RA-FLS were kept in serum-free medium for 24 h before small interfering RNA (siRNA) treatment with DharmaFECT siRNA transfection reagent and siRNA against GAPDH, KCNMB1, or KCNMB3 (GE Dharmacon, Lafayette, CO) as described previously [12 (link)]. Cells were used 40–80 h later.
Dharmafect sirna transfection reagent
Manufactured by Horizon Discovery
Sourced in United States
DharmaFECT is a cationic lipid-based transfection reagent that facilitates the delivery of small interfering RNA (siRNA) into mammalian cells for gene silencing applications. It is designed to efficiently complex and deliver siRNA into the target cells.
Automatically generated - may contain errors
Lab products found in correlation
Rna oligonucleotides, by Santa Cruz Biotechnology (5 mentions)
Rna oligonucleotide, by Horizon Discovery (3 mentions)
Rna oligonucleotides, by GenePharma (1 mentions)
Bez235, by Chemietek (1 mentions)
Sigenome smartpool sirna reagent, by Horizon Discovery (1 mentions)
Rpmi 1640, by Sartorius (1 mentions)
Siglo red, by Horizon Discovery (1 mentions)
15 protocols using dharmafect sirna transfection reagent
1
siRNA Knockdown in RA-FLS
2
Mouse Id2 Knockdown Protocol
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The RNA oligonucleotides that specifically target mouse Id2 were purchased from Thermo Dharmacon. The RNA oligonucleotides were transfected with the DharmaFECT siRNA transfection reagent (Dharmacon).
3
Silencing STAT3 and β‐Arrestin1 in Cells
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The RNA oligonucleotides that specifically targeted rat STAT3, β‐arrestin1 were purchased from GenePharma (Shanghai, China). The RNA oligonucleotides were transfected with the Dharma‐FECT siRNA transfection reagent (Dharmacon). After siRNA transfection for 24 or 48 h, cells were harvested and analyzed by western blotting and qRT‐PCR.
4
Transient SIRPB1 Knockdown in PC3 Cells
5
Targeted siRNA Knockdown of p68 and p53
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The RNA oligonucleotides that specifically target mouse p68 and mouse p53 were purchased from Santa Cruz Biotechnology, Inc. The RNA oligonucleotides were transfected with Dharma-FECT siRNA transfection reagent (Dharmacon). 24 h or 48 h after transfection, cells were harvested and analyzed by Western blotting.
6
Fas Gene Silencing by siRNA Transfection
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Disc cells were grown to reach 80%–85% confluency on the day of transfection. GeneBank accession no. for Fas was NM139194. The following siRNA constructs were used in this study: Fas siRNA sense, 5′-GAAACGAACUGCACCCGGAU-3′; negative siRNA sense, 5′-UAGCGACUAAACACAUCAA-3′ (Dharmacon, Thermo Fisher Scientific). Briefly, cells were transfected with siRNA using the DharmaFECT siRNA transfection reagent (Dharmacon) according to the manufacturer's instructions. Transfections were performed with serum-free α-modified Eagle's medium containing 200 nmol siRNA and 6 µL DharmaFECT. After 18 hours, the transfection medium was replaced with a complete medium, and the cells were collected at 48 hours for analysis of Fas silencing by reverse transcription polymerase chain reaction (RT-PCR).
7
Targeted Knockdown of Prdx5 in Cells
8
Targeted SMYD2 Knockdown Assay
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The RNA oligonucleotides that specifically targeted human SMYD2 was purchased from Santa Cruz Biotechnology, Inc. The RNA oligonucleotides were transfected with DharmaFECT siRNA transfection reagent (Dharmacon). 24 and 48 h after transfection, cells were harvested and analyzed by Western blotting.
9
siRNA-Mediated Knockdown of NRP1
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A pool of 5 different NRP1 siRNAs was purchased from Dharmacon (Dharmacon, Lafayette, CO, USA) and transfection was performed according to the manufacturer’s instruction at a final concentration of 25 nM for the NRP1 siRNA. A pool of 4 different non-targeting (nt) siRNAs was used as negative control. DharmaFECT siRNA Transfection Reagent (Dharmacon, Lafayette, CO, USA) was applied for the transfection procedure. At 24–48 h after transfection, the cells were harvested for RNA or protein extraction or trypsinized and re-seeded for functional assays.
10
siRNA Knockdown of Smyd2, CDK4, CDK6
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The RNA oligonucleotides that specifically targeted human or mouse Smyd2, CDK4, and CDK6 were purchased from Santa Cruz Biotechnology Inc. The RNA oligonucleotides were transfected with DharmaFECT siRNA transfection reagent (Dharmacon). Twenty-four hours and 48 hours after transfection, cells were harvested and analyzed by Western blotting.
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