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Anti ccl20 ab9829

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Anti-CCL20 (ab9829) is a recombinant antibody product targeting the chemokine CCL20. It is suitable for use in various laboratory applications such as ELISA, Western Blotting, and Immunohistochemistry.

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Lab products found in correlation

Bond polymer refine detection kit, by Leica (1 mentions) Bond max, by Leica (1 mentions)

Spelling variants of this product

Ab9829 (15 citations) CCL20 (14 citations) Anti-CCL20 (5 citations)

2 protocols using anti ccl20 ab9829

1

Immunofluorescent Staining of Cryosections

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Cryosections of tumor tissues or small intestines were incubated with the primary antibodies and fluorescent secondary antibodies. The following antibodies were used: anti-CCL20 (ab9829) from Abcam, anti-CD11 c (D1V9Y) from CST, and goat anti-rabbit IgG H&L secondary antibodies from Zsbio. The cryosections were visualized by a fluorescence microscope (Olympus BX53). Five representative fields separated over the tumor tissue were randomly selected for statistical analysis.
Li Q., Li Y., Wang Y., Xu L., Guo Y., Wang Y., Wang L, & Guo C. (2021). Oral administration of Bifidobacterium breve promotes antitumor efficacy via dendritic cells-derived interleukin 12. Oncoimmunology, 10(1), 1868122.
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2

Immunohistochemical Analysis of CCL20

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Pancreata were fixed via immersion in 10% neutral buffered formalin for 48 hours prior to paraffin processing, embedding, and sectioning at three microns. All immunohistochemistry steps were performed on a Leica Bond Max auto-immunostainer running a modified IHC protocol. Briefly, slides were dewaxed and subjected to HIER using Leica’s epitope retreival solution 2 (ER2, pH 9.0) for 20 minutes prior to primary antibody incubation (abcam anti-CCL20 [ab9829], 1:100 dilution) for 1 hr. Detection was accomplished using Leica’s Bond Polymer Refine Detection Kit and a modified “Protocol F” by incubating with anti-rabbit polymer for 8 minutes followed by a peroxide block for 5 minutes, 10 minutes of DAB reagent, and then counterstain with hematoxylin for 5 minutes.
Burke S.J., Karlstad M.D., Regal K.M., Sparer T.E., Lu D., Elks C.M., Grant R.W., Stephens J.M., Burk D.H, & J. Jason C. (2015). CCL20 is elevated during obesity and differentially regulated by NF-κB subunits in pancreatic β-cells. Biochimica et biophysica acta, 1849(6), 637-652.
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