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Rat anti mouse ige hrp

Manufactured by Southern Biotech
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Rat anti-mouse IgE-HRP is a reagent used in immunoassay applications. It consists of an anti-mouse IgE antibody conjugated to horseradish peroxidase (HRP), a commonly used enzyme label. This product can be used to detect and quantify mouse IgE in biological samples.

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Lab products found in correlation

Mouse slpi, by R&D Systems (2 mentions) Human slpi, by R&D Systems (2 mentions) Cat no mca2259, by Bio-Rad (2 mentions) Il 17a, by BioLegend (2 mentions) High binding 96 well plate, by Corning (1 mentions) Goat anti mouse igg1 hrp, by Thermo Fisher Scientific (1 mentions) Rat anti mouse igg1 hrp, by Southern Biotech (1 mentions) Goat anti mouse ige, by Southern Biotech (1 mentions) Hrp conjugated goat anti mouse igg, by Southern Biotech (1 mentions) Goat anti mouse igg1, by Southern Biotech (1 mentions) Goat anti mouse igg2a, by Southern Biotech (1 mentions) Imagequant las 4000 mini, by GE Healthcare (1 mentions) Pvdf membrane, by Merck Group (1 mentions) 2 4 dinitrophenylhydrazine dnph, by Merck Group (1 mentions) Enhanced chemi luminescence (ecl), by PerkinElmer (1 mentions)

Spelling variants of this product

Rat anti-mouse IgE (8 citations) Anti-IgE (5 citations) Anti-mouse IgE-HRP (4 citations) Anti-mouse IgE (3 citations)

6 protocols using rat anti mouse ige hrp

1

Quantitative ELISA for Peanut-specific IgE and IgG1

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Peanut-specific IgE and IgG1 were measured by ELISA. High binding 96-well plates (Corning) were coated with CPE (20 μg/mL) in coating buffer at 4 °C overnight. Coated plates were blocked with FBS (10%) in PBS for 2 h at room temperature. Plates were washed and incubated with serum samples overnight at 4 °C. After washing, detection antibody (rat anti-mouse IgE-HRP (Southern Biotech) or goat anti-mouse IgG1-HRP (Thermo Fisher Scientific)) was added and incubated for 2 h at room temperature. O-phenylenediamine (OPD) was used to develop the assay and H2SO4 (3 N) was added to stop the reaction for absorbance reading at 492 nm.
Angelina A., Pérez-Diego M., López-Abente J., Rückert B., Nombela I., Akdis M., Martín-Fontecha M., Akdis C, & Palomares O. (2021). Cannabinoids induce functional Tregs by promoting tolerogenic DCs via autophagy and metabolic reprograming. Mucosal Immunology, 15(1), 96-108.
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2

Measurement of ST-specific Antibodies and Cytokines

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Individual serum samples or cell culture supernatants were assayed for ST-specific IgE and IgG1 antibody response as described38 (link). Plates were coated with ST (5 μg/ml) in carbonate/bicarbonate buffer (pH 9.6) overnight at 4 °C. The secondary antibodies used in the ELISA tests were rat anti-mouse IgE-HRP (1:8000, SouthernBiotech) and rat anti-mouse IgG1-HRP (1:8000, SouthernBiotech).
The levels of IFN-γ, IL-4, IL-5, IL-10, IL-13, IL-6 and IL-17A in the cell culture supernatants were measured by ELISA kits according to manufacturers’ instructions (eBioscience, San Diego, USA). The mTOR activity in the cell lysates were analyzed by mTOR (pSer2448) ELISA kit (Abcam).
Fu L., Peng J., Zhao S., Zhang Y., Su X, & Wang Y. (2017). Lactic acid bacteria-specific induction of CD4+Foxp3+T cells ameliorates shrimp tropomyosin-induced allergic response in mice via suppression of mTOR signaling. Scientific Reports, 7, 1987.
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3

