Sybr green qpcr supermixes

Manufactured by Bio-Rad

Sourced in United States

SYBR Green qPCR Supermixes are ready-to-use solutions for quantitative real-time PCR (qPCR) experiments. They contain SYBR Green, a fluorescent dye that binds to double-stranded DNA, and all the necessary components for qPCR amplification and detection.

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Lab products found in correlation

Primescript rt reagent kit, by Takara Bio (3 mentions) Cfx 192 connect real time pcr system, by Bio-Rad (2 mentions) Cell total rna isolation kit, by Foregene (2 mentions) Iq5 real time pcr detection system, by Bio-Rad (1 mentions) Mirna real time pcr quantitation kit, by Thermo Fisher Scientific (1 mentions) Moloney murine leukemia virus reverse transcription kit, by Promega (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

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SYBR Green Supermix (1501 citations) SYBR Green (1276 citations) SYBR Green qPCR SuperMix (26 citations) SYBR Green Supermixes (14 citations)

4 protocols using sybr green qpcr supermixes

Quantitative Analysis of Gene Expression in Gastric Cancer

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A total of 14 pairs of GC samples (Additional file 1: Table S1) were collected and the relative expression of genes in the RS model was detected. The study was approved by the Ethics Committee of Renmin Hospital of Wuhan University (No. NCT03972956V1.1), and we have obtained informed consents from the patients for using GC samples (No. SAMPGICU2019-2). Total RNA was extracted using Tissue Total RNA Isolation Kit (Foregene, Wuhan, China) as per the manufacturer’s protocol and RNA (2 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent Kit (TaKaRa, Osaka, Japan). Quantitative PCR (qPCR) was performed using the SYBR Green qPCR Supermixes (Bio-Rad) on the CFX 192 Connect Real-Time PCR system (Bio-Rad, USA). The qPCR analysis was performed in triplicates with the designed primers (Table 1). The relative expression was normalized to GAPDH using the 2-ΔΔCT method.Table 1

List of primers

Gene	Primer sequence (5′–3′)
SLIT2	Forward: GCACCATTGAAAGAGGAGCA
SLIT2	Reverse: GCTTTCCTTGGGATTGCCTG
SFRP2	Forward: ACCGAGGAAGCTCCAAAGGTA
SFRP2	Reverse: GAGCCACAGCACCGATTTCT
SCRG1	Forward: CATTTCTGGGATGGGAAGGGA
SCRG1	Reverse: GTGGGAAATCAGGAATGGTGTT
MFAP5	Forward: CAGCGTAAGAGGAGAGAGACAC
MFAP5	Reverse: CAGCAAGAAACAGCAGCACCT
EFEMP1	Forward: TGAAATGCAGACTGGCCGAA
EFEMP1	Reverse: TCTACAGTTGTGCGTCCCTG
COL8A1	Forward: AAGGAGATGCCCCACTTGC
	Reverse: GGACCTTGTTCCCCTCGTAA
ABCA8	Forward: AAGAACGCAAAACAGACCGC
ABCA8	Reverse: TTTGGCATCAGGGATGTGCT

Xiang R., Song W., Ren J., Wu J., Fu J, & Fu T. (2021). Identification of stem cell-related subtypes and risk scoring for gastric cancer based on stem genomic profiling. Stem Cell Research & Therapy, 12, 563.

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Quantitative RT-PCR Analysis of Hub Genes

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Total RNA was extracted from cell lines using Cell Total RNA Isolation kit (Foregene, Chengdu, China) following the manufacturer’s instruction. RNA (1 μg) was reverse‐transcribed to complementary DNA (cDNA) according to the protocol of the PrimeScript RT reagent kit (TaKaRa, Osaka, Japan). qRT-PCR was carried out on the iQ5 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) using the SYBR Green qPCR Supermixes (Bio-Rad). Primer sequences were listed in Table 2. Cycle threshold (Ct) of each gene was standardized to GAPDH using the 2^−ΔΔCT method. Each experiment was conducted in triplicate.

Table 2

Primer sequences for five hub genes and β-actin

Gene	Primer sequence
PDCD6	Forward: ATGGCCGCCTACTCTTACC
PDCD6	Reverse: TCCTGTCTTTATCGACCCTCTG
TCOF1	Forward: AGTCTCCAGCAAGAAAGGCG
TCOF1	Reverse: TCCTCTTCTGGCTTCTTGGC
TRIM28	Forward: TGTTTCCACCTGGACTGTCA
TRIM28	Reverse: CCAGCAGTACACGCTCACAT
EZH2	Forward: TGCAGTTGCTTCAGTACCCATAAT
EZH2	Reverse: ATCCCCGTGTACTTTCCCATCATAAT
FAM83D	Forward: AGAGCGGCAATTCCACTTCG
FAM83D	Reverse: TGCCAGAATGAAGGCCAAGG
β-actin	Forward: CATGTACGTTGCTATCCAGGC
β-actin	Reverse: CTCCTTAATGTCACGCACGAT

Zhu G., Xia H., Tang Q, & Bi F. (2021). An epithelial-mesenchymal transition-related 5-gene signature predicting the prognosis of hepatocellular carcinoma patients. Cancer Cell International, 21, 166.

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Quantification of miRNA Expression

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Total RNA was isolated using TRIzol reagent (Invitrogen) and reverse-transcribed using Moloney murine leukemia virus reverse transcription kit (Promega, Madison, WI, USA). qRT-PCR was conducted by using SYBR^® Green qPCR supermixes (Bio-Rad, Berkeley, CA, USA). Mature miRNA expression analysis was performed with miRNA real-time PCR quantitation kit (Applied Biosystems, Foster City, CA, USA). Glyceraldehyde 3-phosphate (GAPDH) and U6 served as reference controls. PCR primers were as follows:

Liang H., Chen Y., Li H., Yu X., Xia C., Ming Z, & Zhong C. (2020). miR-22-3p Suppresses Endothelial Progenitor Cell Proliferation and Migration via Inhibiting Onecut 1 (OC1)/Vascular Endothelial Growth Factor A (VEGFA) Signaling Pathway and Its Clinical Significance in Venous Thrombosis. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e925482-1-e925482-9.

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Quantitative Real-Time PCR Analysis

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Total RNA was extracted using Cell Total RNA Isolation kit (Foregene, Chengdu, China) following the manufacturer’s protocol, and RNA (1 μg) was reverse‐transcribed to cDNA using the PrimeScript RT reagent kit (TaKaRa, Osaka, Japan). Quantitative real-time PCR (qPCR) was conducted using the SYBR Green qPCR Supermixes (Bio-Rad) on the CFX 192 Connect Real-Time PCR system (Bio-Rad, U.S.A.). The qPCR analysis was performed in triplicate with the primers shown in Table 2. The relative expression levels were normalized to GAPDH using the 2^−ΔΔCT method.

Zhu G., Pei L., Yang F, & Zhang C. (2022). A robust immune-related lncRNA signature for the prognosis of human colorectal cancer. Bioscience Reports, 42(7), BSR20220078.

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