Samples were processed as previously described3 (link),24 . In short, samples were osmicated, stained en bloc with uranyl acetate and embedded in EMbed 812, an Epon-812 substitute (EMS). 1 µm sections were cut and stained with toluidine blue and visualized on a light microscope (Leica DM5500B). Additional thin sections were cut, carbon-coated and imaged either on JEOL JEM-1200-EX electron microscope or a T12 electron microscope (FEI).
Jem 1200 ex electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The JEM-1200-EX is a transmission electron microscope (TEM) manufactured by Thermo Fisher Scientific. It is designed to provide high-resolution imaging of samples at the nanoscale level. The core function of this instrument is to produce detailed images of the internal structure and morphology of materials, enabling users to study and analyze a wide range of specimens at the atomic and molecular scale.
Automatically generated - may contain errors
Lab products found in correlation
T12 electron microscope, by Thermo Fisher Scientific (2 mentions)
Dm5500b, by Leica (2 mentions)
Tecnai g2 f20 s twin electron microscope, by Thermo Fisher Scientific (2 mentions)
Uv 2600, by Shimadzu (2 mentions)
Ftir spectrophotometer, by Shimadzu (1 mentions)
Ls 55 luminescence spectrometer, by PerkinElmer (1 mentions)
Iraffinity 1s spectrophotometer, by Shimadzu (1 mentions)
Agilent 7500ce, by Agilent Technologies (1 mentions)
6 protocols using jem 1200 ex electron microscope
1
Sample Preparation for Electron Microscopy
2
Ultrastructural Analysis of Middle Ear Mucosa
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Mice were sacrificed and cardiac perfused by 2% glutaraldehyde. Fixed middle ear mucous epithelium were separated and dehydrated in ascending concentrations of ethanol, embedded in Epon resin and sectioned. Ultrathin sections were used to determine the right position. Ultrathin sections were stained with lead citrate and uranyl acetate, and examined using a JEM-1200 EX electron microscope (FEI, Hillsboro, USA).
3
Multianalytical Characterization of Fluorescent Water-Soluble Carbon Dots
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The fluorescence response and UV absorption behavior were observed using an LS55 luminescence spectrometer (Perkin-Elmer, Waltham, MA, USA) and UV–visible spectrophotometer (UV-2600, Shimadzu, Kyoto, Japan). The transmission electron microscopy (TEM) images of FW-CDs were obtained by a JEM-1200EX electron microscope with an accelerating voltage of 120 kV. Besides this, high-resolution TEM (HR-TEM) images were acquired through an FEI Tecnai G2 F20 S-Twin electron microscope. The chemical composition of the FW-CDs was analyzed by an ESCALAB 250Xi X-ray photoelectron spectrometer (XPS, Thermo Electron, Waltham, MA, USA). The Fourier transform infrared (FTIR) spectra were obtained using an FT-IR spectrophotometer (Shimadzu, Japan). The X-ray diffraction (XRD) results were obtained using a Bruker D8 ADVANCE diffractometer. Keeping the excitation wavelength at 375 nm, the fluorescence decay time was monitored using a luminescence spectrometer (FLS1000, Edinburgh, Livingston, UK). An Agilent 730 ICP-OES system was utilized to determine the Cr(VI) and Fe3+ concentrations in environmental water samples, iron supplements and industrial effluent.
4
Comprehensive Characterization of N, S-Codoped Carbon Dots
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UV-visible absorption and fluorescence spectra were recorded using a UV-visible spectrophotometer (UV-2600, Shimadzu, Japan) and an LS55 luminescence spectrometer (Perkin-Elmer, USA), respectively. The elemental compositions of N, S-codoped CDs were obtained using an ESCALAB 250Xi X-ray photoelectron spectrometer (XPS, Thermo Electron, USA). Fourier transform infrared (FTIR) spectra were collected using the potassium bromide pellet methodology with an IRAffinity-1S spectrophotometer (Shimadzu, Japan). Transmission electron microscopy (TEM) and high-resolution TEM (HRTEM) images were obtained using a JEOL JEM-1200EX electron microscope operating at 120 kV with an accelerating voltage of 120 kV and an FEI Tecnai G2 F20 S-Twin electron microscope operating at 200 kV, respectively. The further determination of the concentration of Fe3+ in human serum was performed using an Agilent 7500ce inductively coupled plasma mass spectrometry (ICP-MS) system (Agilent Technologies, Japan).
5
Electron Microscopy of HEI-OC1 Cells
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HEI-OC1 cells were collected by trypsinization and were immediately fixed with 3% glutaraldehyde fixative solution (pH 7.4) for 1 h followed by 1% osmic acid (OsO4) in 0.1 M sodium cacodylate buffer (pH 7.2) for 1–2 h. The cells were then dehydrated with acetone and embedded in araldite CY212. Ultrathin sections (70 nM) were cut with a diamond knife, mounted on EM grids, stained with Reynolds’ lead citrate solution, gently washed with distilled water, dried, and imaged using a JEM-1200 EX electron microscope (FEI, Hillsboro, OR, United States).
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Sample Preparation for Electron Microscopy
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