Proteins were isolated using RIPA lysis buffer (Beyotime, Haimen, China) and protease inhibitors (Beyotime). The concentration of extracted protein samples was analyzed with BCA kit (Beyotime), subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and then transferred to polyvinyl difluoride membranes (Beyotime). Membranes were blocked by 5% nonfat milk at room temperature for 4 hours and then incubated with primary antibodies (anti-SYF2: ab236417, anti-GAPDH: ab8245, both purchased from Abcam, Cambridge, Massachusetts) at 4°C for overnight, followed by incubation with goat anti-mouse secondary antibody (ab6789; Abcam) incubation at room temperature for 2 hour. Band signals were visualized using BeyoECL kit (Beyotime). GAPDH was used as internal control to normalize the expression of SYF2. Each sample was analyzed 3 times.
Ab236417
Manufactured by Abcam
Sourced in United Kingdom
Ab236417 is a lab equipment product manufactured by Abcam. It is designed for use in research applications. The core function of this product is to facilitate specific laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab236417
1
Western Blot Analysis of SYF2 Protein
2
Immunostaining of Cryopreserved Pellet Cultures
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Pellet cultures were cryopreserved in OTC and 5 µm sections were cut for immunostaining. Sections were fixed in 4% buffered paraformaldehyde and blocked at room temperature for 1 h with 1% goat or donkey serum. After washing with PBS, sections were incubated with rabbit-anti SOD2 (ab68155), rabbit-anti vimentin (ab92547), rabbit-anti MGST1 (ab131059) and mouse-anti SYF2 (ab236417) antibodies purchased from ©Abcam plc. (Biomedical Campus, Discovery Drive, Trumpington, Cambridge, CB2 0AX, UK). Sections were then washed and incubated with the appropriate secondary antibodies. Using confocal microscopy, IHC staining was detected, and the stained sections were analyzed for fluorescence intensity blindly.
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