GFP-expressing AAV or lentiviral vectors were administrated i.n. into hACE2 K18 Tg (AAV) or SAMHD1 KO mice (lentivirus). At 3-dpi, the lungs were homogenized in ACK buffer and the cells were disaggregated by a 30-min treatment with 1.5 mg/mL collagenase and 0.1 mg/mL DNase followed by passage through a 100-μm mesh. The cells were blocked with anti-CD16/CD32 (RRID:AB_1574975) and stained with Alexa 700-anti-CD45 (RRID:AB_493715), PerCP-Cy5.5-anti-F4/80 (RRID:AB_893496), APC-Cy7-SiglecF (RRID:AB_2904295), PE-Cy7-anti-CD11c (RRID:AB_493569), PE-Cy7-anti-CD19 (RRID:AB_313655), APC-anti-CD3 (RRID:AB_2561456), Pacblue-anti-CD11b (RRID:AB_755985), PE-Cy5.5-anti-CD62L (RRID:AB_313097), APC-anti-CD14 (RRID:AB_940574) and PE-Ly6C/Ly6G (Gr1) (RRID:AB_313372) (BioLegend) and analyzed on a Beckman CytoFLEX flow cytometer using with FlowJo software. Cell types were classified as epithelial (CD45−), alveolar macrophages (CD45+, F4/80+, SiglecF+), interstitial macrophages (CD45+, F4/80+, SiglecF−), DCs (CD45+, F4/80−, CD11c+), T cells (CD45+, CD3+), B cells (CD45+, CD19+), monocytes (CD45+, CD11b+, CD14+) and neutrophils (CD45+, CD62L+, Ly6C/Ly6G+). LY-CoV1404 was obtained from discarded vials of Bebtelovimab.
Percp cy5.5 anti f4 80
Manufactured by BioLegend
Sourced in United States
PerCP-Cy5.5-anti-F4/80 is a conjugated antibody used for the identification and quantification of F4/80-expressing cells in flow cytometry applications. It consists of an anti-F4/80 antibody coupled to the PerCP-Cy5.5 fluorescent dye, which allows for the detection of F4/80-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd19 pe cy7, by BioLegend (1 mentions)
Anti cd11c pe cy7, by BioLegend (1 mentions)
Anti cd16 cd32, by BioLegend (1 mentions)
Anti cd3 apc, by BioLegend (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Anti cd3 fitc, by BioLegend (1 mentions)
Anti cd4 apc, by BioLegend (1 mentions)
Anti cd45 pe cy7, by BioLegend (1 mentions)
Anti cd11b apc cy7, by BioLegend (1 mentions)
Anti cd11b fitc, by BD (1 mentions)
Percp cy5.5 anti cd8, by BioLegend (1 mentions)
2 protocols using percp cy5.5 anti f4 80
1
Profiling Immune Cell Composition in Mouse Lungs
2
Multicolor Flow Cytometry Analysis
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The fluorescence-labeled monoclonal antibodies were as follows: FITC-anti-CD3, PerCp-Cy5.5-anti-CD8, APC-Cy7-anti-CD11b, PerCp-Cy5.5-anti-F4/80, APC-anti-CD4, and PE-Cy7-anti-CD45 were purchased from Biolegend (San Diego, CA, USA); FITC-anti-CD11b was from BD Biosciences (San Jose, CA, USA); and PerCp-Cy5.5-anti-F4/80 was from eBioscience (San Diego, CA, USA). MNCs were stained with the indicated antibodies as previously described37 (link). Data were collected on a FACSCalibur flow cytometer (BD Biosciences) and analyzed using FlowJo 7.6.1 (Tree Star, Ashland, OR).
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