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Mikrowin 2000

Manufactured by Berthold Technologies
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Sourced in Germany, France

Mikrowin 2000 is a software package developed by Berthold Technologies for data acquisition and analysis in laboratory settings. The software provides a platform for collecting, managing, and interpreting data from various laboratory instruments and equipment.

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Lab products found in correlation

Prism 4, by GraphPad (2 mentions) Aperio at2, by Leica (2 mentions) Prism 9, by GraphPad (2 mentions) Optiplate, by PerkinElmer (1 mentions) Centro lb 960, by Berthold Technologies (1 mentions) Gsh gssg glo assay kit, by Promega (1 mentions) Atp lite one step kit, by PerkinElmer (1 mentions) Chameleon 5 plate reader, by Hidex (1 mentions) Luciferin, by GoldBio (1 mentions) Mithras lb940 multimode reader, by Berthold Technologies (1 mentions) Asys uvm 340, by Harvard Bioscience (1 mentions) Atp assay kit, by Beyotime (1 mentions) Human neurotrophin 3 elisa kit, by Abcam (1 mentions)

Close spelling variants of this product

MikroWin 2000, Version 4.41 WinGlow Software, MikroWin 2000, Version 4.41

13 protocols using mikrowin 2000

1

Neurotensin-Induced Calcium Signaling Assay

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The assay was similarly performed as described 32 . HEK293 cells were used that expressed stably transfected NTR1 and a mitochondrial membrane localized apo-aequorin calcium reporter. Suspended cells were equilibrated with 5 μM Coelenterazine H for 2 hours by shaking at 160 rpm in a dark 37°C incubator. Ten minutes prior to measurements, cells were pre-treated with vehicle or with 10 μM of ML301 or ML314. Measurements were made by injecting approximately 50,000 cells into a well of a white OptiPlate (PerkinElmer; Waltham, MA) containing a fixed concentration of the neurotensin peptide fragment NT(8-13). Luminescence was recorded for 15 seconds using a Mithras LB940 luminescence reader running MikroWin2000 software (Berthold Technologies, Oak Ridge, TN) (N = 3).
Barak L.S., Bai Y., Peterson S.M., Evron T., Urs N.M., Peddibhotla S., Hedrick M.P., Hershberger P., Maloney P., Chung T.D., Rodriguiz R.M., Wetsel W.C., Thomas J.B., Hanson G.R., Pinkerton A.B, & Caron M.G. (2016). ML314: A Biased Neurotensin Receptor Ligand for Methamphetamine Abuse. ACS chemical biology, 11(7), 1880-1890.
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2

Quantifying IL-1β in Cell Cultures

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Interleukin-1β ELISA (Novex, Life Technologies) were used to measure levels of the proinflammatory cytokine, IL-1β, in cell culture supernatant samples, according to manufacturers’ protocol. Absorbance was read at 450 nm (with 570 nm γ-correction) using Mirthas LB940 (Berthold Technologies, Bad Wildbad, Germany) and MikroWin 2000 analysis software.
Harrison S.R., Scambler T., Oubussad L., Wong C., Wittmann M., McDermott M.F, & Savic S. (2018). Inositol-Requiring Enzyme 1-Mediated Downregulation of MicroRNA (miR)-146a and miR-155 in Primary Dermal Fibroblasts across Three TNFRSF1A Mutations Results in Hyperresponsiveness to Lipopolysaccharide. Frontiers in Immunology, 9, 173.
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3

Quantitative Luminescence Antagonist Assay

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Luminescence data (peak integration) were calculated using MikroWin 2000 software (Berthold), which was linked to the Microsoft Excel program. All statistical and curve-fitting analyses were performed using Prism 4.0 (GraphPad) software. Data are expressed in percentage (% RLU) of the maximal luminescence that was detected with 10−4 M SP (without antagonist). The competitive nature of antagonism was evaluated using the Schild plot method.40 All antagonists analyzed in this study provided linear regression plots and were considered competitive. The pA2 values were calculated using Schild's equation.49
Guillemyn K., Kleczkowska P., Lesniak A., Dyniewicz J., Van der Poorten O., Van den Eynde I., Keresztes A., Varga E., Lai J., Porreca F., Chung N.N., Lemieux C., Mika J., Rojewska E., Makuch W., Van Duppen J., Przewlocka B., Broeck J.V., Lipkowski A.W., Schiller P.W., Tourwé D, & Ballet S. (2014). Synthesis and biological evaluation of compact, conformationally constrained bifunctional opioid agonist – neurokinin-1 antagonist peptidomimetics. European journal of medicinal chemistry, 92, 64-77.
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4

Glutathione Redox State Measurement

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Reduced (GSH) and oxidized (GSSG) glutathione measurements were performed using the GSH/GSSG-Glo™ Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions. The luminescence signal was acquired using with a microplate luminometer Centro LB 960 and data were recorded using the MikroWin 2000 software package (Berthold Technologies, Bad Wildbad, Germany).
Song S., Lee J.Y., Ermolenko L., Mazumder A., Ji S., Ryu H., Kim H., Kim D.W., Lee J.W., Dicato M., Christov C., Schnekenburger M., Cerella C., Gérard D., Orlikova-Boyer B., Al-Mourabit A, & Diederich M. (2020). Tetrahydrobenzimidazole TMQ0153 triggers apoptosis, autophagy and necroptosis crosstalk in chronic myeloid leukemia. Cell Death & Disease, 11(2), 109.
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5

