
UHPLC-MS/MS Analysis of PALME Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UHPLC-MS/MS analysis of PALME extracts obtained from whole blood samples was performed using an integrated system from Waters (Milford, MA, USA) with an Acquity TM Ultra Performance LC. Chromatographic separation was performed at 65 • C on an Acquity UPLC ® HSS T3 column with a length of 100 mm, an inner diameter of 2.1 mm, a particle size of 1.8 m, and a pore size of 100 Å. Data acquisition was performed using MassLynx 4.1 software (Waters Corp., Milford, MA, USA), and data were processed with the Target-Lynx quantification program (Waters Corp.). Mobile phase A and B consisted of 10 mM ammonium formate (pH 3.1) and methanol, respectively. The flow rate was 0.5 mL min -1 , and the analysis was performed with the following gradient: 2.5% B at 0.00 min, 35% B at 6.75 min, 65% B at 8.00 min, 100% B at 8.01 min, 100% B at 9.00 min, and 2.5% B at 9.01 min. Total run time was 11 min, and the injection volume was 3.0 L. MS detection was performed on a Waters Quattro Premier XE triplequadrupole MS, (Waters Corp., Milford, MA, USA). Ionization was performed with electrospray ionization in the positive mode with a voltage of 1 kV. Analysis was performed using selected reaction monitoring. The SRM transitions and collision energies are presented in Table 2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!