The pH cycling method was used to test the anti-demineralization and remineralization effects.12 (link) The bracket attachment process was performed in the same way as in the SBS measurement. After the brackets were bonded, samples were stored in distilled water for 24 h, followed by a cycle of submerging the samples in demineralization solution (Biosesang, Seongnam, South Korea) for 6 h and in remineralization solution (Biosesang) for 18 h. The pH cycle was repeated for 14 days. Between the solution changes, the samples were transferred to distilled water for 1 min, washed, and dried. The solutions were replaced every seven days. After pH cycling, the samples were scanned using micro-computed tomography (micro-CT; InspXio SMX-90CT, Shimadzu, Kyoto, Japan) at 90 kV and 109 μA. The micro-CT data were analyzed using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Various sizes of CT scans were corrected using a scale bar. A brightness of 87% was defined as a sound enamel for analysis, and data exceeding the criteria were considered to have shown enamel loss. The remineralization length of each sample, defined as the distance between the end point of the orthodontic bonding resins and the sound enamel, was measured.
Inspxio smx 90ct
Manufactured by Shimadzu
Sourced in Japan
The InspXio SMX-90CT is a compact X-ray microfocus computed tomography (micro-CT) system designed for non-destructive inspection and analysis of a wide range of samples. It features a high-resolution X-ray source and a sensitive detector to capture detailed 3D images of the internal structure of the sample.
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Lab products found in correlation
3 protocols using inspxio smx 90ct
1
Anti-demineralization and Remineralization Effects
2
Synthesizing Orthodontic Bonding Resin Samples
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To create experimental orthodontic bonding resin samples, the synthesized MBN was mixed with the SAR (Ortho Connect Flow; GC Corp, Tokyo, Japan, Table 1 ) in experimental ratios of 0 wt%, 1 wt%, 3 wt%, and 5 wt%, respectively. To prevent polymerization by light during mixing, SAR and MBN were added to a 2 mL black e-tube and mixed twice, for 10 s in each round of mixing, using a mixer (mixing speed; 2850 rotations per minute, 3M ESPE, Seefeld, Germany). These experimental orthodontic bonding resin used to bond metal brackets and make discs. After that light (1000 mW/cm2, VALO; Ultradent, South Jordan, UT, USA) was cured for 20 s on each tooth and discs. The characterization of the fabricated discs fabricated discs (10 mm) was analyzed by XRD and FTIR. The MBN placement in the resin structure was evaluated using the field emission scanning electron microscope (FE-SEM, S-4300, HITACHI, Tokyo, Japan) and micro-computed tomography (InspXio SMX-90CT, Shimadzu, Kyoto, Japan) at 90 kV, 109 μA condition.
To compare the mechanical and biological properties of the experimental groups, two different sizes of resin discs were fabricated (5 mm and 10 mm diameters and 1 mm height,Figure 1 ).
To compare the mechanical and biological properties of the experimental groups, two different sizes of resin discs were fabricated (5 mm and 10 mm diameters and 1 mm height,
3
Anti-Demineralization and Remineralization of Orthodontic Bonding Resins
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The pH cycling method was used to test the anti-demineralization and remineralization effects of the experimental orthodontic bonding resins [21 (link)]. After the bracket was bonded, the samples were stored in distilled water for 24 h, followed by a cycle of submerging samples in demineralization solution (Biosesang, Seongnam, Korea) for 6 h, and 18 h in remineralization solution (Biosesang, Seongnam, Korea), which was repeated for 14 days. Between the solution changes, the samples were transferred to distilled water for 1 min, washed, and dried. The solutions were replaced every seven days. After pH cycling, samples were scanned using micro-computed tomography (micro CT; InspXio SMX-90CT, Shimadzu, Kyoto, Japan) at 90 kV, 109 μA condition. Micro CT data were analyzed using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Various sizes of scanned CTs were corrected using a scale bar. To account for sound enamel in the data, a brightness of 87% was defined as sound enamel, and data exceeding the criteria were considered to have shown enamel loss. The remineralization length of each sample was measured, which was defined as the end point of orthodontic bonding resins to the accounted sound enamel.
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