Antibody-based Immunological Analyses

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The mAbs used for flow cytometric analysis and other experiments were obtained from eBioscience (San Diego, CA, USA) or BD Biosciences (San Diego, CA, USA): FITC-labeled anti-mouse CD4 (RM4-5), CD45 (30-F11); PE-labeled anti-mouse IFN-γ (XMG1.2), FoxP3 (FJK-16s); PerCP-labeled anti-mouse CD4 (L3T4, RM4-5); allophycocyanin (APC)-conjugated anti-mouse IL-17A (eBio17B7); biotin-conjugated anti-mouse IL-4 (BVD6-24G2), and streptavidin-conjugated PerCP. PMA and ionomycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The Abs used to detect OVA-specific-IgE, IgG, IgG1, and IgG2a levels in sera were purchased from Southern Biotech (Birmingham, AL, USA): unconjugated (human absorbed) anti-mouse IgG, goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgE, HRP-conjugated goat anti-mouse IgG, goat anti-mouse IgG1, goat anti-mouse IgG2a, and rat anti-mouse IgE-HRP. The primers specific for cytokines, mucins, and tight junctions (TJs) were synthesized by Bioneer Corp. (Daejeon, Korea) and used for PCR amplification of target genes.
Hossain F.M., Park S.O., Kim H.J., Eo J.C., Choi J.Y., Tanveer M., Uyangaa E., Kim K, & Eo S.K. (2021). Indoleamine 2,3-Dioxygenase in Hematopoietic Stem Cell-Derived Cells Suppresses Rhinovirus-Induced Neutrophilic Airway Inflammation by Regulating Th1- and Th17-Type Responses. Immune Network, 21(4), e26.
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4

Protein Carbonyl Content Quantification

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For determination of the protein carbonyl content ~20 mg of liver tissue was lysed in 400 μl SDS-sample buffer. 50 μg homogenate was transferred to a new tube, supplemented with SDS to a total concentration of 12%, and incubated for 30 minutes at room temperature with 2 volumes of 20 mM 2,4-Dinitrophenylhydrazine (DNPH; Sigma-Aldrich) in 10% Trifluoroacetic acid (TCA) or 2 volumes 10% TCA only as negative control. The reaction was neutralized with 1.7 starting volume of 2M Tris base containing 30% glycerol and the protein samples were transferred by dotblot to PVDF membrane (Millipore). Carbonylation was visualized with anti-DNPH (Sigma-Aldrich; D8406; 1:2,000) in 5% BSA in PBS-Tween 0.05% and rat anti-mouse IgE-HRP (SouthernBiotech; 1130–05; 1:5,000), using ECL+ (PerkinElmer) on the ImageQuant LAS4000 mini (GE Healthcare Life Sciences). Signal intensities were quantified in ImageQuant TL software and corrected for total protein by Ponceau S. All samples were analyzed at least in duplo.
La Fata G., van Vliet N., Barnhoorn S., Brandt R.M., Etheve S., Chenal E., Grunenwald C., Seifert N., Weber P., Hoeijmakers J.H., Mohajeri M.H, & Vermeij W.P. (2017). Vitamin E Supplementation Reduces Cellular Loss in the Brain of a Premature Aging Mouse Model. The journal of prevention of Alzheimer's disease, 4(4), 226-235.
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5

Quantification of Serum OVA-Specific IgE

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IL-17A (Biolegend) and mouse SLPI (R&D Systems DY1735–05), and human SLPI (R&D Systems DY1274–05) ELISAs were performed according to the manufacturer’s protocol. Mouse serum OVA-specific IgE was quantified by sandwich ELISA (Zuberi et al., 2000 (link)). Briefly, plates were coated with 10μg/mL of OVA overnight at 4°C and then blocked with PBS 1x + 0.1% BSA. Serum (1:25 dilution) was placed in the wells and incubated for 2 h at room temperature. OVA-specific monoclonal IgE (Bio-Rad Cat No. MCA2259. Clone 2C6) was used as the standard curve. The bound IgE was detected by Rat anti-mouse IgE-HRP (Southern Biotech Cat No. 1130–05. Clone 23G3).
Jaeger N., McDonough R.T., Rosen A.L., Hernandez-Leyva A., Wilson N.G., Lint M.A., Russler-Germain E.V., Chai J.N., Bacharier L.B., Hsieh C.S, & Kau A.L. (2020). Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell reports, 33(5), 108331.
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6

Quantification of Serum OVA-Specific IgE

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IL-17A (Biolegend) and mouse SLPI (R&D Systems DY1735–05), and human SLPI (R&D Systems DY1274–05) ELISAs were performed according to the manufacturer’s protocol. Mouse serum OVA-specific IgE was quantified by sandwich ELISA (Zuberi et al., 2000 (link)). Briefly, plates were coated with 10μg/mL of OVA overnight at 4°C and then blocked with PBS 1x + 0.1% BSA. Serum (1:25 dilution) was placed in the wells and incubated for 2 h at room temperature. OVA-specific monoclonal IgE (Bio-Rad Cat No. MCA2259. Clone 2C6) was used as the standard curve. The bound IgE was detected by Rat anti-mouse IgE-HRP (Southern Biotech Cat No. 1130–05. Clone 23G3).
Jaeger N., McDonough R.T., Rosen A.L., Hernandez-Leyva A., Wilson N.G., Lint M.A., Russler-Germain E.V., Chai J.N., Bacharier L.B., Hsieh C.S, & Kau A.L. (2020). Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell reports, 33(5), 108331.
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