AFM images were recorded using a Fast Scan head (Bruker) at 0.5 Hz line rate using ScanAsyst Fluid cantilevers (Bruker) with a nominal spring constant of 0.7 N m-1 in PeakForce quantitative nanomechanical mode (QNM) at an oscillation rate of 1 kHz and a force setpoint < 2 nN. Channels recorded include height sensor, peak force error, and DMT modulus. The abrupt height change during cell cleavage was measured by imaging in peak force tapping node at the same spot (0 nm scan size) on the PCF and data were recorded at a rate of 1 kHz. AFM imaging of stiffness at the PCF was done by repeatedly scanning the same line across the PCF at 1 line per second, at a slightly elevated force setpoint (5 nN). The highest stiffness value from each scan line was plotted over time. Before force curves on the PCF were recorded, a small area on the PCF was scanned to determine its center. To force cell cleavage in RipA-depleted cells, the line along the septum was scanned repeatedly and the force setpoint was increased gradually in order to increase the applied pressure.
Fast scan head
Manufactured by Bruker
The Fast Scan Head is a laboratory equipment component used for high-speed scanning applications. It is designed to provide precise and rapid positioning of samples or detectors within a given measurement setup.
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Lab products found in correlation
2 protocols using fast scan head
1
Nanomechanical Characterization of Bacterial Cell Division
2
Nanomechanical Characterization of Bacterial Cell Division
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AFM images were recorded using a Fast Scan head (Bruker) at 0.5 Hz line rate using ScanAsyst Fluid cantilevers (Bruker) with a nominal spring constant of 0.7 N m-1 in PeakForce quantitative nanomechanical mode (QNM) at an oscillation rate of 1 kHz and a force setpoint < 2 nN. Channels recorded include height sensor, peak force error, and DMT modulus. The abrupt height change during cell cleavage was measured by imaging in peak force tapping node at the same spot (0 nm scan size) on the PCF and data were recorded at a rate of 1 kHz. AFM imaging of stiffness at the PCF was done by repeatedly scanning the same line across the PCF at 1 line per second, at a slightly elevated force setpoint (5 nN). The highest stiffness value from each scan line was plotted over time. Before force curves on the PCF were recorded, a small area on the PCF was scanned to determine its center. To force cell cleavage in RipA-depleted cells, the line along the septum was scanned repeatedly and the force setpoint was increased gradually in order to increase the applied pressure.
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