The growth rate of L. rhamnosus yoba in the jam was evaluated over a period of 24 hours. Sampling was done every 2 h over the 24 h period. One milliliter (1 mL) of a sample was aseptically taken from the jam and suspended in sterile 9 ml of peptone physiological salt solution (pH 7.0, 8.5 g/L NaCl, and 1 g/L neutralized bacteriological peptone from Oxoid). Diluents of 100 μL were plated in triplicate onto de Man, Rogosa, and Sharpe (MRS) agar (1.2% agar, bacteriological peptone from Oxoid, added to de Man, Rogosa, and Sharpe broth, Merck). MRS agar plates were incubated at 37°C under anaerobic conditions in GasPak anaerobic jars (Becton Dickinson Microbiology Systems, Baltimore, Maryland, USA). All colonies on the MRS agar were counted, and the results were expressed as colony forming units per milliliter (CFU/mL) of L. rhamnosus yoba, taking into account the dilution factors.
Peptone physiological salt solution
Manufactured by Thermo Fisher Scientific
Peptone physiological salt solution is a laboratory reagent used to prepare culture media for the growth and maintenance of microorganisms. It provides a source of peptone, which serves as a source of nitrogen, carbon, and other nutrients, as well as a physiological salt solution to maintain the osmotic balance required for microbial growth.
Automatically generated - may contain errors
3 protocols using peptone physiological salt solution
1
Growth Kinetics of L. rhamnosus in Jam
2
Enumeration of Lactobacillus rhamnosus yoba
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enumeration of viable L. rhamnosus yoba was carried out by collecting 1 mL of probiotic jam under aseptic conditions. Thereafter, serial decimal dilutions were carried out in a peptone physiological salt solution (pH 7.0, 8.5 g / L NaCl, and 1 g / L neutralized bacteriological peptone from Oxoid). Diluents of 100 μL were plated in triplicate onto de Man, Rogosa and MRS agar (1.2 % agar, bacteriological peptone from Oxoid, added to de Man, Rogosa and Sharpe broth, Merck). MRS agar plates were incubated at 37 °C under anaerobic conditions in Gas Pack anaerobic jars (Becton Dickinson Microbiology Systems, Baltimore, Maryland, USA). All colonies on the MRS agar were counted and results were expressed as colony forming units per millilitre (CFU/mL) of L. rhamnosus yoba, taking into account the dilution factors.
3
Enumeration of Lb. rhamnosus yoba in Maheu
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The growth rate of Lb. rhamnosus yoba in the traditional maheu was determined at t = 0, 12, 24, and 36 h. One millilitre of the sample was aseptically taken from the maheu at a 12 hourly interval. Thereafter, serial decimal dilutions were carried out in peptone physiological salt solution (pH 7, 8.5 g / L NaCl, and 1 g / L neutralized bacteriological peptone from Oxoid). Diluents of 100 µL were plated onto de Man, Rogosa, and MRS agar (1.2 % agar, bacteriological peptone from Oxoid, added to de Man, Rogosa, and Sharpe broth, Merck) in triplicate. MRS agar plates were incubated at 37 °C under anaerobic conditions in Gas Pack anaerobic jars (Becton Dickinson Microbiology Systems, Baltimore, Maryland, USA). Colonies on MRS agar were counted and expressed as colony-forming units per milliliter (CFU / mL) of Lb. rhamnosus yoba.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!