αmem medium

All protocols and animal procedures detailed below were approved by the Ethics Committees of Lu He Hospital, Capital Medical University, and were performed following the principles of Management Rules of the Ministry of Health of China. The isolation and passage of hUC-MSCs was done following the methods of Bharti et al^{15 (link)}
. The umbilical cord was washed with phosphate buffer saline (PBS) at 4°C, cut into 1 to 2 cm small pieces, and the umbilical cord arteries and veins were removed (Fig. 1A). After removing 1 umbilical vein and 2 umbilical arteries, carefully bluntly separated the Wharton’s jelly, and then mechanically cut them into 1 mm³ fragments with ophthalmic scissors. The pellets obtained were seeded in 10-cm culture dish supplemented with α-MEM medium (Biological Industries Israel Beit Haemek Ltd, Kibbutz Beit-Haemek, Israel) supplemented with 10% fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 μg/mL). The cells were cultured to 80% to 90% confluence in incubator at 37°C and 5% CO₂. In the same culture media, the same procedure was continued until they attained the fifth passage.

NK92 cells were a kind
gift from Prof. Angel
Porgador from the Faculty of Health Sciences at Ben-Gurion University.
The melanoma cell line 888 was cultured in DMEM and the K562 cell
line was cultured in RPMI (Biological Industries, Beth Haemek, Israel),
supplemented with 10% heat-inactivated fetal bovine serum (FBS; Biological
Industries, Beth Haemek, Israel), and maintained in a 5% CO₂ incubator at 37 °C. NK92 cells were cultured in α-MEM
medium (Biological Industries, Beth Haemek, Israel) supplemented with
15% FBS, 15% horse serum, 10% myo-inositol (Biological Industries,
Beth Haemek, Israel), 10% MEM sodium pyruvate (Biological Industries,
Beth Haemek, Israel), and 200 IU/mL IL-2 and maintained at 37 °C
in a 5% CO₂ incubator.