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E300 microscope

Manufactured by Nikon
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The E300 microscope is a high-performance optical instrument designed for laboratory use. It features a stable and precise optical system that delivers clear, detailed images. The E300 is capable of various magnification levels to accommodate a wide range of sample sizes and observation needs.

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Lab products found in correlation

Magnifire digital camera, by Nikon (2 mentions) Bromodeoxyuridine (brdu), by Merck Group (2 mentions) Blocking solution, by Thermo Fisher Scientific (2 mentions) Mouse monoclonal anti brdu antibody, by Roche (2 mentions) Bromodeoxyuridine (brdu), by Nikon (2 mentions)

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E300 inverted fluorescence microscope (2 citations)

3 protocols using e300 microscope

1

Quantifying Neurogenesis via BrdU Labeling

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BrdU (50 mg/kg; Sigma) in saline was given i.p. two times daily at 8-hr intervals on consecutive days (days 1–3 after PQ or saline administration). Mice were sacrificed on day 14 after the last injection. Brains were removed following 4% paraformaldehyde in PBS. Adjacent 50-μm sections were cut and stored at −80°C. BrdU detection was performed as described previously (Peng et al., 2008 (link)). Briefly, sections were incubated with 2 μg/mL mouse monoclonal anti-BrdU antibody (1:200, Roche) at 4°C overnight, washed with PBS, incubated with Alexa 488 anti-mouse secondary antibody (1:250) in blocking solution (Life Technologies) for 1 hr, and then washed and mounted on gelatin-coated slides with Fluoromount. SVZ BrdU+ cells were counted blinded in five 50-μm coronal sections per mouse spaced 200 μm apart under high power (200×) using a Nikon E300 microscope with a Magnifire digital camera. Results are expressed as the average number of BrdU+ cells per section.
Chinta S.J., Woods G., Demaria M., Rane A., Zou Y., McQuade A., Rajagopalan S., Limbad C., Madden D.T., Campisi J, & Andersen J.K. (2018). Cellular Senescence Is Induced by the Environmental Neurotoxin Paraquat and Contributes to Neuropathology Linked to Parkinson’s Disease. Cell reports, 22(4), 930-940.
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2

Quantifying Neurogenesis via BrdU Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
BrdU (50 mg/kg; Sigma) in saline was given i.p. two times daily at 8-hr intervals on consecutive days (days 1–3 after PQ or saline administration). Mice were sacrificed on day 14 after the last injection. Brains were removed following 4% paraformaldehyde in PBS. Adjacent 50-μm sections were cut and stored at −80°C. BrdU detection was performed as described previously (Peng et al., 2008 (link)). Briefly, sections were incubated with 2 μg/mL mouse monoclonal anti-BrdU antibody (1:200, Roche) at 4°C overnight, washed with PBS, incubated with Alexa 488 anti-mouse secondary antibody (1:250) in blocking solution (Life Technologies) for 1 hr, and then washed and mounted on gelatin-coated slides with Fluoromount. SVZ BrdU+ cells were counted blinded in five 50-μm coronal sections per mouse spaced 200 μm apart under high power (200×) using a Nikon E300 microscope with a Magnifire digital camera. Results are expressed as the average number of BrdU+ cells per section.
Chinta S.J., Woods G., Demaria M., Rane A., Zou Y., McQuade A., Rajagopalan S., Limbad C., Madden D.T., Campisi J, & Andersen J.K. (2018). Cellular Senescence Is Induced by the Environmental Neurotoxin Paraquat and Contributes to Neuropathology Linked to Parkinson’s Disease. Cell reports, 22(4), 930-940.
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3

Neurite Growth Quantification Assay

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Frontiers cells were seeded in 24-well plates to measure neurite growth for 24 h and imaged using a Nikon E300 microscope. The wells were photographed in five randomly selected fields at ×100 magnification for analysis. Images were analyzed using the ImageJ software.
Kim Y., Jung Y.H., Park S.B., Kim H., Kwon J.Y., Kim H.K., Lee H.J., Jeon S, & Kim E. (2022). TMI-1, TNF-α-Converting Enzyme Inhibitor, Protects Against Paclitaxel-Induced Neurotoxicity in the DRG Neuronal Cells In Vitro. Frontiers in Pharmacology, 13, 842779.
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