Arabidopsis guard cell protoplasts of leave 5 were isolated as described previously (Tallman, 2006 (link); Zhang et al., 2008 (link)). The whole-cell voltage-clamp or single-channel currents of Arabidopsis guard cells were recorded with an EPC-9 amplifier (Heka Instruments), as described previously (Bai et al., 2009 (link)). Pipettes were pulled with a vertical puller (Narishige, Japan) modified for two-stage pulls. Data were analyzed using PULSEFIT 8.7 software. Standard solutions for Ca2+ measurements were used, including 10 mM BaCl2, 0.1 mM DTT, 10 mM MES-Tris (pH 5.6) in a bath, and 100 mM BaCl2, 0.1 mM DTT, 4 mM EGTA, and 10 mM HEPES-Tris (pH 7.1) in a pipette. ABA was freshly added to bath solutions at the indicated concentrations. For ABA-activated Ca2+ current measurements, 50 μM ABA was added to the standard pipette solution. Osmolalities of pipette and bath solutions were adjusted to 510 and 490 mM kg−1, respectively, using D-sorbitol (Sangon, China).
D sorbitol
Manufactured by Sangon
Sourced in China
D-sorbitol is a six-carbon sugar alcohol that serves as a common sweetener and humectant in various industries. It is commonly used in the production of pharmaceuticals, cosmetics, and food products.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using d sorbitol
1
Arabidopsis Guard Cell Electrophysiology
2
Yeast Pre-Culture and Sorbitol Adaptation
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In the first pre-culture, cells were incubated in a fresh YPD Agar media (YPD Agar, A507023, Sangon Biotech, Shanghai, China) for 16 h at 30 • C. Cells were then inoculated in a second pre-culture in 6 mL YPD-media (YPD broth, A507022, Sangon Biotech, Shanghai, China) and cultured to the logarithmic phase of growth at 30 • C and 220 rpm of agitation. Yeast cell culture was centrifuged at 3,000 × g for 5 min, followed by washing three times with sorbitol buffer and resuspending in sorbitol culture medium (1 M sorbitol solution in YPD broth). The sorbitol buffer was composed of 1.2 M sorbitol solution (D-Sorbitol, A100691, Sangon Biotech, Shanghai, China) in 0.1 M KH 2 PO 4 (Potassium dihydrogen phosphate, A100781, Sangon Biotech, Shanghai, China).
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