Luminescence-Based Antagonist Evaluation

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Luminescence data (peak integration) were calculated as described previously2 (link) using MikroWin 2000 software (Berthold). Statistical and curve-fitting analyses were performed using Prism 4.0 (GraphPad) software. Data were expressed in percentage (% RLU) of the maximal luminescence that was detected with 10−4 M SP (without antagonist). The competitive nature of antagonism was evaluated using the Schild plot method.22 (link) All antagonists analyzed in this study provided linear regression plots and were considered competitive. The pA2 values were calculated using the Schild’s equation.22 (link)
Starnowska J., Costante R., Guillemyn K., Popiolek-Barczyk K., Chung N.N., Lemieux C., Keresztes A., Van Duppen J., Mollica A., Streicher J., Broeck J.V., Schiller P.W., Tourwé D., Mika J., Ballet S, & Przewlocka B. (2017). Analgesic Properties of Opioid/NK1 Multitarget Ligands with Distinct in Vitro Profiles in Naive and Chronic Constriction Injury Mice. ACS chemical neuroscience, 8(10), 2315-2324.
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6

Multimodal Data Analysis Protocol

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MikroWin 2000 software (Berthold Technologies) was used to collect luciferase assay data. The Leica Aperio AT2 digital pathology scanning system (Leica Biosystems, Inc.) was used to collect the photomicrographs for the lung histopathological analysis. GraphPad Prism 9.4 software was used for figure preparation and statistical analyses.
Lu M., Yao W., Li Y., Ma D., Zhang Z., Wang H., Tang X., Wang Y., Li C., Cheng D., Lin H., Yin Y., Zhao J, & Zhong G. (2023). Broadly Effective ACE2 Decoy Proteins Protect Mice from Lethal SARS-CoV-2 Infection. Microbiology Spectrum, 11(4), e01100-23.
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7

Extracellular ATP Quantification Protocol

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Extracellular ATP in cell-free medium supernatant was quantified using ATP Lite one step kit (Perkin Elmer, Courtaboeuf, France) according to the manufacturer's instructions, and the luminescence produced was measured (Mithras, Mikrowin 2000 software, Berthold Technologies, Thoiry, France).
Baron L., Gombault A., Fanny M., Villeret B., Savigny F., Guillou N., Panek C., Le Bert M., Lagente V., Rassendren F., Riteau N, & Couillin I. (2015). The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine. Cell Death & Disease, 6(2), e1629-.
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8

Quantification of Type I and III IFNs

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IFN-α and IFN-β in the sera were determined by Mouse IFN-alpha and IFN-beta ELISA Kits (PBL Interferon Source, NJ, USA). IL-28 was determined by ELISA Kits (Sino-American Biotech, China). The intensities were quantitated in a Plate CHAMELEON V reader (Hidex, Finland, λ = 450 nm) with Mikrowin 2000 software (Berthold Technologies, Germany).
Chen J., Zhao Y., Zhang C., Chen H., Feng J., Chi X., Pan Y., Du J., Guo M., Cao H., Chen H., Wang Z., Pei R., Wang Q., Pan L., Niu J., Chen X, & Tang H. (2014). Persistent hepatitis C virus infections and hepatopathological manifestations in immune-competent humanized mice. Cell Research, 24(9), 1050-1066.
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9

Quantifying cAMP in HEK-293T cells

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HEK-293T cells were transfected with the GloSensor™ cAMP construct (Promega, Madison, WI) and mouse D2R-long form (mD2LR) DNA 2008 (link). On the day of the experiment, the cells were washed in Hank's Balanced Salt Solution (HBSS; Gibco, Grand Island, NY); then 25 μL of 25 mmol/L luciferin (Gold Biotechnology, St. Louis, MO) in HBSS was added to each well and incubated in the dark at room temperature for 2 h. Following incubation, the luciferin solution was aspirated and 80 μL HBSS was added, and dose–response curves were generated for quinpirole, aripiprazole, and cariprazine. Five minutes after the addition of each drug, isoproterenol (10−7 mol/L) was added to induce cAMP production for an additional five minutes. Luminescence generated from the GloSensor™ construct was measured with a Mithras LB940 Multimode Reader, using MikroWin 2000 software (Berthold Technologies, Oak Ridge, TN). All curves generated were normalized to the maximal response of isoproterenol, to control for expression differences between experiments.
Gao Y., Peterson S., Masri B., Hougland M.T., Adham N., Gyertyán I., Kiss B., Caron M.G, & El-Mallakh R.S. (2014). Cariprazine exerts antimanic properties and interferes with dopamine D2 receptor β-arrestin interactions. Pharmacology Research & Perspectives, 3(1), e00073.
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10

Multimodal Data Analysis Protocol

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MikroWin 2000 software (Berthold Technologies) was used to collect luciferase assay data. The Leica Aperio AT2 digital pathology scanning system (Leica Biosystems, Inc.) was used to collect the photomicrographs for the lung histopathological analysis. GraphPad Prism 9.4 software was used for figure preparation and statistical analyses.
Lu M., Yao W., Li Y., Ma D., Zhang Z., Wang H., Tang X., Wang Y., Li C., Cheng D., Lin H., Yin Y., Zhao J, & Zhong G. (2023). Broadly Effective ACE2 Decoy Proteins Protect Mice from Lethal SARS-CoV-2 Infection. Microbiology Spectrum, 11(4), e01100-23.
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